Efficiencies of introduction of an oil-oxidizing Dietzia maris strain and stimulation of natural microbial communities in remediation of polluted soil

Abstract. Soils are increasingly threatened by spillage of petroleum products such as petrol, diesel fuel, gasoline at oil refineries, underground storage tanks and pump stations pipelines. This study aims to investigate the effectiveness of Pseudomonas putida as oil-biodegradable agent in soil contaminated with diesel (D100). The effectiveness on bioremediation have been conducted by examined several physico-chemical tests on diesel-contaminated soil before and after seeding with P. putida. The spillage stimulation of D100 was conducted at laboratory scale for 24 days of incubation time. The results show that the bioremediation treatment able to remove up to 82%, 55%, 48%, and 34% of nitrogen, total organic carbon, phosphate, sulfate and, respectively. The pH of soil sample was changed from pH 7.8 (Day 0) to 6.78 (Day 24) after the treatment. Meanwhile, the moisture content in the sample has increased from 39% (Day 0) to 59% (Day 24). All of these results show the good indication of quality improvement of polluted soil after treated with P. putida. It is apparent from the acquired results that the application of P. putida is suitable as effective microorganism and potentially exploits as useful diesel-soil biodegradable agent in polluted soil.

Download Full-text

Bioremediation of Biodiesel/Diesel Blend (B50) in Soil Contamination by Using Pseudomonas putida

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.699.1012 ◽

2014 ◽

Vol 699 ◽

pp. 1012-1019 ◽

Cited By ~ 1

Author(s):

N.M. Sunar ◽

Q. Emparan ◽

Ahmad Tarmizi Abdul Karim ◽

M. Maslan ◽

F. Mustafa ◽

...

Keyword(s):

Quality Improvement ◽

Organic Carbon ◽

Moisture Content ◽

Pseudomonas Putida ◽

Polluted Soil ◽

Physico Chemical ◽

Before And After ◽

Biodiesel Fuels ◽

Effective Microorganism ◽

Stimulation Of

The biodiesel fuels nowadays are attracting worldwide attention as blending components or direct replacements for diesel fuel in conventional diesel engines. This study aims to investigate the effectiveness ofPseudomonas putidaas oil-biodegradable agent in soil contaminated with biodiesel/diesel blends (B50). The effectiveness on bioremediation has been conducted by examining several physico-chemical tests on biodiesel/diesel-contaminated soil before and after seeding ofPseudomonasputida. The spillage stimulation of B50 was conducted at laboratory scale for 24 days of incubation time. The results show that the bioremediation treatment able to remove up to 82%, 54%, 38% and 10% of nitrogen, sulfate, phosphate and organic carbon, respectively. The pH of soil sample was changed from pH 7.42 (Day 0) to pH 6.38 (Day 24) after the treatment. Meanwhile, the moisture content in the sample has increased from 25.61% (Day 0) to 31.83% (Day 24). All results show the good indication of quality improvement for polluted soil after treated withPseudomonasputida. It is apparent from the acquired results that the application ofPseudomonasputidais suitable as effective microorganism and potentially useful as oil-soil biodegradable agent in polluted soil.

Download Full-text

Adaptation of microbial communities in soil contaminated with polychlorinated biphenyls, leading to the transformation of more highly chlorinated congeners in biofilm communities

Biofilms ◽

10.1017/s1479050507002116 ◽

2006 ◽

Vol 3 (1) ◽

pp. 37-46 ◽

Cited By ~ 8

Author(s):

A. J. Macedo ◽

T. R. Neu ◽

U. Kuhlicke ◽

W.-R. Abraham

Keyword(s):

Polychlorinated Biphenyls ◽

Microbial Communities ◽

Contaminated Soil ◽

Polluted Soil ◽

Soil Samples ◽

Metabolic Adaptation ◽

Strong Increase ◽

Pcb Congeners ◽

A Site

ABSTRACTA site polluted for many years with polychlorinated biphenyls (PCB) was used to elucidate the metabolic adaptation of microbial communities to these xenobiotics. Soil samples taken along a gradient of PCB-pollution at this site were used to grow biofilm communities on PCB oil. The biofilm communities originating from the non-polluted soil formed rather uniform and thin bacterial layers on PCB oil, while the biofilms originating from contaminated soil samples formed agglomerated structures on the PCB droplets. Biofilm communities were very diverse but those from highly polluted soil were dominated byBurkholderiaspecies, a genus known for degrading several PCBs. All biofilm communities could transform low to medium chlorinated PCB congeners but a strong increase in the rate and degree of PCB transformation in communities from heavily polluted soil was observed. Notably, pentachlorinated congeners were transformed only by biofilms derived from the highly polluted soil but at the same time the content of trichlorinated congeners did not decrease. It is assumed that biofilms from the highly contaminated soil reductively dechlorinated PCB, converting pentachlorinated congeners to trichlorinated congeners in the spherical biofilm aggregates by diffusing to the surface of the aggregates, where aerobic transformation took place.

