Microbiological assay for vitamin B12 with use of a colistin-sulfate-resistant organism.

1987 ◽  
Vol 33 (1) ◽  
pp. 52-54 ◽  
Author(s):  
B P Kelleher ◽  
K G Walshe ◽  
J M Scott ◽  
S D O'Broin
Keyword(s):  
Vitamin B12 ◽  
Serum Vitamin ◽  
Assay System ◽  
Microbiological Assay ◽  
Resistant Organism ◽  
Colistin Sulfate ◽  
Assay Performance ◽  
Analytical Recovery ◽  
High Concentrations ◽  
Assay Precision

Abstract In this simplified microbiological assay for serum vitamin B12, Lactobacillus leichmanii (NCIB 8117) adapted to tolerate high concentrations (500 mg/L) of the polymyxin antibiotic colistin sulfate is used. Results were similar in parallel experiments in which we used both the parent strain of L. leichmanii (NCIB 8117), and the new adapted strain. Evaluation of assay performance showed excellent analytical recovery of added cyanocobalamin (97%, SD 3%) and good interassay and intra-assay precision (CV less than 5%). This modified assay system obviates the need to sterilize culture medium and glassware. Consequently, assay manipulations may be carried out openly, without aseptic precautions. Moreover, this adapted organism would be suitable for use in an automated microbiological assay system.

scholarly journals Rapid microbiological assay of serum vitamin B12 by electronic counter

1966 ◽  
Vol 19 (1) ◽  
pp. 46-50 ◽  
Author(s):  
J. Stuart ◽  
S. A. Sklaroff
Keyword(s):  
Vitamin B12 ◽  
Serum Vitamin ◽  
Microbiological Assay

Evaluation of a new valproic acid enzyme immunoassay and comparison with a capillary gas-chromatographic method.

1981 ◽  
Vol 27 (1) ◽  
pp. 169-172 ◽  
Author(s):  
S L Braun ◽  
A Tausch ◽  
W Vogt ◽  
K Jacob ◽  
M Knedel
Keyword(s):  
Valproic Acid ◽  
Enzyme Immunoassay ◽  
Chromatographic Method ◽  
Comparison Method ◽  
Manual Method ◽  
Analytical Recovery ◽  
Significant Difference ◽  
High Concentrations ◽  
Assay Precision ◽  
Gas Chromatographic Method

Abstract A new homogeneous immunoassay (EMIT) for valproic acid was evaluated. Besides testing the manual version of this enzyme immunoassay, we also developed two mechanized procedures for centrifugal analyzers (the CentrifiChem and the COBAS system), which take less time and are more precise than the manual method. Within-assay precision (CV) was 4.5% with the manual technique and 2% with the analyzers. Between-assay precision (CV) ranged from 4 to 13% for all three techniques. Accuracy of th manual method was checked by dilution and analytical recovery experiments. Our comparison of the EMIT results with those obtained by a comparison method (capillary gas chromatography) showed no significant difference. No interference from hemolysis, hyperbilirubinemia, or aliphatic amino acids was observed. At high concentrations of bile acids and with lipemic sera the analytical recovery rates decreased slightly, to 87% and 92%, respectively.

scholarly journals LIBERATION AND MICROBIOLOGICAL ASSAY OF VITAMIN B12 IN ANIMAL TISSUES

1951 ◽  
Vol 193 (1) ◽  
pp. 299-305
Author(s):  
HaroldE. Scheid ◽  
B.S. Schweigert
Keyword(s):  
Vitamin B12 ◽  
Microbiological Assay ◽  
Animal Tissues

The Incidence and Aetiology of Low Serum Vitamin B12 After Partial Gastrectomy

2015 ◽  
pp. 512-516
Author(s):  
C. T. Jones ◽  
J. A. Williams ◽  
E. V. Cox ◽  
W. T. Cooke
Keyword(s):  
Vitamin B12 ◽  
Serum Vitamin ◽  
Partial Gastrectomy ◽  
Low Serum

Red Blood Cell Folate and Serum Vitamin B12 Status in Children With Sickle Cell Disease

2001 ◽  
Vol 23 (3) ◽  
pp. 165-169 ◽  
Author(s):  
Tay S. Kennedy ◽  
Ellen B. Fung ◽  
Deborah A. Kawchak ◽  
Babette S. Zemel ◽  
Kwaku Ohene-Frempong ◽  
...  
Keyword(s):  
Sickle Cell Disease ◽  
Blood Cell ◽  
Vitamin B12 ◽  
Sickle Cell ◽  
Red Blood Cell ◽  
Serum Vitamin ◽  
Cell Disease

Immune complexes and persistent high levels of serum vitamin B12

2013 ◽  
Vol 36 (1) ◽  
pp. 92-97 ◽  
Author(s):  
A. F. Remacha ◽  
E. Zapico ◽  
M. P. Sarda ◽  
E. Rojas ◽  
M. Simó ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Immune Complexes ◽  
Serum Vitamin

The Six-Point Design in the U. S. P. Microbiological Assay of Vitamin B12

1953 ◽  
Vol 42 (5) ◽  
pp. 276-283 ◽  
Author(s):  
J.A. Campbell ◽  
J.M. McLaughlan ◽  
Jean A. Clark ◽  
C.W. Dunnett
Keyword(s):  
Vitamin B12 ◽  
Microbiological Assay
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