scholarly journals Sensitive system for visualising biotinylated DNA probes hybridised in situ: rapid sex determination of intact cells.

1985 ◽  
Vol 38 (10) ◽  
pp. 1085-1092 ◽  
Author(s):  
J Burns ◽  
V T Chan ◽  
J A Jonasson ◽  
K A Fleming ◽  
S Taylor ◽  
...  
Keyword(s):  
Sex Determination ◽  
Dna Probes ◽  
Intact Cells

Sex determination of bovine embryos using male-specific DNA probes

1988 ◽  
Vol 29 (1) ◽  
pp. 242 ◽  
Author(s):  
S.B. Ellis ◽  
K.R. Bondioli ◽  
M.E. Williams ◽  
J.H. Pryor ◽  
M.M. Harpold
Keyword(s):  
Sex Determination ◽  
Dna Probes ◽  
Bovine Embryos ◽  
Male Specific

Sex Determination of Bovine Embryo Biopsies Using in situ Hybridization

1991 ◽  
Vol 26 (2) ◽  
pp. 66-69 ◽  
Author(s):  
P.D. Thomsen ◽  
M. Schwerin ◽  
P.H. Poulsen ◽  
B. Avery
Keyword(s):  
In Situ Hybridization ◽  
Sex Determination ◽  
Bovine Embryo

scholarly journals A novel in situ hybridization signal amplification method based on the deposition of biotinylated tyramine.

1995 ◽  
Vol 43 (4) ◽  
pp. 347-352 ◽  
Author(s):  
H M Kerstens ◽  
P J Poddighe ◽  
A G Hanselaar
Keyword(s):  
In Situ Hybridization ◽  
Signal Amplification ◽  
Dna Probes ◽  
Bright Field ◽  
Archival Material ◽  
Amplification Method ◽  
Highly Sensitive ◽  
Catalyzed Reporter Deposition

For amplification of in situ hybridization (ISH) signals, we describe a method using catalyzed reporter deposition (CARD). This amplification method is based on the deposition of biotinylated tyramine (BT) at the location of the DNA probe. The BT precipitate can then visualized with fluorochrome- or enzyme-labeled avidin. Both for bright-field ISH (BRISH) and for fluorescence ISH (FISH), the detection limit was highly increased. This method is especially suitable for visualization of very weak ISH signals, such as those obtained by ISH using locus-specific DNA probes. Furthermore, CARD amplification of ISH signals (CARD-ISH) is highly sensitive, rapid, flexible, and easy to implement. Successful application of CARD-ISH with locus-specific DNA probes on histological and cytological samples may improve the determination of structural chromosomal aberrations in archival material.

In situ determination of [Ca2+]i threshold for translocation of the alpha-protein kinase C isoform

1994 ◽  
Vol 266 (6) ◽  
pp. C1544-C1551 ◽  
Author(s):  
R. A. Khalil ◽  
C. Lajoie ◽  
K. G. Morgan
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Second Messengers ◽  
In Vitro Assays ◽  
Intact Cells ◽  
Kinase C ◽  
Biochemical Assays

Because of inherent difficulties in maintaining physiological conditions in biochemical assays, the intracellular free Ca2+ concentration ([Ca2+]i) required for activation of protein kinase C (PKC) in intact cells remains unclear. In the present study, [Ca2+]i was measured in freshly isolated vascular smooth muscle cells loaded with fura 2 while, in parallel, the distribution of the Ca(2+)-dependent alpha-PKC isoform was monitored using digital imaging microscopy. The [Ca2+]i alpha-PKC translocation threshold was determined by changing extracellular free Ca2+ concentration in steps while monitoring [Ca2+]i. In the absence of agonists, increasing [Ca2+]i caused < 25% of maximal translocation. In the presence of phenylephrine, maximum translocation occurred at [Ca2+]i > or = 198 nM. Phenylephrine augmented translocation of alpha-PKC primarily by increasing the slope of the [Ca2+]i-PKC translocation relationship. These results indicate that the [Ca2+]i threshold of alpha-PKC translocation in situ is less than that reported in most in vitro assays and are consistent with an effect of agonist-induced generation of other second messengers that cause cooperative interactions leading to translocation.

