Immunochromatographic assay for serodiagnosis of tuberculosis using an antigen–colloidal gold conjugate

2015 ◽  
Vol 51 (8) ◽  
pp. 834-839 ◽  
Author(s):  
D. V. Sotnikov ◽  
A. V. Zherdev ◽  
V. G. Avdienko ◽  
B. B. Dzantiev
Keyword(s):  
Colloidal Gold ◽  
Immunochromatographic Assay ◽  
Gold Conjugate

scholarly journals Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 365
Author(s):  
Fengli Liu ◽  
Yanxin Cao ◽  
Maokai Yan ◽  
Mengxu Sun ◽  
Qingshui Zhang ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Colloidal Gold ◽  
Preventive Intervention ◽  
Virus Detection ◽  
Cross Reactivity ◽  
Duck Enteritis Virus ◽  
Detection Sensitivity ◽  
Immunochromatographic Assay ◽  
Efficient Detection ◽  
Enteritis Virus

Duck viral enteritis is a highly contagious and fatal disease of commercial waterfowl flocks. The disease occurs sporadically or epizootically in mainland China due to insufficient vaccinations. Early and rapid diagnosis is important for preventive intervention and the control of epizootic events in clinical settings. In this study, we generated two monoclonal antibodies (MAbs) that specifically recognized the duck enteritis virus (DEV) envelope glycoprotein B and tegument protein UL47, respectively. Using these MAbs, a colloidal gold-based immunochromatographic assay (ICA) was developed for the efficient detection of DEV antigens within 15 min. Our results showed that the detection limit of the developed ICA strip was 2.52 × 103 TCID50/mL for the virus infected cell culture suspension with no cross-reactivity with other pathogenic viruses commonly encountered in commercially raised waterfowl. Using samples from experimentally infected ducks, we demonstrated that the ICA detected the virus in cloacal swab samples on day three post-infection, demonstrating an 80% concordance with the PCR. For tissue homogenates from ducks succumbing to infection, the detection sensitivity was 100%. The efficient and specific detection by this ICA test provides a valuable, convenient, easy to use and rapid diagnostic tool for DVE under both laboratory and field conditions.

scholarly journals Development of Immunochromatographic Assay for the Rapid Detection of Mycoplasma capricolum subsp. capripneumoniae Antibodies

2022 ◽  
Vol 12 ◽  
Author(s):  
Zhen Zhu ◽  
Guanggang Qu ◽  
Changjiang Wang ◽  
Lei Wang ◽  
Jige Du ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Colloidal Gold ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Immunochromatographic Assay ◽  
Economic Losses ◽  
Serum Samples ◽  
Mycoplasma Capricolum ◽  
Prokaryotic Expression System

Mycoplasma capricolum subsp. capripneumoniae (Mccp) is the cause of contagious caprine pleuropneumonia (CCPP), which is a highly significant respiratory disease in goats leading to significant economic losses in Africa and Asia. Currently available procedures for the diagnosis of CCPP have some limitations in sensitivity, specificity, operation time, requirement of sophisticated equipment or skilled personnel, and cost. In this study, we developed a rapid, sensitive, and specific colloidal gold-based immunochromatographic assay (GICA) strip for the efficient on-site detection of antibodies against Mccp in the serum within 10 min. For the preparation of this colloidal GICA strip, recombinant P20 protein, the membrane protein of Mccp, was expressed by Escherichia coli prokaryotic expression system after purification was used as the binding antigen in the test. The rabbit anti-goat immunoglobulin G labeled with the colloidal gold was used as the detection probe, whereas the goat anti-rabbit immunoglobulin G was coated on the nitrocellulose membrane as the control line. The concentration of the coating antibody was optimized, and the effectiveness of this colloidal GICA strip was evaluated. Our results proved that the detection limit of the test strip was up to 1:64 dilutions for the Mccp antibody-positive serum samples with no cross-reactivity with other pathogens commonly infecting small ruminants,including goat pox virus, peste des petits ruminants virus, foot-and-mouth disease virus type A, or other mycoplasmas. Moreover, the colloidal GICA strip was more sensitive and specific than the indirect hemagglutination assay for the detection of Mccp antibodies. The 106 clinical serum samples were detected by the colloidal GICA strip compared with the complement fixation test, demonstrating an 87.74% concordance with the complement fixation test. This novel colloidal GICA strip would be an effective tool for the cost-effective and rapid diagnosis of CCPP in the field.

The allocation of cells in the presomitic mesoderm during somite segmentation in the mouse embryo

Development ◽  
1988 ◽  
Vol 103 (2) ◽  
pp. 379-390 ◽  
Author(s):  
P.P. Tam
Keyword(s):  
Mouse Embryo ◽  
Colloidal Gold ◽  
Wheat Germ ◽  
Mouse Embryos ◽  
Middle Region ◽  
Presomitic Mesoderm ◽  
Posterior Displacement ◽  
Somite Formation ◽  
Gold Conjugate

Orthotopic grafts of wheat germ agglutinin-colloidal gold conjugate (WGA-gold) labelled cells were used to demonstrate differences in the segmental fate of cells in the presomitic mesoderm of the early-somite-stage mouse embryos developing in vitro. Labelled cells in the anterior region of the presomitic mesoderm colonized the first three somites formed after grafting, while those grafted to the middle region of this tissue were found mostly in the 4th-7th newly formed somites. Labelled cells grafted to the posterior region were incorporated into somites whose somitomeres were not yet present in the presomitic mesoderm at the time of grafting. There was therefore an apparent posterior displacement of the grafted cells in the presomitic mesoderm. Colonization of somites by WGA-gold labelled cells was usually limited to two to three consecutive somites in the chimaera. The distribution of cells derived from a single graft to two somites was most likely due to the segregation of the labelled population when cells were allocated to adjacent meristic units during somite formation. Further spreading of the labelled cells to several somites in some cases was probably the result of a more extensive mixing of mesodermal cells among the somitomeres prior to somite segmentation.

scholarly journals Development of Colloidal Gold-Based Immunochromatographic Assay for Rapid Detection of Goose Parvovirus

2018 ◽  
Vol 9 ◽  
Author(s):  
Xianglong Yu ◽  
Lei Wei ◽  
Hao Chen ◽  
Xiaoyu Niu ◽  
Yanguo Dou ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
Rapid Detection ◽  
Immunochromatographic Assay ◽  
Goose Parvovirus

Matrix effect of five kinds of meat on colloidal gold immunochromatographic assay for sulfamethazine detection

2018 ◽  
Vol 10 (37) ◽  
pp. 4505-4510 ◽  
Author(s):  
Namei Liu ◽  
Keyu Xing ◽  
Chun Wang ◽  
Ganggang Zhang ◽  
Meifang Yuan ◽  
...  
Keyword(s):  
Matrix Effect ◽  
Colloidal Gold ◽  
Immunochromatographic Assay

A colloidal gold immunochromatographic assay (CG-ICA) was developed to detect sulfamethazine (SM2) residues in five kinds of meat: namely, chicken, pork, shrimp, beef, and fish.

A colloidal gold probe-based silver enhancement immunochromatographic assay for the rapid detection of abrin-a

2011 ◽  
Vol 26 (8) ◽  
pp. 3710-3713 ◽  
Author(s):  
Wei Yang ◽  
Xiao-bing Li ◽  
Guo-wen Liu ◽  
Bing-bing Zhang ◽  
Yi Zhang ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
Rapid Detection ◽  
Immunochromatographic Assay ◽  
Silver Enhancement ◽  
Gold Probe
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