UNDERSTANDING THE ROLE OF MICROBES IN THE FRESHWATER SEDIMENT CRYPTIC SULFUR CYCLE USING METAGENOMICS AND METATRANSCRIPTOMICS

2020 ◽  
Author(s):  
Emily Hyde ◽  
◽  
Cody Sheik ◽  
Sergei Katsev ◽  
Madelyn Petersen ◽  
...  
Keyword(s):  
Sulfur Cycle ◽  
Freshwater Sediment

The Influence of Benthic Biota on Copper Speciation and Distribution in Freshwater-Sediment Systems

1981 ◽  
Vol 16 (1) ◽  
pp. 45-58 ◽  
Author(s):  
G. Krantzberg ◽  
P.M. Stokes
Keyword(s):  
Freshwater Sediment ◽  
Benthic Macroinvertebrate ◽  
Macroinvertebrate Communities ◽  
Copper Species ◽  
Copper Speciation ◽  
Physico Chemical ◽  
Macro Benthos

Abstract An investigation was made of the effects exerted by benthic macroinvertebrate communities on copper speciation in sediments from a lake which is becoming acidified. In laboratory microcosms, benthic macroinvertebrate communities stimulated the flux of copper from sediment to water. The presence of the macro-benthos resulted in a redistribution of physico-chemical copper species within the sediment with a transfer from more strongly complexed forms (HC1 extractable) to adsorbed and cation exchangeable forms (MgCl2 extractable). The role of bio-turbation in copper transformations is discussed.

The role of the ocean in a regional sulfur cycle

1985 ◽  
Vol 90 (C5) ◽  
pp. 9168 ◽  
Author(s):  
Timothy S. Bates ◽  
Joel D. Cline
Keyword(s):  
Sulfur Cycle

scholarly journals The Ecological Role of a Proteolytic Psychrophile, Flavobacterium limicola Belonging to the Cytophaga-Flavobacteria Cluster, in the Decomposition of Particulate Organic Nitrogen in Freshwater Sediment

2004 ◽  
Vol 19 (2) ◽  
pp. 147-153 ◽  
Author(s):  
Hideyuki Tamaki ◽  
Satoshi Hanada ◽  
Kazunori Nakamura ◽  
Nakao Nomura ◽  
Masatoshi Matsumura ◽  
...  
Keyword(s):  
Organic Nitrogen ◽  
Freshwater Sediment ◽  
Ecological Role

scholarly journals Cryptic role of tetrathionate in the sulfur cycle: A study from Arabian Sea oxygen minimum zone sediments

2019 ◽  
Author(s):  
Subhrangshu Mandal ◽  
Sabyasachi Bhattacharya ◽  
Chayan Roy ◽  
Moidu Jameela Rameez ◽  
Jagannath Sarkar ◽  
...  
Keyword(s):  
Arabian Sea ◽  
Potential Role ◽  
Sediment Cores ◽  
Oxygen Minimum Zone ◽  
Sulfur Cycle ◽  
Metagenome Analysis ◽  
Minimum Zone ◽  
Oxygen Minimum

ABSTRACTTo explore the potential role of tetrathionate in the sulfur cycle of marine sediments, the population ecology of tetrathionate-forming, oxidizing, and respiring microorganisms was revealed at 15-30 cm resolution along two, ∼3-m-long, cores collected from 530- and 580-mbsl water-depths of Arabian Sea, off India’s west coast, within the oxygen minimum zone (OMZ). Metagenome analysis along the two sediment-cores revealed widespread occurrence of the structural genes that govern these metabolisms; high diversity and relative-abundance was also detected for the bacteria known to render these processes. Slurry-incubation of the sediment-samples, pure-culture isolation, and metatranscriptome analysis, corroborated thein situfunctionality of all the three metabolic-types. Geochemical analyses revealed thiosulfate (0-11.1 μM), pyrite (0.05-1.09 wt %), iron (9232-17234 ppm) and manganese (71-172 ppm) along the two sediment-cores. Pyrites (via abiotic reaction with MnO2) and thiosulfate (via oxidation by chemolithotrophic bacteria prevalentin situ) are apparently the main sources of tetrathionate in this ecosystem. Tetrathionate, in turn, can be either converted to sulfate (via oxidation by the chemolithotrophs present) or reduced back to thiosulfate (via respiration by native bacteria); 0-2.01 mM sulfide present in the sediment-cores may also reduce tetrathionate abiotically to thiosulfate and elemental sulfur. Notably tetrathionate was not detectedin situ- high microbiological and geochemical reactivity of this polythionate is apparently instrumental in the cryptic nature of its potential role as a central sulfur cycle intermediate. Biogeochemical roles of this polythionate, albeit revealed here in the context of OMZ sediments, may well extend to the sulfur cycles of other geomicrobiologically-distinct marine sediment horizons.

