scholarly journals Rapid microbiological assay of serum vitamin B12 by electronic counter

1966 ◽  
Vol 19 (1) ◽  
pp. 46-50 ◽  
Author(s):  
J. Stuart ◽  
S. A. Sklaroff
1987 ◽  
Vol 33 (1) ◽  
pp. 52-54 ◽  
Author(s):  
B P Kelleher ◽  
K G Walshe ◽  
J M Scott ◽  
S D O'Broin

Abstract In this simplified microbiological assay for serum vitamin B12, Lactobacillus leichmanii (NCIB 8117) adapted to tolerate high concentrations (500 mg/L) of the polymyxin antibiotic colistin sulfate is used. Results were similar in parallel experiments in which we used both the parent strain of L. leichmanii (NCIB 8117), and the new adapted strain. Evaluation of assay performance showed excellent analytical recovery of added cyanocobalamin (97%, SD 3%) and good interassay and intra-assay precision (CV less than 5%). This modified assay system obviates the need to sterilize culture medium and glassware. Consequently, assay manipulations may be carried out openly, without aseptic precautions. Moreover, this adapted organism would be suitable for use in an automated microbiological assay system.


1951 ◽  
Vol 193 (1) ◽  
pp. 299-305
Author(s):  
HaroldE. Scheid ◽  
B.S. Schweigert

Author(s):  
C. T. Jones ◽  
J. A. Williams ◽  
E. V. Cox ◽  
W. T. Cooke

2001 ◽  
Vol 23 (3) ◽  
pp. 165-169 ◽  
Author(s):  
Tay S. Kennedy ◽  
Ellen B. Fung ◽  
Deborah A. Kawchak ◽  
Babette S. Zemel ◽  
Kwaku Ohene-Frempong ◽  
...  

2013 ◽  
Vol 36 (1) ◽  
pp. 92-97 ◽  
Author(s):  
A. F. Remacha ◽  
E. Zapico ◽  
M. P. Sarda ◽  
E. Rojas ◽  
M. Simó ◽  
...  

Author(s):  
J.A. Campbell ◽  
J.M. McLaughlan ◽  
Jean A. Clark ◽  
C.W. Dunnett

1981 ◽  
Vol 34 (7) ◽  
pp. 1356-1361 ◽  
Author(s):  
S Ekvall ◽  
I W Chen ◽  
R Bozian

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