SAT0370 Synergistic Effect of Adipose Stem Cells and Bone Marrow Stem Cells to Promote Osteogenic Differentiation and Angiogenesis

Bone regeneration is a complex process regulated by several factors that control overlapping biological processes, coordinating interactions among distinct cell populations. There is a great interest in identifying new strategies for inducing osteogenesis in a safe and efficient manner. Concentrated Growth Factor (CGF) is an autologous blood derived product obtained by centrifugation of venous blood following the procedure set on the Silfradent device. In this study the effects of CGF on osteogenic differentiation of human Bone Marrow Stem Cells (hBMSC) in vitro have been investigated; hBMSC were cultured with CGF or osteogenic medium, for 21 days. The osteogenic differentiation was evaluated measuring alkaline phosphatase (ALP) enzyme activity, matrix mineralization by alizarin red staining and through mRNA and protein quantification of osteogenic differentiation markers by Real-time PCR and Western blotting, respectively. The treatment with CGF stimulated ALP activity and promoted matrix mineralization compared to control and seems to be more effective than osteogenic medium. Also, hBMSC lost mesenchymal markers and showed other osteogenic features. Our study showed for the first time that CGF alone is able to induce osteogenic differentiation in hBMSC. The application of CGF on hBMSC osteoinduction might offer new clinical and biotechnological strategies in the tissue regeneration field.

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Osteoblastogenesis Alters Small RNA Profiles in EVs Derived from Bone Marrow Stem Cells (BMSCs) and Adipose Stem Cells (ASCs)

Biomedicines ◽

10.3390/biomedicines8100387 ◽

2020 ◽

Vol 8 (10) ◽

pp. 387

Author(s):

Yan Yan ◽

Clare Chang ◽

Junyi Su ◽

Morten T. Venø ◽

Jørgen Kjems

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Small Rna ◽

Expression Profiles ◽

Osteoblastic Differentiation ◽

Bone Marrow Stem Cells ◽

Adipose Stem Cells ◽

Special Focus ◽

Paracrine Factors ◽

Specific Subset

Multipotent stem cells (MSCs) are used in various therapeutic applications based on their paracrine secretion activity. Here, we set out to identify and characterize the paracrine factors released during osteoblastogenesis, with a special focus on small non-coding RNAs released in extracellular vesicles (EVs). Bone marrow stem cells (BMSCs) and adipose stem cells (ASCs) from healthy human donors were used as representatives of MSCs. We isolated EVs secreted before and after induction of osteoblastic differentiation and found that the EVs contained a specific subset of microRNAs (miRNAs) and tRNA-derived small RNAs (tsRNA) compared to their parental cells. Osteoblastic differentiation had a larger effect on the small RNA profile of BMSC-EVs relative to ASC-EVs. Our data showed that EVs from different MSC origin exhibited distinct expression profiles of small RNA profiles when undergoing osteoblastogenesis, a factor that should be taken into consideration for stem cell therapy.

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Influence of patterned titanium coatings on polarization of macrophage and osteogenic differentiation of bone marrow stem cells

Journal of Biomaterials Applications ◽

10.1177/0885328217746802 ◽

2017 ◽

Vol 32 (7) ◽

pp. 977-986 ◽

Cited By ~ 6

Author(s):

Zequan Zhang ◽

Youtao Xie ◽

Houhua Pan ◽

Liping Huang ◽

Xuebin Zheng

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Osteogenic Differentiation ◽

Bone Marrow Stem Cells ◽

Titanium Coatings

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Comparison of Bone Regeneration in Rabbit Bone Marrow Stem Cells and Adipose Stem Cells

Plastic & Reconstructive Surgery ◽

10.1097/01.prs.0000421820.70229.f2 ◽

2012 ◽

Vol 130 ◽

pp. 104

Author(s):

Daniel Seungyoul Han

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Bone Regeneration ◽

Bone Marrow Stem Cells ◽

Adipose Stem Cells ◽

Rabbit Bone

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Effect of Platelet Releasate on Osteogenic Differentiation of Human Mesenchymal Bone Marrow Stem Cells

Bulletin of Experimental Biology and Medicine ◽

10.1007/s10517-014-2396-1 ◽

2014 ◽

Vol 156 (4) ◽

pp. 560-565 ◽

Cited By ~ 6

Author(s):

S. M. Kosmacheva ◽

N. N. Danilkovich ◽

A. V. Shchepen’ ◽

S. I. Ignatenko ◽

M. P. Potapnev

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Osteogenic Differentiation ◽

Bone Marrow Stem Cells ◽

Platelet Releasate

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LMCD1 promotes osteogenic differentiation of human bone marrow stem cells by regulating BMP signaling

Cell Death and Disease ◽

10.1038/s41419-019-1876-7 ◽

2019 ◽

Vol 10 (9) ◽

Cited By ~ 1

Author(s):

