Recovery of acetylcholine-induced currents after paired-pulse stimulation in snail neurons: Analysis using a mathematical model

1. The excitation of lumbar motoneurons by reticulospinal axons traveling in the medial longitudinal fasciculus (MLF) was investigated in the newborn rat using intracellular recordings from lumbar motoneurons in an in vitro preparation of the brain stem and spinal cord. The tracer DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine) was introduced into the MLF of 6-day-old littermate rats that had been fixed with paraformaldehyde to evaluate the anatomic extent of this developing pathway. 2. Fibers labeled from the MLF by DiI were present in the cervical ventral and lateral white matter and a smaller number of labeled fibers extended to the lumbar enlargement. Patches of sparse terminal labeling were seen in the lumbar ventral gray. 3. In the in vitro preparation of the brain stem and spinal cord, MLF stimulation excited motoneurons through long-latency pathways in most motoneurons and through both short-(< 40 ms) and long-latency connections in 16 of 40 motoneurons studied. Short- and longer-latency components of the excitatory response were evaluated using mephenesin to reduce activity in polysynaptic pathways. 4. Paired-pulse stimulation of the MLF revealed a modest temporal facilitation of the short-latency excitatory postsynaptic potential (EPSP) at short interstimulus intervals (20–200 ms). Trains of stimulation at longer interstimulus intervals (1–30 s) resulted in a depression of EPSP amplitude. The time course of the synaptic depression was compared with that found in EPSPs resulting from paired-pulse stimulation of the dorsal root and found to be comparable. 5. The short-latency MLF EPSP was reversibly blocked by 6-cyano-7-nitroquinoxaline (CNQX), an antagonist of non-N-methyl-D-aspartate glutamate receptors, with a small CNQX-resistant component. Longer-latency components of the MLF EPSP were also blocked by CNQX, and some late components of the PSP were sensitive to strychnine. MLF activation of multiple polysynaptic pathways in the spinal cord is discussed.

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Modulation of somatosensory evoked fields from SI and SII by acute GABA A -agonism and paired-pulse stimulation

NeuroImage ◽

10.1016/j.neuroimage.2007.12.024 ◽

2008 ◽

Vol 40 (2) ◽

pp. 427-434 ◽

Cited By ~ 40

Author(s):

Juha Huttunen ◽

Eero Pekkonen ◽

Reetta Kivisaari ◽

Taina Autti ◽

Seppo Kähkönen

Keyword(s):

Gaba A ◽

Paired Pulse ◽

Somatosensory Evoked Fields ◽

Evoked Fields ◽

Pulse Stimulation

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Facilitatory effect of paired-pulse stimulation by transcranial magnetic stimulation with biphasic wave-form

Medical Engineering & Physics ◽

10.1016/j.medengphy.2016.04.025 ◽

2016 ◽

Vol 38 (8) ◽

pp. 813-817 ◽

Cited By ~ 7

Author(s):

Petro Julkunen ◽

Gustaf Järnefelt ◽

Petri Savolainen ◽

Jarmo Laine ◽

Jari Karhu

Keyword(s):

Transcranial Magnetic Stimulation ◽

Magnetic Stimulation ◽

Facilitatory Effect ◽

Wave Form ◽

Paired Pulse ◽

Pulse Stimulation

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Characterization of the kinetic properties of tetraethylammonium (TEA)-sensitive potassium conductance in a mathematical model of snail neurons

Advanced Studies in Theoretical Physics ◽

10.12988/astp.2019.9417 ◽

2019 ◽

Vol 13 (4) ◽

pp. 189-194

Author(s):

Takaaki Shirahata

Keyword(s):

Mathematical Model ◽

Potassium Conductance ◽

Kinetic Properties ◽

Snail Neurons

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Specific CA3 neurons decode neural information of dentate granule cells evoked by paired-pulse stimulation in co-cultured networks

2017 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) ◽

10.1109/embc.2017.8037643 ◽

2017 ◽

Author(s):

Daniele Poli ◽

Thomas B. DeMarse ◽

Bruce C. Wheeler ◽

Gregory J. Brewer

Keyword(s):

Granule Cells ◽

Paired Pulse ◽

Dentate Granule Cells ◽

Neural Information ◽

Ca3 Neurons ◽

Cultured Networks ◽

Pulse Stimulation

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Augmented aftercontractions in papillary muscles from rats with cardiac hypertrophy

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1979.237.6.h649 ◽

1979 ◽

Vol 237 (6) ◽

pp. H649-H654 ◽

Cited By ~ 2

Author(s):

L. J. Heller

Keyword(s):

General Characteristic ◽

Papillary Muscles ◽

Hypertensive Rats ◽

Spontaneously Hypertensive ◽

Paired Pulse ◽

Time To Peak ◽

High Calcium ◽

Tension Time ◽

Wistar Kyoto ◽

Pulse Stimulation

Paired-pulse stimulation induced larger aftercontractions in papillary muscles from spontaneously hypertensive rats (SHR) than from normotensive Wistar-Kyoto rats (WKY). To determine whether aftercontraction exaggeration is a general characteristic of hypertrophied cardiac muscle, three models were examined: SHRs, deoxycorticosterone acetate (DOCA)-treated rats, and aorta-constricted rats. Responses of pipillary muscles from hypertrophied hearts tested under conditions conducive to aftercontraction generation were compared to pipillary muscles from WKY or sham-treated controls, respectively. Field-stimulated papillary muscles mounted in an oxygenated temperature-controlled physiologic salt solution were exposed to calcium concentrations of 2.5 and 5.0 mM, and temperatures of 27 degrees C and 17 degrees C. Although testing conditions influenced the contractile responses to single stimuli, there was no difference in active tension, time to peak tension, or one-half relaxation time between the three experimental groups and their respective controls. Paired-pulse stimulation induced aftercontractions that were enhanced in high-calcium and low-temperature solutions. Under these conditions, papillary muscles from hypertrophied hearts developed larger aftercontractions than did their respective controls.

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