scholarly journals Identification and Removal of Potential Contaminants in 16S rRNA Gene Sequence Data Sets from Low-Microbial-Biomass Samples: an Example from Mosquito Tissues

mSphere ◽  
2021 ◽  
Author(s):  
Sebastián Díaz ◽  
Juan S. Escobar ◽  
Frank W. Avila
Keyword(s):  
Microbial Biomass ◽  
Gene Sequence ◽  
Sequence Data ◽  
Taxonomic Composition ◽  
Rrna Gene ◽  
Data Sets ◽  
Rrna Gene Sequence ◽  
Tissue Samples ◽  
Laboratory Environment ◽  
Gene Sequence Data

The study of tissue-associated microbiota from mosquitoes (primarily from the gut) has grown significantly in the last several years. Mosquito tissue samples represent a challenge for researchers given their low microbial biomass and similar taxonomic composition commonly found in the laboratory environment and in molecular reagents.

scholarly journals Molecular phylogeny of the heterocystous cyanobacteria (subsections IV and V) based on nifD

2004 ◽  
Vol 54 (2) ◽  
pp. 493-497 ◽  
Author(s):  
Brian J. Henson ◽  
Sharon M. Hesselbrock ◽  
Linda E. Watson ◽  
Susan R. Barnum
Keyword(s):  
Phylogenetic Analysis ◽  
Gene Sequence ◽  
Sequence Data ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Cellular Division ◽  
Heterocystous Cyanobacteria ◽  
Nitrogen Fixation Gene ◽  
True Branching ◽  
Gene Sequence Data

The heterocystous cyanobacteria are currently placed in subsections IV and V, which are distinguished by cellular division in one plane (false branching) and in more than one plane (true branching), respectively. Published phylogenies of 16S rRNA gene sequence data support the monophyly of the heterocystous cyanobacteria, with members of subsection V embedded within subsection IV. It has been postulated that members of subsection V arose from within subsection IV. Therefore, phylogenetic analysis of nucleotide sequences of the nitrogen-fixation gene nifD from representatives of subsections IV and V was performed by using maximum-likelihood criteria. The heterocystous cyanobacteria are supported as being monophyletic, with the non-heterocystous cyanobacteria as their closest relative. However, neither subsection IV nor subsection V is monophyletic, with representatives of both subsections intermixed in two sister clades. Analysis of nifD does not support recognition of two distinct subsections.

Reassessment of the classification of the Order Haplosclerida (Class Demospongiae, Phylum Porifera) using 18S rRNA gene sequence data

2007 ◽  
Vol 43 (1) ◽  
pp. 344-352 ◽  
Author(s):  
N.E. Redmond ◽  
R.W.M. van Soest ◽  
M. Kelly ◽  
J. Raleigh ◽  
S.A.A. Travers ◽  
...  
Keyword(s):  
18S Rrna ◽  
Gene Sequence ◽  
Sequence Data ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Gene Sequence Data

Leptospira broomii sp. nov., isolated from humans with leptospirosis

2006 ◽  
Vol 56 (3) ◽  
pp. 671-673 ◽  
Author(s):  
Paul N. Levett ◽  
Roger E. Morey ◽  
Renee L. Galloway ◽  
Arnold G. Steigerwalt
Keyword(s):  
16S Rrna ◽  
Gel Electrophoresis ◽  
Type Strain ◽  
Gene Sequence ◽  
Sequence Data ◽  
Pulsed Field Gel Electrophoresis ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Dna Relatedness ◽  
Gene Sequence Data

Isolates of Leptospira from two human cases of leptospirosis in Denmark and France were studied using DNA–DNA relatedness, G+C content, 16S rRNA gene sequence data and pulsed-field gel electrophoresis. These isolates differed from previously described species of Leptospira and are defined as Leptospira broomii sp. nov. The type strain is 5399T (=ATCC BAA-1107T=KIT 5399T).

scholarly journals Phylogeny of Twenty Thermus Isolates Constructed from 16S rRNA Gene Sequence Data

1993 ◽  
Vol 43 (4) ◽  
pp. 754-760 ◽  
Author(s):  
D. J. SAUL ◽  
A. G. RODRIGO ◽  
R. A. REEVES ◽  
L. C. WILLIAMS ◽  
K. M. BORGES ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Data ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Gene Sequence Data

scholarly journals Range extension of Ichthyophis multicolor Wilkinson et al., 2014 to India and first molecular identification of Ichthyophis moustakius Kamei et al., 2009

Check List ◽  
2021 ◽  
Vol 17 (4) ◽  
pp. 1021-1029
Author(s):  
Hmar Tlawmte Lalremsanga ◽  
Jayaditya Purkayastha ◽  
Mathipi Vabeiryureilai ◽  
Lal Muansanga ◽  
Ht Decemson ◽  
...  
Keyword(s):  
16S Rrna ◽  
Gene Sequence ◽  
Sequence Data ◽  
Northeast India ◽  
Range Extension ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Body Width ◽  
New Material ◽  
Gene Sequence Data

We report a substantial range extension of Ichthyophis multicolor Wilkinson, Presswell, Sherratt, Papadopoulou & Gower, 2014, with new material from Mizoram State, Northeast India. The species was previously known only from its type locality more than 800 km away in Ayeyarwady Region, Myanmar. The species was identified by both its morphology and 16s rRNA gene sequence data. One of the studied individuals represents the largest known specimen for the species (total length = 501 mm; mid-body width = 18.8 mm). Brief comparisons of I. multicolor with the sympatric as well as parapatric congeners in the region, and first barcode data for I. moustakius Kamei, Wilkinson, Gower & Biju, 2009 are also presented.

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