Bin Zhu ◽

Feng Xue ◽

Changqing Zhang ◽

Guangyi Li

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Osteogenic Differentiation ◽

Adipogenic Differentiation ◽

Bone Marrow Stem Cells ◽

Bmp Signaling ◽

Human Bone ◽

Human Bone Marrow ◽

Bone Homeostasis

Abstract Human bone marrow stem cells (BMSCs) are heterogeneous progenitor cells with two defining features, self-renew and multi-lineage differentiation. As one of the differentiation directions, osteogenesis is vital for bone homeostasis. A growing body of evidences show that ubiquitin-dependent protein degradation plays an essential role in the osteogenic differentiation of BMSCs. In this study, we found that LMCD1 was upregulated during osteogenic differentiation process of BMSCs by analyzing GSE80614. In vitro and in vivo functional studies confirmed that LMCD1 was critical to the osteogenic commitment of BMSCs. Compared to those of the controls, downregulation of LMCD1 significantly restrained osteogenic differentiation and enhanced adipogenic differentiation, while upregulation of LMCD1 increased the osteogenic differentiation and suppressed adipogenic differentiation. Mechanically, we found that LMCD1 could protect RUNX2 and Smad1 protein from Smurf1-induced ubiquitination degradation thereby regulating BMP signaling. In conclusion, our findings suggest that LMCD1 is a novel regulator of osteogenic differentiation and may be a potential therapeutic target for bone metabolism related diseases.

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Identification of suitable culture condition for expansion and osteogenic differentiation of human bone marrow stem cells

Human Cell ◽

10.1007/s13577-012-0045-4 ◽

2012 ◽

Vol 25 (3) ◽

pp. 69-77 ◽

Cited By ~ 4

Author(s):

Shiplu Roy Chowdhury ◽

Min Hwei Ng ◽

Norazril Shamsul Abu Hassan ◽

Bin Saim Aminuddin ◽

Bt Hj Idrus Ruszymah

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Osteogenic Differentiation ◽

Culture Condition ◽

Bone Marrow Stem Cells ◽

Human Bone ◽

Human Bone Marrow

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Synergistic Effect of Platelet-rich Fibrin Releasate and Bone Marrow Stem Cells in Preventing Bone Loss in Ovariectomized Mouse

10.21203/rs.3.rs-27529/v1 ◽

2020 ◽

Author(s):

chin chean wong ◽

Jeng-Hao Liao ◽

Shi-Yuan Sheu ◽

Po-Yu Lin ◽

Chih-Hwa Chen ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Synergistic Effect ◽

Untreated Control ◽

Bone Marrow Stem Cells ◽

Therapeutic Effects ◽

Mineral Density ◽

Platelet Rich Fibrin ◽

Injection Group ◽

Bone Disorder

Abstract Background:Osteoporosis is a metabolic bone disorder characterized by deterioration in the quantity and quality of bone tissue, with a consequent increase susceptibility to fracture.Methods: In this study, we sought to determine the efficacy of platelet-rich fibrin releasate (PRFr) in augmenting the therapeutic effects of stem cell-based therapy in treating osteoporotic bone disorder. An osteoporosis mouse model was established through bilateral ovariectomy on 12 week-old female ICR (Institute of Cancer Research) mice. Eight weeks postoperatively, the ovariectomized(OVX) mice were left untreated (control) or injected with PRFr, bone marrow stem cells (BMSCs), or the combination of BMSCs and PRFr. Two different injection (single versus quadruple)dosages were testedto investigate the accumulative effects of BMSCS and PRFr on bone quality. Eightweeksafter injection, the changes in tibial microstructural profiles included the percentage of bone volume versus total tissue volume (BV/TV, %), bone mineral density (BMD, g/cm3), trabecular number (Tb.N, number/mm), and trabecular separation (Tb.Sp, mm) and bony histology were analyzed. Results: Postmenopausal osteoporosismodel was successfully established in OVX mice, evidenced by reduced BMD, decreased BV/TV, lower Tb.N but increased Tb.Sp..Eight weeks after injection, there was no significant changes to BMD and bone trabeculae could be detected in mice that received low-dose regimen. In contrast, in mice which received 4 doses of combined PRFr and BMSCs, the BMD, BV/TV, and TB.N increased, and the TB.Sp decreased significantly compared to untreated OVX mice. Moreover, the histological analysis showed the trabecular spacingbecome narrower in OVX-mice treated with high-dose injection of BMSCs and combined PRFr and BMSCs than untreated control.Conclusion: The systemic administration of combined BMSCs and PRFr protected against OVX-induced bone mass loss in mice. Moreover, the improvement of bony profile scores in quadruple injection group is better than the single-injection group, probably through the increase in effect size of cells and growth factors. Our data also revealed the combination therapy of BMSCs and PRFr has a synergistic effect in enhancing osteogenesis, which may provide insight for the development of a novel therapeutic strategy in osteoporosis treatment.

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