Variation in circulating immune complex levels with diet, exercise, and sleep: a comparison between normal controls and patients with systemic lupus erythematosus.

In the MRL/l mouse, gp70 apparently plays a role as an autoantigen in the development of SLE. However, while gp70 may be an important pathogenetic element, it is not essential to MRL SLE, since elimination of most of the serum gp70 and virtually all of the immune complex gp70 from MRL/l-low gp70 congenic lines had no observable effect on the course or nature of the disease. Thus, while gp70 in the MRL/l mouse appears to be a convenient autoantigenic target when present in significant levels, in its absence the host appears capable of directing its aberrant immunologic responsiveness elsewhere with undiminished pathogenicity.

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Structural polymorphisms of complement receptor 1 (CR1) in systemic lupus erythematosus (SLE) patients and normal controls of three ethnic groups

Clinical & Experimental Immunology ◽

10.1046/j.1365-2249.1996.d01-748.x ◽

1996 ◽

Vol 105 (2) ◽

pp. 302-305 ◽

Cited By ~ 29

Author(s):

J. M. MOULDS ◽

J. D. REVEILLE ◽

F. C. ARNETT

Keyword(s):

Systemic Lupus Erythematosus ◽

Ethnic Groups ◽

Lupus Erythematosus ◽

Complement Receptor ◽

Systemic Lupus ◽

Complement Receptor 1 ◽

Normal Controls

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Gene Expression Profile Reveals Abnormalities of Multiple Signaling Pathways in Mesenchymal Stem Cell Derived from Patients with Systemic Lupus Erythematosus

Clinical and Developmental Immunology ◽

10.1155/2012/826182 ◽

2012 ◽

Vol 2012 ◽

pp. 1-12 ◽

Cited By ~ 32

Author(s):

Yu Tang ◽

Xiaolei Ma ◽

Huayong Zhang ◽

Zhifeng Gu ◽

Yayi Hou ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

Signaling Pathway ◽

Lupus Erythematosus ◽

Protein Level ◽

Mapk Signaling ◽

Signal Pathways ◽

Downregulated Gene ◽

Systemic Lupus ◽

Multiple Signaling ◽

Normal Controls

We aimed to compare bone-marrow-derived mesenchymal stem cells (BMMSCs) between systemic lupus erythematosus (SLE) and normal controls by means of cDNA microarray, immunohistochemistry, immunofluorescence, and immunoblotting. Our results showed there were a total of 1, 905 genes which were differentially expressed by BMMSCs derived from SLE patients, of which, 652 genes were upregulated and 1, 253 were downregulated. Gene ontology (GO) analysis showed that the majority of these genes were related to cell cycle and protein binding. Pathway analysis exhibited that differentially regulated signal pathways involved actin cytoskeleton, focal adhesion, tight junction, and TGF-βpathway. The high protein level of BMP-5 and low expression of Id-1 indicated that there might be dysregulation in BMP/TGF-βsignaling pathway. The expression of Id-1 in SLE BMMSCs was reversely correlated with serum TNF-αlevels. The protein level of cyclin E decreased in the cell cycling regulation pathway. Moreover, the MAPK signaling pathway was activated in BMMSCs from SLE patients via phosphorylation of ERK1/2 and SAPK/JNK. The actin distribution pattern of BMMSCs from SLE patients was also found disordered. Our results suggested that there were distinguished differences of BMMSCs between SLE patients and normal controls.

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Increased levels of anti-dsDNA antibodies in immune complexes before treatment with belimumab associate with clinical response in patients with systemic lupus erythematosus

Arthritis Research & Therapy ◽

10.1186/s13075-019-2056-y ◽

2019 ◽

Vol 21 (1) ◽

Cited By ~ 3

Author(s):

Azita Sohrabian ◽

Ioannis Parodis ◽

Nellie Carlströmer-Berthén ◽

Martina Frodlund ◽

Andreas Jönsen ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

Disease Activity ◽

Immune Complex ◽

Lupus Erythematosus ◽

Immune Complexes ◽

Activity Index ◽

Disease Activity Index ◽

Serum Levels ◽

Systemic Lupus ◽

Double Stranded Dna

Abstract Introduction Immune complexes are of importance in systemic lupus erythematosus pathogenesis, and autoantibodies are believed to participate in immune complex formation. Quantification of autoantibody levels in circulating IC might be of prognostic value. Methods A C1q-binding-eluting technique was applied to purify immune complexes from 55 belimumab-treated systemic lupus erythematosus patients during a 24-month follow-up. Autoantibodies in serum and in solubilized immune complexes were quantified using addressable laser bead immunoassay. We investigated whether levels of autoantibodies in immune complexes associate with disease activity and response to belimumab treatment. Results High baseline anti-double-stranded DNA and anti-histone levels in immune complexes associated with attainment of zero scores in clinical systemic lupus erythematosus disease activity index 2000 during the 24-month follow-up (p = 0.003 and p = 0.048, respectively). Low complement levels associated with high serum anti-double-stranded DNA and anti-ribosomal P levels (p = 0.003 and p = 0.008, respectively) and high anti-double-stranded DNA (p = 0.002) but not anti-ribosomal P levels in immune complexes. Anti-SSA/SSB serum levels were lower in patients attaining lupus low disease activity state at month 6; these associations were stronger for corresponding immune complex levels. Serum levels of most autoantibodies had declined at month 3, whereas autoantibody levels in immune complexes, except for anti-double-stranded DNA, showed a more gradual decline over 1–2 years. Serum anti-double-stranded DNA levels decreased in all patients irrespective of systemic lupus erythematosus disease activity index 2000=0 attainment, whereas immune complex levels decreased only in achievers. Conclusion Immune complex levels of autoantibodies against double-stranded DNA and the SSA/SSB complex show more specific associations with treatment outcome compared with serum levels in belimumab-treated systemic lupus erythematosus patients. Characterization of autoantibody content in circulating immune complexes could prove useful in treatment evaluation in systemic lupus erythematosus and other immune complex-associated diseases.

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