Molecular structure of allelic variants of microsatellite loci Du281 and Du47 in unisexual and bisexual lizard species of the genus Darevskia

The spiny-tailed lizard, which has a series of taxonomic revisions, is one of the most common lizard species in Turkey. In this study, sequence data derived from three microsatellite loci (Du215, Du281, and Du323), two mitochondrial (16S rRNA and Cyt-b) genes and combined data were used to evaluate the taxonomic status of Darevskia rudis and Darevskia bithynica with new samples from all subspecies populations in Turkey. Our results indicated that the genetic variations of microsatellite loci were not specific to populations within species, and only minor differences separated D. rudis and D. bithynica populations. Furthermore, the markers we used for phylogenetic analyses (NJ, ML, MP, and BI) produced topologically similar trees based on 16S rRNA and Cyt-b while the combined data produced conflicting trees with the separate gene analyses. Finally, the basal relationships among the populations in Turkish populations D. rudis and D. bithynica were not resolved with this dataset, and we found a hard polytomy at the basis of the phylogeny.

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Molecular structure of the allelic variants of (AAT)n microsatellite locus Du47D in the parthenogenetic species Darevskia unisexualis and bisexual parental species D. valentini and D. raddei

Russian Journal of Genetics ◽

10.1134/s1022795410050182 ◽

2010 ◽

Vol 46 (5) ◽

pp. 630-632 ◽

Cited By ~ 2

Author(s):

V. I. Korchagin ◽

O. N. Tokarskaya

Keyword(s):

Molecular Structure ◽

Microsatellite Locus ◽

Parental Species ◽

Allelic Variants ◽

Parthenogenetic Species ◽

And Bisexual

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Analysis of genetic variation in unisexual and bisexual lizard species of the genus Leiolepis from Southeast Asia

Russian Journal of Genetics ◽

10.1134/s1022795406050012 ◽

2006 ◽

Vol 42 (5) ◽

pp. 463-467 ◽

Cited By ~ 7

Author(s):

D. N. Malysheva ◽

I. S. Darevsky ◽

O. N. Tokarskaya ◽

V. G. Petrosyan ◽

I. A. Martirosyan ◽

...

Keyword(s):

Genetic Variation ◽

Southeast Asia ◽

Lizard Species ◽

And Bisexual

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Molecular and genetic characterization of the allelic variants of Du215, Du281, Du323, and Du47G microsatellite loci in parthenogenetic lizard Darevskia armeniaca (Lacertidae)

Russian Journal of Genetics ◽

10.1134/s1022795417040068 ◽

2017 ◽

Vol 53 (4) ◽

pp. 472-482 ◽

Cited By ~ 1

Author(s):

A. E. Girnyk ◽

A. A. Vergun ◽

A. V. Omelchenko ◽

V. G. Petrosyan ◽

V. I. Korchagin ◽

...

Keyword(s):

Microsatellite Loci ◽

Genetic Characterization ◽

Allelic Variants

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Molecular structure and genealogical characterization of the DYS458.2 allelic variants founded in Turkey population samples

Forensic Science International Genetics Supplement Series ◽

10.1016/j.fsigss.2011.08.136 ◽

2011 ◽

Vol 3 (1) ◽

pp. e281-e282 ◽

Cited By ~ 3

Author(s):

A. Serin ◽

H. Canan ◽

B. Alper ◽

D. Kotan

Keyword(s):

Molecular Structure ◽

Allelic Variants

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Genetic Characterization of Six Stocks ofLitopenaeus vannameiUsed in Cuba for Aquaculture by Means of Microsatellite Loci

International Journal of Zoology ◽

10.1155/2012/352165 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7

Author(s):

Anna Pérez-Beloborodova ◽

Adriana Artiles-Valor ◽

Lourdes Pérez-Jar ◽

Damir Hernández-Martínez ◽

Missael Guerra-Aznay ◽

...

Keyword(s):

Litopenaeus Vannamei ◽

Microsatellite Loci ◽

First Generation ◽

Second Generation ◽

Genetic Characterization ◽

Parental Population ◽

Allelic Variants ◽

The Third ◽

Private Alleles

Four microsatellite loci were used to achieve genetic characterization of six stocks fromLitopenaeus vannameiused for aquaculture in Cuba: second generation from first introduction (S2-1), first generation from the second one (S1-2), from the third one (S1-3), and the fourth one (S1-4) and the crossings from two parental population: first generation from the first with first generation from the third (S1-1 × S1-3) and first generation from the second with first generation from the third (S1-2 × S1-3). 66% (16/24) of genetic systems in total loci were in genetic disequilibrium. The four microsatellite loci were polymorphic for all six stocks. Major quantities of allelic variants correspond to locus Pvan 1758, which is at the same time that one where there are private alleles from first generation of the third. All Fst comparisons were significant. This indicates big differences between stocks. The highest values are those in which there is presence of the second introduction. This introduction and its descendants are also more consanguineous.

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Are genetically distinct lizard species able to hybridize? A review

Current Zoology ◽

10.1093/czoolo/61.1.155 ◽

2015 ◽

Vol 61 (1) ◽

pp. 155-180 ◽

Cited By ~ 23

Author(s):

Jitka Jančúchová-Lásková ◽

Eva Landová ◽

Daniel Frynta

Keyword(s):

Reproductive Isolation ◽

Genetic Divergence ◽

Divergence Time ◽

Distinct Species ◽

F1 Hybrids ◽

Lizard Species ◽

And Bisexual ◽

Reproductive Isolation Mechanisms ◽

Cyt B Gene ◽

The Relationship

Abstract Animal species are delimited by reproductive isolation mechanisms (RIMs). Postzygotic RIMs are mainly products of genetic differences and thus their strength increases with elapsed divergence time. The relationship between postzygotic reproductive isolation and genetic divergence, however, differs considerably among major clades of vertebrates. We reviewed the available literature providing empirical evidence of natural and/or experimental hybridization between distinct species of lizards (squamates except snakes). We found that hybridization events are widely distributed among nearly all major lizard clades. The majority of research focuses on parthenogenetic species and/or polyploid hybrids in families Lacertidae, Teiidae and Gekkonidae. Homoploid bisexual hybrids are mainly reported within Lacertidae and Iguania groups. As a proxy of genetic divergence of the hybridizing taxa we adopted nucleotide sequence distance (HKY85) of mitochondrial cyt b gene. The upper limit of genetic divergence was similar with regard to both parthenogenetic and bisexual hybrids. Maximum values of these distances between hybridizing species of lizards approached 18%?21%, which is comparable to or even exceeds the corresponding values reported for other principal clades of vertebrates. In spite of this, F1 hybrids are typically at least partially fertile in lizards and thus genetic in-trogression between highly divergent species is possible. The relationship between the genetic distance and hybrid fertility was not found.

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