On the role of spectral diffusion in single-molecule spectroscopy

2000 ◽  
Vol 42 (3) ◽  
pp. 477-480 ◽  
Author(s):  
V. V. Palm ◽  
K. K. Rebane ◽  
A. Zabrodskii
Keyword(s):  
Single Molecule ◽  
Spectral Diffusion ◽  
Single Molecule Spectroscopy

Role of rare sites in single molecule spectroscopy measurements of spectral diffusion

2001 ◽  
Vol 114 (23) ◽  
pp. 10479-10485 ◽  
Author(s):  
Douglas S. English ◽  
Elizabeth J. Harbron ◽  
Paul F. Barbara
Keyword(s):  
Single Molecule ◽  
Spectral Diffusion ◽  
Single Molecule Spectroscopy

scholarly journals Stability of a Nonequilibrium Biochemical Cycle Revealed by Single-Molecule Spectroscopy

2021 ◽  
Author(s):  
Saurabh Talele ◽  
John T King
Keyword(s):  
Entropy Production ◽  
Single Molecule ◽  
Nonequilibrium Dynamics ◽  
Single Molecule Spectroscopy ◽  
Reaction Cycle ◽  
Central Interest ◽  
Thermodynamic Driving Force ◽  
Reaction Cycles ◽  
Thermal Stability Of

Biological machinery relies on nonequilibrium dynamics to maintain stable directional fluxes through complex reaction cycles. In stabilizing the reaction cycle, the role of microscopic irreversibility of elementary transitions, and the accompanying entropy production, is of central interest. Here, we use multidimensional single-molecule spectroscopy to demonstrate that the reaction cycle of bacteriorhodopsin is coupled through both reversible and irreversible transitions, with directionality of trans-membrane H+ transport being ensured by the entropy production of irreversible transitions. We observe that thermal destabilization of the process is the result of diminishing thermodynamic driving force for irreversible transitions, leading to an exponentially increasing variance of flux through the transitions. We show that the thermal stability of the reaction cycle can be predicted from the Gibbs-Helmholtz relation.

scholarly journals Role of Denatured-State Properties in Chaperonin Action Probed by Single-Molecule Spectroscopy

2014 ◽  
Vol 107 (12) ◽  
pp. 2891-2902 ◽  
Author(s):  
Hagen Hofmann ◽  
Frank Hillger ◽  
Cyrille Delley ◽  
Armin Hoffmann ◽  
Shawn H. Pfeil ◽  
...  
Keyword(s):  
Single Molecule ◽  
Single Molecule Spectroscopy ◽  
Denatured State

Spectral diffusion in an amorphous polymer probed by single molecule spectroscopy

1995 ◽  
Vol 64 (1-6) ◽  
pp. 1-11 ◽  
Author(s):  
J. Tittel ◽  
R. Kettner ◽  
Th. Basché ◽  
C. Bräuchle ◽  
H. Quante ◽  
...  
Keyword(s):  
Single Molecule ◽  
Amorphous Polymer ◽  
Spectral Diffusion ◽  
Single Molecule Spectroscopy

Spectral diffusion of the lowest exciton component in the core complex from Rhodopseudomonas palustris studied by single-molecule spectroscopy

2007 ◽  
Vol 95 (2-3) ◽  
pp. 285-290 ◽  
Author(s):  
Martin F. Richter ◽  
Jürgen Baier ◽  
June Southall ◽  
Richard J. Cogdell ◽  
Silke Oellerich ◽  
...  
Keyword(s):  
Single Molecule ◽  
Rhodopseudomonas Palustris ◽  
Spectral Diffusion ◽  
Single Molecule Spectroscopy ◽  
Core Complex ◽  
The Core

SINGLE MOLECULE SPECTROSCOPY

1991 ◽  
Vol 05 (11) ◽  
pp. 747-751 ◽  
Author(s):  
M. ORRIT ◽  
J. BERNARD
Keyword(s):  
Single Molecule ◽  
Optical Detection ◽  
Local Environment ◽  
Spectral Diffusion ◽  
Single Molecule Spectroscopy ◽  
Optical Study ◽  
Temperature Changes ◽  
Relative Shift ◽  
Resonance Frequencies ◽  
Molecular Resonance

We report unambiguous optical detection of single molecules of pentacene in p-terphenyl crystal. The relative shift of the molecular resonance frequencies with temperature changes demonstrates the phenomenon of activated spectral diffusion. These results show the feasibility of the optical study of a single molecule and its local environment.

Sign in / Sign up

Export Citation Format

Share Document