scholarly journals Interleukin 10 in Helicobacter pylori associated gastritis: immunohistochemical localisation and in vitro effects on cytokine secretion

2001 ◽  
Vol 54 (4) ◽  
pp. 285-292 ◽  
Author(s):  
K Bodger
Keyword(s):  
Helicobacter Pylori ◽  
Interleukin 10 ◽  
Cytokine Secretion ◽  
In Vitro Effects

scholarly journals Helicobacter pylori-Induced Disruption of Monolayer Permeability and Proinflammatory Cytokine Secretion in Polarized Human Gastric Epithelial Cells

2013 ◽  
Vol 81 (3) ◽  
pp. 876-883 ◽  
Author(s):  
Maria Fiorentino ◽  
Hua Ding ◽  
Thomas G. Blanchard ◽  
Steven J. Czinn ◽  
Marcelo B. Sztein ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Tight Junction ◽  
Proinflammatory Cytokines ◽  
Barrier Function ◽  
Cytokine Secretion ◽  
Gastric Epithelial Cells ◽  
Content Type ◽  
Monolayer Permeability ◽  
H Pylori

ABSTRACTHelicobacter pyloriinfection of the stomach is related to the development of diverse gastric pathologies. The ability ofH. pylorito compromise epithelial junctional complexes and to induce proinflammatory cytokines is believed to contribute to pathogenesis. The purpose of this study was to use anin vitrohuman gastric epithelial model to investigate the ability ofH. pylorito affect permeability and the extent and polarity of the host inflammatory response. NCI-N87 monolayers were cocultured with live or heat-killedH. pylorior culture supernatants. Epithelial barrier function was measured by transepithelial electric resistance (TEER) analysis, diffusion of fluorescein isothiocyanate (FITC)-labeled markers, and immunostaining for tight junction proteins. Supernatants from both apical and basolateral chambers were tested for cytokine production by multiplex analysis.H. pyloricaused a significant decrease in TEER, an increased passage of markers through the infected monolayer, and severe disruption and mislocalization of ZO-1 and claudin-1 proteins. Cell viability was not altered byH. pylori, indicating that loss of barrier function could be attributed to a breakdown of tight junction integrity. Significantly high levels of cytokine secretion were induced by either viable or heat-killedH. pylori.H. pyloriaffects monolayer permeability of polarized human gastric epithelial cells. Proinflammatory cytokines were secreted in a polarized manner, mostly basolaterally. Live bacteria are required for disruption of tight junctions but not for the induction of cytokine secretion. The NCI-N87 cell line provides an excellent model for thein vitrostudy ofH. pyloripathogenesis and the epithelial cell host response to infection.

scholarly journals In Vitro Effects of Lactobacillus plantarum LN66 and Antibiotics Used Alone or in Combination on Helicobacter pylori Mature Biofilm

2021 ◽  
Vol 9 (2) ◽  
pp. 424
Author(s):  
Jianfu Ji ◽  
Hong Yang
Keyword(s):  
Helicobacter Pylori ◽  
Antibiotic Therapy ◽  
Lactobacillus Plantarum ◽  
Laser Scanning ◽  
Extracellular Polymeric Substances ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
In Vitro Effects ◽  
H Pylori

Helicobacter pylori is a gastrointestinal pathogen with high prevalence that harms human health. Studies have shown that H. pylori can form antibiotic-tolerant biofilms, which may interfere with the efficacy of clinical antibiotic therapy. Probiotics can antagonize planktonic and biofilm pathogen cells and thus may play an auxiliary role in H. pylori antibiotic therapy. However, the effects of different probiotic strains and antibiotic combinations on H. pylori biofilms need to be further investigated. We determined the cell viability of H. pylori mature biofilms after treatment with Lactobacillus plantarum LN66 cell-free supernatant (CFS), clarithromycin (CLR), and levofloxacin (LVX) alone or in combination by the XTT method. Biofilm cells were observed by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Subsequently, protein and polysaccharide concentrations in biofilm extracellular polymeric substances (EPSs) were quantitatively detected by the Bradford method and the phenol-sulfate method. The results showed that LN66 CFS had an eradication effect on mature H. pylori biofilm. When used in combination with CLR, LN66 CFS significantly attenuated the eradication effect of CLR on biofilms; in contrast, when used in combination with LVX, LN66 CFS enhanced the disrupting effect of LVX. We speculate that the different effects of CFS and antibiotic combinations on biofilms may be related to changes in the content of proteins and polysaccharides in EPS and that the combination of CFS and CLR might promote the secretion of EPS, while the combination of CFS and LVX might have the opposite effect. Accordingly, we suggest that supplementation with L. plantarum LN66 may provide additional help when therapy involving LVX is used for clinical H. pylori biofilm eradication, whereas it may impair CLR efficacy when therapy involving CLR is used.

