Clinical evaluation of NucliSENS magnetic extraction and NucliSENS analytical specific reagents for the real-time detection of respiratory syncytial virus (RSV) in paediatric respiratory specimens

2009 ◽  
Vol 62 (11) ◽  
pp. 998-1002 ◽  
Author(s):  
R Manji ◽  
M Lotlikar ◽  
F Zhang ◽  
C C Ginocchio
Keyword(s):  
Respiratory Syncytial Virus ◽  
Real Time ◽  
Clinical Evaluation ◽  
The Real ◽  
Real Time Detection ◽  
Syncytial Virus ◽  
Magnetic Extraction ◽  
Respiratory Specimens

scholarly journals Evaluation of reverse transcription-PCR protocols based on the fusion gene for diagnosis of bovine respiratory syncytial virus infections

2013 ◽  
Vol 29 (1) ◽  
pp. 53-64
Author(s):  
A. Selim ◽  
W. Gaede
Keyword(s):  
Respiratory Syncytial Virus ◽  
Real Time ◽  
Internal Control ◽  
Fusion Gene ◽  
Feedlot Cattle ◽  
Bovine Respiratory Syncytial Virus ◽  
Virus Infections ◽  
Rt Pcr ◽  
The Real ◽  
Syncytial Virus

Bovine respiratory syncytial virus (BRSV) is a pneumovirus in the family paramyxoviridae, is an important cause of acute respiratory disease in postweaning calves and feedlot cattle. The real-time reverse transcriptase PCR protocols were developed to detect BRSV infection in infected animals. The sensitivity of RT-PCR protocols based on fusion gene were evaluated using different Mastermixes such as Qiagen One Step RT-PCR (Qiagen) for conventional RT-PCR, Superscrip probe (Invitrogen) and QuentiTec probe (Qiagen) for realtime RT-PCR with and without internal control. The detection limit of different RT-PCR protocols using serial dilutions from BRSV plasmid and based on different probes was 10 RNA copies/ml. Furthermore, the specificity of real-time RT-PCR was evaluated using different bacterial and viral strains which can be isolated from respiratory infected animals. In another side, the real-time RT-PCR in combination with ?-actin and conventional RT-PCR showed detectable Ctvalues only with BRSV strain.

scholarly journals A hybrid upconversion nanoprobe for ratiometric detection of aliphatic biogenic amines in aqueous medium

2021 ◽  
Author(s):  
Shilpi Jaiswal ◽  
Subhankar Kundu ◽  
Sujoy Bandyopadhyay ◽  
Abhijit Patra
Keyword(s):  
Aqueous Medium ◽  
Biogenic Amines ◽  
Real Time ◽  
Inorganic Hybrid ◽  
The Real ◽  
Ratiometric Detection ◽  
Real Time Detection

An organic–inorganic hybrid upconversion nanoprobe was developed for the real-time detection of aliphatic biogenic amines in an aqueous medium, adulterated milk, and rotten fish.

scholarly journals Biosensors for the Multiplex Detection of Inflammatory Disease Biomarkers

Biosensors ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 11
Author(s):  
Ali Mobasheri
Keyword(s):  
Real Time ◽  
Inflammatory Disease ◽  
Multiplex Detection ◽  
The Real ◽  
Disease Biomarkers ◽  
Real Time Detection ◽  
Biochemical Substance ◽  
Analytical Device

A biosensor is an analytical device used for the real-time detection and measurement of a chemical or biochemical substance [...]

Sign in / Sign up

Export Citation Format

Share Document