Influence of Mono- and Oligosaccharides on cbh1 Gene Transcription in the Filamentous Fungus Penicillium verruculosum

2021 ◽  
Vol 57 (9) ◽  
pp. 925-932
Author(s):  
V. Yu. Kislitsin ◽  
A. M. Chulkin ◽  
I. N. Zorov ◽  
I. A. Shashkov ◽  
A. D. Satrutdinov ◽  
...  
Keyword(s):  
Gene Transcription ◽  
Filamentous Fungus ◽  
Penicillium Verruculosum

Influence of Mono- and Oligosaccharides on the cbh1 Gene Transcripion in the Penicillium verruculosum Filamentous Fungus

2021 ◽  
Vol 37 (1) ◽  
pp. 45-53
Author(s):  
A.M. Chulkin ◽  
I.N. Zorov ◽  
I.A. Shashkov ◽  
A.D. Satrutdinov ◽  
A.P. Sinitsyn ◽  
...  
Keyword(s):  
Carbon Source ◽  
Gene Transcription ◽  
Filamentous Fungus ◽  
Basic Research ◽  
Degree Of Polymerization ◽  
Penicillium Verruculosum ◽  
Rt Pcr ◽  
Catabolic Repression ◽  
Rfbr Grant ◽  
First Time

A method has been developed for the analysis of the induction of the cbh1 gene transcription in the filamentous fungus Penicillium verruculosum after treatment with mono- and oligosaccharides. The method allows obtaining mRNA, which encodes cellobiohydrolase-1 (CBH1) in an amount sufficient for RT-PCR. Citrate, a compound that does not cause carbon catabolic repression, was used as a carbon source. It was shown for the first time that xylose, gentiobiose, and mainly cellobiose induce the expression of the cbh1 gene in P. verruculosum, while sophorose and a mixture of xylooligosaccharides with a degree of polymerization from 3 to 5 are most likely to serve as precursors of inducers. Penicillium verruculosum, qPCR, RT-PCR, monosaccharides, oligosaccharides, inducer The work was supported by the Russian Foundation for Basic Research (RFBR) (grant no.18-29-07070).

Expression of CAS9 Nuclease of the CRISPR/Cas Genome Editing System in the Filamentous Fungus Penicillium verruculosum

2020 ◽  
Vol 75 (4) ◽  
pp. 243-249
Author(s):  
V. Yu. Kislitsin ◽  
A. M. Chulkin ◽  
I. G. Sinelnikov ◽  
A. P. Sinitsyn ◽  
A. M. Rozhkova
Keyword(s):  
Genome Editing ◽  
Filamentous Fungus ◽  
Penicillium Verruculosum ◽  
Cas9 Nuclease

Structural investigation of endoglucanase 2 from the filamentous fungus Penicillium verruculosum

2017 ◽  
Vol 62 (2) ◽  
pp. 254-259
Author(s):  
A. V. Vakhrusheva ◽  
V. A. Nemashkalov ◽  
O. V. Kravchenko ◽  
S. V. Tishchenko ◽  
A. G. Gabdulkhakov ◽  
...  
Keyword(s):  
Filamentous Fungus ◽  
Structural Investigation ◽  
Penicillium Verruculosum

scholarly journals Genome Editing in Filamentous Fungus Penicillium Verruculosum Using the CRISPR/Cas9 System

2021 ◽  
Author(s):  
Valeriy Kislitsin ◽  
Andrey Chulkin ◽  
Ivan Zorov ◽  
Yuri Denisenko ◽  
Arkadiy Sinitsyn Sinitsyn ◽  
...  
Keyword(s):  
Nitrate Reductase ◽  
Genome Editing ◽  
Filamentous Fungus ◽  
Penicillium Verruculosum ◽  
Selective Marker ◽  
Double Knockout ◽  
Nitrate Reductase Gene ◽  
Reductase Gene ◽  
Industrial Strains ◽  
First Time

Abstract PurposeTo adapt CRISPR/Cas9 genome editing method for use in filamentous fungus Penicillium verruculosum, which is industrial producer of carbohydrases. ResultsFor the first time the CRISPR/Cas9 method was adapted for genome editing in the filamentous fungi Penicillium verrucullosum. Using the nitrate reductase gene (niaD) as a selective marker with the CRISPR/Cas9 system we performed double knockout of niaD and cellobiohydrolase 1 (cbh1) genes. The efficiency of double editing was 50%. At the same time, it was unexpected that the specific cellobiohydrolase activity rised after knockout of cbh1 gene due to the increase CBH2 expression. ConclusionWe developed effective method for genome editing in P. verruculosum, which can be used to improve qualities of industrial strains.

Activator protein-1 is necessary for angiotensin-II stimulation of human adrenocorticotropin receptor gene transcription

2001 ◽  
Vol 268 (6) ◽  
pp. 1802-1810
Author(s):  
Danielle Naville ◽  
Estelle Bordet ◽  
Marie-Claude Berthelon ◽  
Philippe Durand ◽  
Martine Begeot
Keyword(s):  
Angiotensin Ii ◽  
Gene Transcription ◽  
Receptor Gene ◽  
Activator Protein 1 ◽  
Activator Protein ◽  
Stimulation Of

Regulation of neuronal cholecystokinin gene transcription

2001 ◽  
Vol 61 (7) ◽  
pp. 61-67 ◽  
Author(s):  
Thomas V. O. Hansen ◽  
Finn C. Nielsen
Keyword(s):  
Gene Transcription

Q-mediated late gene transcription of bacteriophage λ: RNA start point and RNase III processing sites in vivo

Virology ◽  
1988 ◽  
Vol 167 (2) ◽  
pp. 568-577 ◽  
Author(s):  
D DANIELS ◽  
M SUBBARAO ◽  
F BLATTNER ◽  
H LOZERON
Keyword(s):  
Gene Transcription ◽  
Late Gene ◽  
Bacteriophage Λ ◽  
Rnase Iii

GENE ACTIVATION IN MAMMARY CELLS

1972 ◽  
Vol 71 (2_Suppla) ◽  
pp. S346-S368 ◽  
Author(s):  
Roger W. Turkington ◽  
Nobuyuki Kadohama
Keyword(s):  
Cell Differentiation ◽  
Gene Transcription ◽  
Hormonal Regulation ◽  
Gene Activation ◽  
Milk Proteins ◽  
Mouse Mammary Gland ◽  
Nuclear Proteins ◽  
Mammary Cells ◽  
Alveolar Cells ◽  
Mammary Cell

ABSTRACT Hormonal activation of gene transcription has been studied in a model system, the mouse mammary gland in organ culture. Transcriptive activity is stimulated in mammary stem cells by insulin, and in mammary alveolar cells by prolactin and insulin. Studies on the template requirement for expression of the genes for milk proteins demonstrate that DNA methylation has an obligatory dependence upon DNA synthesis, but is otherwise independent from hormonal regulation of mammary cell differentiation. Incorporation of 5-bromo-2′deoxyuridine into DNA selectively inhibits expression of the genes for specific milk proteins. Undifferentiated mammary cells activate the synthesis of specific acidic nuclear proteins when stimulated by insulin. Several of these induced acidic nuclear proteins are undetectable in unstimulated undifferentiated cells, but appear to be characteristic components of the nuclei of differentiated cells. These results indicate that mammary cell differentiation is associated with a change in acidic nuclear proteins, and they provide evidence to support the concept that acidic nuclear proteins may be involved in the regulation of gene transcription and of mammary cell differentiation.

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