scholarly journals In Vitro Effects of Vitamins C and E on Helicobacter pylori

2021 ◽  
Vol 2 (3) ◽  
pp. 102-106
Author(s):  
Muhammed Kaya ◽  
Alpaslan Tanoğlu ◽  
Züleyha Akkan Çetinkaya ◽  
Efe Serkan Boz ◽  
Hayrunnisa Sezikli ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
In Vitro Effects

scholarly journals Transfer of Antigen-Pulsed Dendritic Cells Induces Specific T-Cell Proliferation and a Therapeutic Effect against Long-Term Helicobacter pylori Infection in Mice

2006 ◽  
Vol 74 (2) ◽  
pp. 984-993 ◽  
Author(s):  
Satoshi Otsu ◽  
Kazuyo Gotoh ◽  
Tetsu Yamashiro ◽  
Junpei Yamagata ◽  
Kouichirou Shin ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Interleukin 10 ◽  
Cell Mediated Immunity ◽  
Mouse Bone Marrow ◽  
Peptic Ulcers ◽  
Th1 Cell ◽  
Necrosis Factor Alpha ◽  
Ifn Γ ◽  
H Pylori

ABSTRACT Helicobacter pylori causes persistent infection of the stomach and results in chronic gastritis and peptic ulcers. Jaws II cells, derived from mouse bone marrow, were pulsed with live or formalin-killed or whole-cell sonicates (WCS) of H. pylori. Representative cell surface molecules were expressed at substantial levels on Jaws II cells, indicating that appropriate maturation of the cells was achieved with the three H. pylori antigens without any significant differences. H. pylori WCS-pulsed Jaws II cells secreted a significant amount of tumor necrosis factor alpha into the culture supernatant. The naïve T cells exposed to the WCS-pulsed Jaws II cells showed significant proliferation and gamma interferon (IFN-γ) and interleukin-10 (IL-10) production in vitro. A 2-log reduction in the number of colonizing bacteria was observed in the mice treated with the WCS-pulsed Jaws II cells; however, no significant reductions were achieved in mice treated with Jaws II cells pulsed with other H. pylori antigens. Up-regulated production of IFN-γ and IL-10 was observed in the stomachs of the mice treated with the WCS-pulsed Jaws II cells, which is consistent with the result obtained in vitro. There were no differences in gastritis scores or H. pylori-specific antibody titers among the mice treated with Jaws II cells pulsed with the three different H. pylori antigens. The results suggest that Th1 cell-mediated immunity in combination with Th2 cell-mediated immunity plays a role in reducing colonizing bacterial numbers in mice with chronic H. pylori infections.

scholarly journals Consistent production of a higher TH1:TH2 cytokine ratio by stimulated T cells in men compared with women

2000 ◽  
pp. 31-36 ◽  
Author(s):  
JA Giron-Gonzalez ◽  
FJ Moral ◽  
J Elvira ◽  
D Garcia-Gil ◽  
F Guerrero ◽  
...  
Keyword(s):  
Sex Hormones ◽  
Interleukin 2 ◽  
Interleukin 10 ◽  
Lymphocyte Culture ◽  
Interleukin 4 ◽  
T Helper ◽  
T Helper 1 ◽  
In Vitro Effects ◽  
Culture Supernatants

OBJECTIVE: To evaluate the T helper 1 (T(H)1)/T helper 2 (T(H)2) lymphocyte cytokine profiles in women and men and to study the in vitro effects of sex hormones on lymphocyte secretion of cytokines. METHODS: Analysis of serum concentration and lymphocyte synthesis of T(H)1 (gamma interferon (INF-gamma) and interleukin 2 (IL-2)) and T(H)2 (interleukin 4 (IL-4) and interleukin 10 (IL-10)) cytokines was performed in 20 women and 15 men. Analysis of modifications in cytokine secretion induced by supplementation of lymphocyte culture with increasing concentrations of sex hormones was carried out. RESULTS: Higher levels of INF-gamma and IL-2 and lower levels of IL-4 and IL-10 were detected in the phytohemagglutinin-stimulated lymphocyte culture supernatants of men compared with women; the INF-gamma:IL-4 ratio was significantly higher in men. In women, similar concentrations of all the cytokines were detected in culture supernatants obtained during the follicular and the luteal phases. The addition of sex hormones did not modify the concentration of cytokines in supernatants of phytohemagglutinin-stimulated T-cell cultures. CONCLUSIONS: Women present a predominant T(H)2 cytokine profile, which could be involved in immune responses characterized principally by the secretion of antibodies. This could be a factor implicated in the higher concentration of immunoglobulins or the increased prevalence of autoimmune diseases detected in females.

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