scholarly journals Cryptic role of tetrathionate in the sulfur cycle: A study from Arabian Sea oxygen minimum zone sediments

2019 ◽  
Author(s):  
Subhrangshu Mandal ◽  
Sabyasachi Bhattacharya ◽  
Chayan Roy ◽  
Moidu Jameela Rameez ◽  
Jagannath Sarkar ◽  
...  
Keyword(s):  
Arabian Sea ◽  
Potential Role ◽  
Sediment Cores ◽  
Oxygen Minimum Zone ◽  
Sulfur Cycle ◽  
Metagenome Analysis ◽  
Minimum Zone ◽  
Oxygen Minimum

Abstract. To explore the potential role of tetrathionate in the sulfur cycle of marine sediments, the population ecology of tetrathionate-forming, oxidizing, and respiring microorganisms was revealed at 15–30 cm resolution along two, ~ 3-m-long, cores collected from 530- and 580-mbsl water-depths of Arabian Sea, off India’s west coast, within the oxygen minimum zone (OMZ). Metagenome analysis along the two sediment-cores revealed widespread occurrence of the structural genes that govern these metabolisms; high diversity and relative-abundance was also detected for the bacteria known to render these processes. Slurry-incubation of the sediment-samples, pure-culture isolation, and metatranscriptome analysis, corroborated the in situ functionality of all the three metabolic-types. Geochemical analyses revealed thiosulfate (0–11.1 µM), pyrite (0.05–1.09 wt %), iron (9232–17234 ppm) and manganese (71–172 ppm) along the two sediment-cores. Pyrites (via abiotic reaction with MnO2) and thiosulfate (via oxidation by chemolithotrophic bacteria prevalent in situ) are apparently the main sources of tetrathionate in this ecosystem. Tetrathionate, in turn, can be either converted to sulfate (via oxidation by the chemolithotrophs present) or reduced back to thiosulfate (via respiration by native bacteria); 0–2.01 mM sulfide present in the sediment-cores may also reduce tetrathionate abiotically to thiosulfate and elemental sulfur. Notably tetrathionate was not detected in situ – high microbiological and geochemical reactivity of this polythionate is apparently instrumental in the cryptic nature of its potential role as a central sulfur cycle intermediate. Biogeochemical roles of this polythionate, albeit revealed here in the context of OMZ sediments, may well extend to the sulfur cycles of other geomicrobiologically-distinct marine sediment horizons.

scholarly journals Comparative genomics: Dominant coral-bacterium Endozoicomonas acroporae metabolizes dimethylsulfoniopropionate (DMSP)

2019 ◽  
Author(s):  
Kshitij Tandon ◽  
Pei-Wen Chiang ◽  
Chih-Ying Lu ◽  
Naohisa Wada ◽  
Shan-Hua Yang ◽  
...  
Keyword(s):  
Sole Carbon Source ◽  
Genomic Analysis ◽  
Sulfur Cycle ◽  
Comparative Genomic Analysis ◽  
Comparative Genomic ◽  
High Quality ◽  
Coa Transferase ◽  
Complete Genomes ◽  
Central Carbon

AbstractDominant coral-associated Endozoicomonas bacteria species are hypothesized to play a role in the coral-sulfur cycle by metabolizing Dimethylsulfoniopropionate (DMSP) into Dimethylsulfide (DMS); however, no sequenced genome to date harbors genes for this process. In this study, we assembled high-quality (>95% complete) genomes of strains of a recently added species Endozoicomonas acroporae (Acr-14T, Acr-1 and Acr-5) isolated from the coral Acropora muricata and performed comparative genomic analysis on genus Endozoicomonas. We identified the first DMSP CoA-transferase/lyase—a dddD gene homolog found in all E. acroporae strains—and functionally characterized bacteria capable of metabolizing DMSP into DMS via the DddD cleavage pathway using RT-qPCR and gas chromatography (GC). Furthermore, we demonstrated that E. acroporae strains can use DMSP as the sole carbon source and have genes arranged in an operon-like manner to link DMSP metabolism to the central carbon cycle. This study confirms the role of Endozoicomonas in the coral sulfur cycle.

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