scholarly journals Application of Recombinant Human scFv Antibody as a Powerful Tool to Monitor Nitrogen Fixing Biofertilizer in Rice and Legume

2021 ◽  
Author(s):  
K. K. Khaing ◽  
K. Rangnoi ◽  
H. Michlits ◽  
N. Boonkerd ◽  
N. Teaumroong ◽  
...  
Keyword(s):  
Phage Display ◽  
Recombinant Antibodies ◽  
Scfv Antibody ◽  
Nitrogen Fixing ◽  
Phage Display Technology ◽  
Human Scfv ◽  
Display Technology

Human scFv antibody generated from phage display technology was successfully used for the generation of specific recombinant antibodies: yiN92-1e10 and yiDOA9-162 for the detection of Bradyrhizobium strains SUTN9-2 and DOA9, respectively.

scholarly journals Prokaryotic expression of MLAA-34 and generation of a novel human ScFv against MLAA-34 by phage display technology

Oncotarget ◽  
2017 ◽  
Vol 8 (24) ◽  
pp. 39077-39086
Author(s):  
Yang Zhang ◽  
Pengyu Zhang ◽  
Aili He ◽  
Yun Yang ◽  
Jianli Wang ◽  
...  
Keyword(s):  
Phage Display ◽  
Prokaryotic Expression ◽  
Phage Display Technology ◽  
Human Scfv ◽  
Display Technology

scholarly journals Isolation of a novel Anti-KDR3 Single-chain Variable Fragment Antibody from a Phage Display Library

2019 ◽  
Author(s):  
Shirafkan Kordi ◽  
Mohammad Rahmati-Yamchi ◽  
Mehdi Asghari Vostakolaei ◽  
Ali Etemadie ◽  
Abolfazl Barzegari ◽  
...  
Keyword(s):  
Phage Display ◽  
Growth Factor Receptor ◽  
Phage Display Library ◽  
Vital Role ◽  
Scfv Antibody ◽  
Single Chain Variable Fragment ◽  
Single Chain ◽  
Phage Display Technology ◽  
Display Technology ◽  
Domain 3

Vascular endothelial growth factor receptor 2 (VEGFR-2) is known as one of the important antigens playing a vital role in angiogenesis. In this study, phage display technology (PDT) was used to produce a single-chain variable fragment (scFv) antibody against a region of the domain 3 in VEGFR-2 called kinase insert domain receptor 3 (KDR3). After designing the KDR3 peptide and biopanning, a colony was chosen for scFv antibody expression. Following expression and purification; western blotting, dot blotting and immunofluorescence (IF) were used to evaluate the antibody function. Surface plasmon resonance (SPR) was also employed to measure affinity of produced antibody. Once a colony was selected and transferred to the expression host, the scFv antibody was expressed in the expected range of 28 kDa. Using a designed chromatography column, antibody purification was found to be about 95%. In this study, a novel scFv with the capability of binding to KDR3 was isolated and purified and its intracellular function was investigated and verified.

scholarly journals Mimotopes for Mycotoxins Diagnosis Based on Random Peptides or Recombinant Antibodies from Phage Library

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7652
Author(s):  
Wei Sun ◽  
Yan Zhang ◽  
Zhigang Ju
Keyword(s):  
Phage Display ◽  
Recombinant Antibody ◽  
Recombinant Antibodies ◽  
Phage Display Library ◽  
Ease Of Use ◽  
Phage Library ◽  
Phage Display Technology ◽  
Random Peptide ◽  
Display Technology

Mycotoxins, the small size secondary metabolites of fungi, have posed a threat to the safety of medicine, food and public health. Therefore, it is essential to create sensitive and effective determination of mycotoxins. Based on the special affinity between antibody and antigen, immunoassay has been proved to be a powerful technology for the detection of small analytes. However, the tedious preparation and instability of conventional antibodies restrict its application on easy and fast mycotoxins detection. By virtue of simplicity, ease of use, and lower cost, phage display library provides novel choices for antibodies or hapten conjugates, and lead random peptide or recombinant antibody to becoming the promising and environmental friendly immune-reagents in the next generation of immunoassays. This review briefly describes the latest developments on mycotoxins detection using M13 phage display, mainly focusing on the recent applications of phage display technology employed in mycotoxins detection, including the introduction of phage and phage display, the types of phage displayed peptide/recombinant antibody library, random peptides/recombinant antibodies-based immunoassays, as well as simultaneous determination of multiple mycotoxins.

Development of a Novel Human scFv Against EGFR L2 Domain by Phage Display Technology

2017 ◽  
Vol 23 (13) ◽  
pp. 2009-2014 ◽  
Author(s):  
Leila Rahbarnia ◽  
Safar Farajnia ◽  
Hossein Babaei ◽  
Jafar Majidi ◽  
Kamal Veisi ◽  
...  
Keyword(s):  
Phage Display ◽  
Phage Display Technology ◽  
Human Scfv ◽  
Display Technology

scholarly journals generation of hapten-specific recombinant antibodies: antibody phage display technology: a review

2012 ◽  
Vol 50 (No. 6) ◽  
pp. 231-252 ◽  
Author(s):  
J. Brichta ◽  
M. Hnilova ◽  
T. Viskovic
Keyword(s):  
Phage Display ◽  
Recombinant Dna ◽  
Recombinant Antibodies ◽  
Laboratory Animals ◽  
Automatic Process ◽  
Phage Display Technology ◽  
Current Trends ◽  
Antibody Phage ◽  
Antibody Phage Display ◽  
Display Technology

Production of antibodies has been revolutionized by the development of modern molecular biology methods for the expression of recombinant DNA. Phage display technology represents one of the most powerful tools for production and selection of recombinant antibodies and has been recognized as a valuable alternative way for the preparation of antibodies of a desired specificity. In comparison to poly- and monoclonal antibodies, recombinant antibodies using the phage display technology can be prepared faster, in more automatic process and with reduced consumption of laboratory animals. This review summarizes current trends of phage display technology with focus on the generation of hapten-specific recombinant antibodies and gives the examples of successful applications of phage display in the environmental analysis of low molecular weight compound.

Selection of Recombinant Antibodies by Phage Display Technology and Application for Detection of Allergenic Brazil Nut (Bertholletia excelsa) in Processed Foods

2013 ◽  
Vol 61 (43) ◽  
pp. 10310-10319 ◽  
Author(s):  
Silvia de la Cruz ◽  
Inés María López-Calleja ◽  
Marcos Alcocer ◽  
Isabel González ◽  
Rosario Martín ◽  
...  
Keyword(s):  
Phage Display ◽  
Recombinant Antibodies ◽  
Processed Foods ◽  
Brazil Nut ◽  
Phage Display Technology ◽  
Bertholletia Excelsa ◽  
Display Technology ◽  
Selection Of
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