Measurements of the phonon spectrum of the LED heterostructure based on the In0.12Ga0.88N/GaN barrier showed the presence in it of four peaks of phonon radiation with energies of 0.193, 0.207, 0.353, and 0.356 eV. From a comparison of the results of the calculation of the energy spectrum of the electron and hole quantum wells with the obtained experimental data, it was assumed that these peaks can be interpreted as the energies of phonons generated during the capture of electrons from the barrier layer to the second level of dimensional quantization, as well as during the relaxation of electrons from the second level to the radiation level and trapping holes to the upper level of the quantum well.