Download Full-text

Distinct Responses of Rare and Abundant Microbial Taxa to In Situ Chemical Stabilization of Cadmium-Contaminated Soil

mSystems ◽

10.1128/msystems.01040-21 ◽

2021 ◽

Author(s):

Min Xu ◽

Qiaoyun Huang ◽

Zhenqian Xiong ◽

Hao Liao ◽

Zhenguang Lv ◽

...

Keyword(s):

Heavy Metal ◽

Microbial Communities ◽

Abundant Species ◽

Polluted Soil ◽

Ecosystem Functions ◽

Chemical Stabilization ◽

Soil Ecosystem ◽

Ecosystem Multifunctionality ◽

Cadmium Contaminated Soil

Understanding the ecological roles of rare and abundant species in the restoration of soil ecosystem functions is crucial to remediation of heavy metal-polluted soil. Our study assessed the efficiencies of five commonly used soil amendments on recovery of ecosystem multifunctionality and emphasized the relative contributions of rare and abundant microbial communities to ecosystem multifunctionality.

Download Full-text

Contrasting Effects of Environmental Concentrations of Sulfonamides on Microbial Heterotrophic Activities in Freshwater Sediments

Frontiers in Microbiology ◽

10.3389/fmicb.2021.753647 ◽

2021 ◽

Vol 12 ◽

Author(s):

Stéphane Pesce ◽

Laura Kergoat ◽

Laurianne Paris ◽

Loren Billet ◽

Pascale Besse-Hoggan ◽

...

Keyword(s):

Microbial Communities ◽

Surface Sediments ◽

Temporal Dynamics ◽

River Sediments ◽

Transient Effects ◽

High Concentration ◽

Environmental Concentrations ◽

Natural Stream ◽

Measured Activity ◽

Stimulation Of

The sulfonamide antibiotics sulfamethoxazole (SMX) and sulfamethazine (SMZ) are regularly detected in surface sediments of contaminated hydrosystems, with maximum concentrations that can reach tens of μg kg–1 in stream and river sediments. Little is known about the resulting effects on the exposed benthic organisms. Here we investigated the functional response of stream sediment microbial communities exposed for 4 weeks to two levels of environmentally relevant concentrations of SMX and SMZ, tested individually. To this end, we developed a laboratory channel experiment where natural stream sediments were immersed in water contaminated with nominal environmental concentrations of 500 and 5,000 ng L–1 of SMX or SMZ, causing their accumulation in surface sediments. The mean maximum concentrations measured in the sediment (about 2.1 μg SMX kg–1 dw and 4.5 μg SMZ kg–1 dw) were consistent with those reported in contaminated rivers. The resulting chronic exposure had various effects on the functional potential of the sediment microbial communities, according to the substance (SMX or SMZ), the type of treatment (high or low) and the measured activity, with a strong influence of temporal dynamics. Whereas the SMZ treatments resulted in only transient effects on the five microbial activities investigated, we observed a significant stimulation of the β-glucosidase activity over the 28 days in the communities exposed to the high concentration of SMX. Together with the stimulation of aerobic respiration at low SMX concentrations and the reduced concentration observed in the last days, our results suggest a potential biodegradation of sulfonamides by microbial communities from sediments. Given the key functional role of surface sediment microbial communities in streams and rivers, our findings suggest that the frequently reported contamination of sediments by sulfonamides is likely to affect biogeochemical cycles, with possible impact on ecosystem functioning.

Download Full-text

Bioremediation of Biofuel-Soil Contamination by Using Pseudomonas putida

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.845.138 ◽

2013 ◽

Vol 845 ◽

pp. 138-145 ◽

Cited By ~ 8

Author(s):

N.M. Sunar ◽

Quin Emparan ◽

Ahmad Tarmizi Abdul Karim ◽

S.F.M. Noor ◽

M. Maslan ◽

...

Keyword(s):

Quality Improvement ◽

Organic Carbon ◽

Moisture Content ◽

Pseudomonas Putida ◽

Polluted Soil ◽

Physico Chemical ◽

Environmental Damages ◽

Before And After ◽

Effective Microorganism ◽

Stimulation Of

Abstract. The commercialization of biodiesel/diesel blends on the market can cause environmental damages due to spills. This study aims to investigate the effectiveness ofPseudomonas putidaas oil-biodegradable agent in soil contaminated with biodiesel/diesel blend (B20). The effectiveness on bioremediation have been conducted by examined several physico-chemical tests on biodiesel/diesel-contaminated soil before and after seeding ofP. putida. The spillage stimulation of B20 was conducted at laboratory scale for 24 days of incubation time. The results show that the bioremediation treatment able to remove up to 82%, 77%, 16%, and 10% of nitrogen, phosphate, sulfate and total organic carbon, respectively. The pH of soil sample was changed from pH 7.45 (Day 0) to 7.25 (Day 24) after the treatment. Meanwhile, the moisture content in the sample has increased from 44.11% (Day 0) to 50.35% (Day 24). All of these results show the good indication of quality improvement of polluted soil after treated withP. putida. It is apparent from the acquired results that the application ofP. putidais suitable as effective microorganism and potentially exploits as useful oil-soil biodegradable agent in polluted soil.

Download Full-text