On the Effect of Products Made from Recombinant Spidroin Protein on Wound Regeneration in an Experiment

Aim.To perform a comparison of three-dimensional nanostructure of porous biocompatible scaffolds made of fibroinBombix moriand recombinant spidroin rS1/9.Materials and methods.Three-dimensional porous scaffolds were produced by salt leaching technique. The comparison of biological characteristics of the scaffolds shows that adhesion and proliferation of mouse fibroblastsin vitroon these two types of scaffolds do not differ significantly. Comparative experimentsin vivoshow that regeneration of bone tissue of rats is faster with implantation of recombinant spidroin scaffolds. Three-dimensional nanostructure of scaffolds and interconnectivity of nanopores were studied with scanning probe nanotomography (SPNT) to explain higher regenerative activity of spidroin-based scaffolds.Results.Significant differences were detected in the integral density and volume of pores: the integral density of nanopores detected on 2D AFM images is 46 μm–2 and calculated volume porosity is 24% in rS1/9-based scaffolds; in fibroin-based three-dimensional structures density of nanopores and calculated volume porosity were 2.4 μm–2 and 0.5%, respectively. Three-dimensional reconstruction system of nanopores and clusters of interconnected nanopores in rS1/9-based scaffolds showed that volume fraction of pores interconnected in percolation clusters is 35.3% of the total pore volume or 8.4% of the total scaffold volume.Conclusion.Scanning probe nanotomography method allows obtaining unique information about topology of micro – and nanopore systems of artificial biostructures. High regenerative activity of rS1/9-based scaffolds can be explained by higher nanoporosity of the scaffolds.

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A study of biomedical properties of hydrogels based on recombinant spidroin after their sterilization

Polymer Science Series D ◽

10.1134/s199542121602012x ◽

2016 ◽

Vol 9 (2) ◽

pp. 219-222

Author(s):

O. A. Legon’kova ◽

I. P. Savchenkova ◽

M. S. Belova ◽

A. I. Korotaeva ◽

L. I. Davydova ◽

...

Keyword(s):

Recombinant Spidroin

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Characterization of biodegradable cell micro and macro carriers based on recombinant spidroin

Applied Biochemistry and Microbiology ◽

10.1134/s000368381408002x ◽

2014 ◽

Vol 50 (8) ◽

pp. 780-788 ◽

Cited By ~ 3

Author(s):

V. G. Bogush ◽

L. I. Davydova ◽

M. M. Moisenovich ◽

K. V. Sidoruk ◽

A. Yu. Arkhipova ◽

...

Keyword(s):

Recombinant Spidroin

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Tissue Engineered Neural Constructs Composed of Neural Precursor Cells, Recombinant Spidroin and PRP for Neural Tissue Regeneration

Scientific Reports ◽

10.1038/s41598-019-39341-9 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 12

Author(s):

V. P. Baklaushev ◽

V. G. Bogush ◽

V. A. Kalsin ◽

N. N. Sovetnikov ◽

E. M. Samoilova ◽

...

Keyword(s):

Tissue Regeneration ◽

Neural Tissue ◽

Precursor Cells ◽

Neural Precursor Cells ◽

Neural Precursor ◽

Recombinant Spidroin

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Recombinant Spidroin Films Attenuate Individual Markers of Glucose Induced Aging in NIH 3T3 Fibroblasts

Biochemistry (Moscow) ◽

10.1134/s0006297920070093 ◽

2020 ◽

Vol 85 (7) ◽

pp. 808-819

Author(s):

A. M. Moysenovich ◽

M. M. Moisenovich ◽

A. K. Sudina ◽

V. V. Tatarskiy ◽

A. I. Khamidullina ◽

...

Keyword(s):

3T3 Fibroblasts ◽

Nih 3T3 ◽

Recombinant Spidroin

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Functional Analysis of the Engineered Cardiac Tissue Grown on Recombinant Spidroin Fiber Meshes

PLoS ONE ◽

10.1371/journal.pone.0121155 ◽

2015 ◽

Vol 10 (3) ◽

pp. e0121155 ◽

Cited By ~ 10

Author(s):

Alexander Teplenin ◽

Anna Krasheninnikova ◽

Nadezhda Agladze ◽

Konstantin Sidoruk ◽

Olga Agapova ◽

...

Keyword(s):

Functional Analysis ◽

Cardiac Tissue ◽

Recombinant Spidroin

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Progress in the application of spider silk protein in medicine

Journal of Biomaterials Applications ◽

10.1177/08853282211003850 ◽

2021 ◽

pp. 088532822110038

Author(s):

Yi Liu ◽

Wei Huang ◽

Minsi Meng ◽

Minhui Chen ◽

Chengjian Cao

Keyword(s):

Spider Silk ◽

Silk Protein ◽

Large Molecular Weight ◽

Alternative Materials ◽

Spider Silk Protein ◽

Surgical Sutures ◽

Main Protein ◽

Biomaterial Application ◽

Derivatives Of ◽

Recombinant Spidroin

Spider silk protein has attracted much attention on account of its excellent mechanical properties, biodegradability, and biocompatibility. As the main protein component of spider silk, spidroin plays important role in spider spinning under natural circumstances and biomaterial application in medicine as well. Compare to the native spidroin which has a large molecular weight (>300 kDa) with highly repeat glycine and polyalanine regions, the recombinant spidroin was maintained the core amino motifs and much easier to collect. Here, we reviewed the application of recombinant spider silk protein eADF4(C16), major ampullate spidroin (MaSp), minor ampullate spidroin (MiSp), and the derivatives of recombinant spider silk protein in drug delivery system. Moreover, we also reviewed the application of spider silk protein in the field of alternative materials, repairing materials, wound dressing, surgical sutures along with advances in recombinant spider silk protein.

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