Detection and mapping of interactions between chromatin and the nuclear envelope in drosophila embryos

1995 ◽  
Vol 53 ◽  
pp. 764-765
Author(s):  
W.F. Marshall ◽  
A.F. Dernburg ◽  
B. Harmon ◽  
J.W. Sedat
Keyword(s):  
Nuclear Envelope ◽  
Chromatin Condensation ◽  
Three Dimensional ◽  
Physiological Role ◽  
Nuclear Surface ◽  
Intact Cells ◽  
Molecular Determinants ◽  
Surface Harmonic ◽  
Drosophila Embryos

Interactions between chromatin and nuclear envelope (NE) have been implicated in chromatin condensation, gene regulation, nuclear reassembly, and organization of chromosomes within the nucleus. To further investigate the physiological role played by such interactions, it will be necessary to determine which loci specifically interact with the nuclear envelope. This will not only facilitate identification of the molecular determinants of this interaction, but will also allow manipulation of the pattern of chromatin-NE interactions to probe possible functions. We have developed a microscopic approach to detect and map chromatin-NE interactions inside intact cells.Fluorescence in situ hybridization (FISH) is used to localize specific chromosomal regions within the nucleus of Drosophila embryos and anti-lamin immunofluorescence is used to detect the nuclear envelope. Widefield deconvolution microscopy is then used to obtain a three-dimensional image of the sample (Fig. 1). The nuclear surface is represented by a surface-harmonic expansion (Fig 2). A statistical test for association of the FISH spot with the surface is then performed.

SEX DETERMINATION OF FETUS PRENATAL FROM MATERNAL PLASMA BY USING DUPLEX PCR TECHNIQUE IN GOAT

2014 ◽  
Vol 13 (1) ◽  
pp. 50-59
Author(s):  
A NisreenYasirJasim ◽  
Tahir A. Fahid ◽  
Talib Ahmed Jaayid
Keyword(s):  
Sex Determination ◽  
Maternal Plasma ◽  
Duplex Pcr

INDIREKTE PAPIERCHROMATOGRAPHISCHE METHODE ZUR BESTIMMUNG DER HARNOESTROGENE

1961 ◽  
Vol 38 (4) ◽  
pp. 545-562 ◽  
Author(s):  
L. Kecskés ◽  
F. Mutschler ◽  
I. Glós ◽  
E. Thán ◽  
I. Farkas ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Granulosa Cell ◽  
Iron Content ◽  
List Type ◽  
Granulosa Cell Tumour ◽  
Hydrolysis Of ◽  
Phenol Fraction ◽  
Qualitative Determination

ABSTRACT 1. An indirect paperchromatographic method is described for separating urinary oestrogens; this consists of the following steps: acidic hydrolysis, extraction with ether, dissociation of phenol-fractions with partition between the solvents. Previous purification of phenol fraction with the aid of paperchromatography. The elution of oestrogen containing fractions is followed by acetylation. Oestrogen acetate is isolated by re-chromatography. The chromatogram was developed after hydrolysis of the oestrogens 'in situ' on the paper. The quantity of oestrogens was determined indirectly, by means of an iron-reaction, after the elution of the iron content of the oestrogen spot, which was developed by the Jellinek-reaction. 2. The method described above is satisfactory for determining urinary oestrogen, 17β-oestradiol and oestriol, but could include 16-epioestriol and other oestrogenic metabolites. 3. The sensitivity of the method is 1.3–1.6 μg/24 hours. 4. The quantitative and qualitative determination of urinary oestrogens with the above mentioned method was performed in 50 pregnant and 9 non pregnant women, and also in 2 patients with granulosa cell tumour.

Study of Significance of Pelvic Brim Chord and Pelvic Brim Depth in Sex Determination of Human Hip Bone

2012 ◽  
Vol 3 (1) ◽  
pp. 384-386
Author(s):  
Dr. Sudarshan Gupta ◽  
◽  
Dr. Bhadresh Vaghela ◽  
Dr. Deepak Howale ◽  
Dr. Mehul Tandel
Keyword(s):  
Sex Determination ◽  
Pelvic Brim
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