Concentrated Growth Factors (CGF) Induce Osteogenic Differentiation in Human Bone Marrow Stem Cells

Bone regeneration is a complex process regulated by several factors that control overlapping biological processes, coordinating interactions among distinct cell populations. There is a great interest in identifying new strategies for inducing osteogenesis in a safe and efficient manner. Concentrated Growth Factor (CGF) is an autologous blood derived product obtained by centrifugation of venous blood following the procedure set on the Silfradent device. In this study the effects of CGF on osteogenic differentiation of human Bone Marrow Stem Cells (hBMSC) in vitro have been investigated; hBMSC were cultured with CGF or osteogenic medium, for 21 days. The osteogenic differentiation was evaluated measuring alkaline phosphatase (ALP) enzyme activity, matrix mineralization by alizarin red staining and through mRNA and protein quantification of osteogenic differentiation markers by Real-time PCR and Western blotting, respectively. The treatment with CGF stimulated ALP activity and promoted matrix mineralization compared to control and seems to be more effective than osteogenic medium. Also, hBMSC lost mesenchymal markers and showed other osteogenic features. Our study showed for the first time that CGF alone is able to induce osteogenic differentiation in hBMSC. The application of CGF on hBMSC osteoinduction might offer new clinical and biotechnological strategies in the tissue regeneration field.

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MicroRNA-320a Regulates the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by Targeting HOXA10

Cellular Physiology and Biochemistry ◽

10.1159/000438607 ◽

2016 ◽

Vol 38 (1) ◽

pp. 40-48 ◽

Cited By ~ 30

Author(s):

Jianhou Huang ◽

Yake Meng ◽

Yan Liu ◽

Yu Chen ◽

Haisong Yang ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Osteogenic Differentiation ◽

Ectopic Expression ◽

In Silico Analysis ◽

Human Bone ◽

Human Bone Marrow ◽

Matrix Mineralization ◽

Morphogenetic Protein

Background/Aims: Human bone marrow-derived mesenchymal stem cells (hMSCs) are a promising cell source for bone engineering owing to their high potential to differentiate into osteoblasts. The bone morphogenetic protein-inducible gene homeobox a10 (HOXA10) is a critical regulator of osteogenesis. The objective of the present study was to identify microR-NAs (miRNAs) targeting HOXA10 and examine the effects on the osteogenic differentiation of hMSCs. Methods: Based on in silico analysis, HOXA10-targeting miRNAs were selected and their regulatory roles in osteoblast differentiation were investigated. Results: Six HOXA10-targeting miRNAs were identifIed by computational analysis, of which miR-320a was selected for further analysis because it was downregulated during osteogenic induction. Overexpression of miR-320a downregulated HOXA10 and significantly inhibited osteogenesis in hMSCs, as determined by the downregulation of the osteogenic markers Runx2, ALP, and OC and the inhibition of ALP activity and matrix mineralization, whereas miR-320a inhibition had the opposite effects. Furthermore, ectopic expression of HOXA10 (not including 3′-UTR) rescued the effects of miR-320a on osteogenic differentiation. Conclusion: These results suggest that miR-320a acts as a critical regulator of osteogenic differentiation of hMSCs by repressing its target HOXA10.

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LMCD1 promotes osteogenic differentiation of human bone marrow stem cells by regulating BMP signaling

Cell Death and Disease ◽

10.1038/s41419-019-1876-7 ◽

2019 ◽

Vol 10 (9) ◽

Cited By ~ 1

Author(s):

Bin Zhu ◽

Feng Xue ◽

Changqing Zhang ◽

Guangyi Li

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Osteogenic Differentiation ◽

Adipogenic Differentiation ◽

Bone Marrow Stem Cells ◽

Bmp Signaling ◽

Human Bone ◽

Human Bone Marrow ◽

Bone Homeostasis

Abstract Human bone marrow stem cells (BMSCs) are heterogeneous progenitor cells with two defining features, self-renew and multi-lineage differentiation. As one of the differentiation directions, osteogenesis is vital for bone homeostasis. A growing body of evidences show that ubiquitin-dependent protein degradation plays an essential role in the osteogenic differentiation of BMSCs. In this study, we found that LMCD1 was upregulated during osteogenic differentiation process of BMSCs by analyzing GSE80614. In vitro and in vivo functional studies confirmed that LMCD1 was critical to the osteogenic commitment of BMSCs. Compared to those of the controls, downregulation of LMCD1 significantly restrained osteogenic differentiation and enhanced adipogenic differentiation, while upregulation of LMCD1 increased the osteogenic differentiation and suppressed adipogenic differentiation. Mechanically, we found that LMCD1 could protect RUNX2 and Smad1 protein from Smurf1-induced ubiquitination degradation thereby regulating BMP signaling. In conclusion, our findings suggest that LMCD1 is a novel regulator of osteogenic differentiation and may be a potential therapeutic target for bone metabolism related diseases.

