scholarly journals Obtaining of transgenic potato plants (Solanum tuberosum L.) that contain antisense sequence of prolindehydrogenase gene

2018 ◽  
Vol 22 ◽  
pp. 299-304
Author(s):  
O. O. Ovcharenko ◽  
V. A. Rudas ◽  
N. L. Shcherbak ◽  
M. V. Kuchuk
Keyword(s):  
Solanum Tuberosum ◽  
Transgenic Plants ◽  
Genetic Transformation ◽  
Solanum Tuberosum L ◽  
Practical Interest ◽  
Transgenic Potato ◽  
Proline Dehydrogenase ◽  
Potato Plants ◽  
Antisense Sequence ◽  
Transgenic Potato Plants

Aim. The aim of our work was to obtain transgenic potato plants of Ukrainian varieties with the expression of a double-stranded RNA suppressor of proline dehydrogenase gene. We propose the decrease of proline degradation level and increase of overall proline concentration in obtained transgenic plants. Methods. The Agrobasterium tumefaciens-mediated method of genetic transformation to obtain transgenic plants of potato was used. Internodes of aseptic potato plants were transformed with a binary vector pBi2E containing an inverted repeats of two copies of proline dehydrogenase gene’s first exon and the gene of neomycin phosphotransferase II (nptII). Results. As a result of experiments kanamycin resistant transgenic potato lines of Deseiree, Belarusian 12 and Slavianka varieties were obtained. The transgenic nature of the obtained plants was confirmed by PCR with primers specific to the first exon of proline dehydrogenase and to nptII genes. Conclusions. The optimized conditions of genetic transformation and used agrobacterial strain allow to obtain the transgenic plants of a model potato variety Désirée, as well as varieties Belorussian 12 and Slovyanka which are of practical interest for cultivation in Ukraine. Keywords: transgenic plants, potatoes (Solanum tuberosum L.), stress resistance, proline.

scholarly journals Design of expression cassettes using the Cry1Ba1 gene for potato (Solanum tuberosum L.) varieties

2019 ◽  
Vol 37 (2) ◽  
pp. 103-111
Author(s):  
Natalyth Erira ◽  
Alejandro Chaparro-Giraldo ◽  
Silvio López-Pazos
Keyword(s):  
Solanum Tuberosum L ◽  
Potato Crop ◽  
Insect Pest ◽  
Transgenic Potato ◽  
Economic Losses ◽  
Potato Plants ◽  
Commercial Use ◽  
Tecia Solanivora ◽  
Transgenic Potato Plants ◽  
Polyadenylation Signals

The most serious insect pest problem in the potato crop in Colombia is the lepidopteran Tecia solanivora that causessignificant economic losses. In this research, we designedexpression cassettes based on the cry1Ba1 gene of Bacillusthuringiensis that could confer resistance to T. solanivora via the variety Pastusa Suprema. We selected the elements of thedesigned expression cassettes through an analysis of scientificliterature and patent databases; the considered factors were theproteolytic activation of the Cry1Ba1 protoxin, modification ofcodonic use, polyadenylation signals, and cryptic splicing sites. We used a tissue-specific patatine promoter to reduce potentialbiosafety risks, because it is expressed only in the tuber. Thefreedom to operate analysis suggests that the commercial use of the designed expression cassettes in transgenic potato plants does not affect the rights of third parties in Colombia.

scholarly journals PVYNTN-CP coat protein gene mediated virus resistance of transgenic potato plants

2009 ◽  
Vol 7 (4) ◽  
pp. 41-50 ◽  
Author(s):  
Zenon Stasevski ◽  
Olga N Ilinskaya
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Virus Resistance ◽  
Protein Gene ◽  
Solanum Tuberosum L ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants ◽  
Transgenic Potatoes ◽  
Virus Strains

PVY<sup style="line-height:1.6em">NTN</sup>-CP <span style="line-height:1.6em">coat protein gene from a necrotic strain of potato virus </span>Y (pvy<sup style="line-height:1.6em">ntn</sup>) <span style="line-height:1.6em">has been transferred into two potato </span>Solanum tuberosum L. <span style="line-height:1.6em">cultivars </span>Mindenes <span style="line-height:1.6em">and </span>Somogyi kifli via Agrobacterium tumefaciens <span style="line-height:1.6em">transformation. Expression of integrated PVY</span><sup style="line-height:1.6em">NTN</sup><span style="line-height:1.6em">-CP gene were confirmed for 33 (89 %) of 37 and 3 (75 %) of 4 kanamycin-resistant regenerants of potato cultivars Mindenes and Somogyi kifli respectively. The level of virus resistance against two virus strains </span>(PVY°, PVY<sup style="line-height:1.6em">NTN</sup>) <span style="line-height:1.6em">of independent lines of transgenic potatoes varied between extreme resistance to susceptibility. The three independent lines of transgenic potatoes proved to be extreme resistant against both PVY strains.</span>

scholarly journals Copper and nickel resistance at ipt-transgenic potato plants

2004 ◽  
Vol 2 (3) ◽  
pp. 25-31
Author(s):  
Elena A Andreeva ◽  
Ludmila A Lutova
Keyword(s):  
Transgenic Plants ◽  
Copper Sulfate ◽  
Transgenic Potato ◽  
Nickel Chloride ◽  
Nickel Resistance ◽  
Potato Plants ◽  
Agrobacterial Transformation ◽  
Enhanced Resistance ◽  
Transgenic Potato Plants

Using agrobacterial transformation collection of /pr-transgenic plants based on cv. Adretta was obtained. Analysis of transgenic plants susceptibility to the exogenous phytohormones auxin (IAA) and cytokinin (kinetin) for 18 from 22 analyzed forms reveals altered reaction to hormones. Among 18 plants with altered reaction to phytohormones for 16 forms enhanced resistance to copper sulfate and/or nickel chloride was observed

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