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Identification of suitable culture condition for expansion and osteogenic differentiation of human bone marrow stem cells

Human Cell ◽

10.1007/s13577-012-0045-4 ◽

2012 ◽

Vol 25 (3) ◽

pp. 69-77 ◽

Cited By ~ 4

Author(s):

Shiplu Roy Chowdhury ◽

Min Hwei Ng ◽

Norazril Shamsul Abu Hassan ◽

Bin Saim Aminuddin ◽

Bt Hj Idrus Ruszymah

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Osteogenic Differentiation ◽

Culture Condition ◽

Bone Marrow Stem Cells ◽

Human Bone ◽

Human Bone Marrow

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3D and Porous RGDC‐Functionalized Polyester‐Based Scaffolds as a Niche to Induce Osteogenic Differentiation of Human Bone Marrow Stem Cells

Macromolecular Bioscience ◽

10.1002/mabi.201900049 ◽

2019 ◽

Vol 19 (6) ◽

pp. 1900049 ◽

Cited By ~ 5

Author(s):

Mohammed A. Yassin ◽

Tiziana Fuoco ◽

Samih Mohamed‐Ahmed ◽

Kamal Mustafa ◽

Anna Finne‐Wistrand

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Osteogenic Differentiation ◽

Bone Marrow Stem Cells ◽

Human Bone ◽

Human Bone Marrow

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The antimicrobial peptide KR-12 promotes the osteogenic differentiation of human bone marrow stem cells by stimulating BMP/SMAD signaling

RSC Advances ◽

10.1039/c8ra00750k ◽

2018 ◽

Vol 8 (28) ◽

pp. 15547-15557 ◽

Cited By ~ 6

Author(s):

Hui Li ◽

Shutao Zhang ◽

Bin'en Nie ◽

Zhe Du ◽

Teng Long ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Antimicrobial Peptide ◽

Osteogenic Differentiation ◽

Bone Marrow Stem Cells ◽

Human Bone ◽

Human Bone Marrow ◽

Multiple Infections ◽

Smad Signaling

KR-12 is the smallest fragment of human antimicrobial peptide cathelicidin (LL-37), and could play key roles in the treatment of multiple infections, including osteomyelitis.

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Micro/Nano Structural Tantalum Coating for Enhanced Osteogenic Differentiation of Human Bone Marrow Stem Cells

Materials ◽

10.3390/ma11040546 ◽

2018 ◽

Vol 11 (4) ◽

pp. 546 ◽

Cited By ~ 5

Author(s):

Ding Ding ◽

Youtao Xie ◽

Kai Li ◽

Liping Huang ◽

Xuebin Zheng

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Osteogenic Differentiation ◽

Bone Marrow Stem Cells ◽

Human Bone ◽

Human Bone Marrow

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Parathyroid hormone promotes the osteogenesis of lipopolysaccharide-induced human bone marrow mesenchymal stem cells through the JNK MAPK pathway

Bioscience Reports ◽

10.1042/bsr20210420 ◽

2021 ◽

Author(s):

Ziyue Qin ◽

Shu Hua ◽

Huifen Chen ◽

Zhuo Wang ◽

Haoran Wang ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Parathyroid Hormone ◽

Osteogenic Differentiation ◽

Human Bone ◽

Human Bone Marrow ◽

Cell Counting ◽

Alizarin Red ◽

Marrow Mesenchymal Stem Cells

Periodontitis is a series of inflammatory processes caused by bacterial infection. Parathyroid hormone (PTH) plays a critical role in bone remodeling. This study aimed to investigate the influences of PTH on human bone marrow mesenchymal stem cells (HBMSCs) pretreated with lipopolysaccharide (LPS). The proliferative ability was measured using cell counting kit-8 and flow cytometry. The optimal concentrations of PTH and LPS were determined using alkaline phosphatase (ALP) activity assay, ALP staining, and Alizarin red staining. Osteogenic differentiation was further assessed by quantitative reverse transcription–polymerase chain reaction, Western blot analysis, and immunofluorescence staining. PTH had no effects on the proliferation of HBMSCs. Also, 100 ng/mL LPS significantly inhibited HBMSC osteogenesis, while 10−9 mol/L PTH was considered as the optimal concentration to reverse the adverse effects. Mechanistically, c-Jun N-terminal kinase (JNK) phosphorylation was activated by PTH in LPS-induced HBMSCs. SP600125, a selective inhibitor targeting JNK mitogen-activated protein kinase (MAPK) signaling, weakened the effects of PTH. Taken together, the findings revealed the role and mechanism of PTH and JNK pathway in promoting the osteogenic differentiation of LPS-induced HBMSCs, which offered an alternative for treating periodontal diseases.

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CRYAB promotes osteogenic differentiation of human bone marrow stem cells via stabilizing β‐catenin and promoting the Wnt signalling

Cell Proliferation ◽

10.1111/cpr.12709 ◽

2019 ◽

Vol 53 (1) ◽

Cited By ~ 1

Author(s):

Bin Zhu ◽

Feng Xue ◽

Guangyi Li ◽

Changqing Zhang

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Osteogenic Differentiation ◽

Bone Marrow Stem Cells ◽

Wnt Signalling ◽

Human Bone ◽

Human Bone Marrow

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Hyaluronic acid/poly-l-lysine bilayered silica nanoparticles enhance the osteogenic differentiation of human mesenchymal stem cells

Journal of Materials Chemistry B ◽

10.1039/c4tb01071j ◽

2014 ◽

Vol 2 (40) ◽

pp. 6939-6946 ◽

Cited By ~ 33

Author(s):

Sara Amorim ◽

Albino Martins ◽

Nuno M. Neves ◽

Rui L. Reis ◽

Ricardo A. Pires

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Hyaluronic Acid ◽

Osteogenic Differentiation ◽

Silica Nanoparticles ◽

Human Mesenchymal Stem Cells ◽

Bone Marrow Stem Cells ◽

Human Bone ◽

Human Bone Marrow

The coating of silica nanoparticles with a bilayer of poly-l-lysine and hyaluronic acid enhances the osteogenic differentiation of human bone marrow stem cells at low nanoparticle concentrations (25 μg mL−1 and 12.5 μg mL−1).

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