Development of a test for bovine tuberculosis in cattle based on measurement of gamma interferon mRNA by real-time PCR

2013 ◽  
Vol 173 (5) ◽  
pp. 117-117 ◽  
Author(s):  
W. Gan ◽  
X. Zhou ◽  
H. Yang ◽  
H. Chen ◽  
J. Qiao ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Tuberculosis ◽  
Gamma Interferon

scholarly journals Development and Evaluation of a Test for Tuberculosis in Live European Badgers (Meles meles) Based on Measurement of Gamma Interferon mRNA by Real-Time PCR

2007 ◽  
Vol 45 (8) ◽  
pp. 2398-2403 ◽  
Author(s):  
J. Sawyer ◽  
D. Mealing ◽  
D. Dalley ◽  
D. Dave ◽  
S. Lesellier ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Meles Meles ◽  
Gamma Interferon ◽  
European Badgers

scholarly journals Nasal swab real-time PCR is not suitable for in vivo diagnosis of bovine tuberculosis

2017 ◽  
Vol 37 (6) ◽  
pp. 549-554
Author(s):  
Fabiana Q. Mayer ◽  
Emily M. dos Reis ◽  
André Vinícius A. Bezerra ◽  
Rogério O. Rodrigues ◽  
Thais Michel ◽  
...  
Keyword(s):  
Real Time ◽  
Sensitivity And Specificity ◽  
Real Time Pcr ◽  
Bovine Tuberculosis ◽  
Tuberculin Test ◽  
Disease Diagnosis ◽  
Economic Losses ◽  
Nasal Swabs ◽  
In Vivo Diagnosis

ABSTRACT: Bovine tuberculosis (bTB) is a zoonosis causing economic losses and public health risks in many countries. The disease diagnosis in live animals is performed by intradermal tuberculin test, which is based on delayed hypersensitivity reactions. As tuberculosis has complex immune response, this test has limitations in sensitivity and specificity. This study sought to test an alternative approach for in vivo diagnosis of bovine tuberculosis, based on real-time polymerase chain reaction (PCR). DNA samples, extracted from nasal swabs of live cows, were used for SYBR® Green real-time PCR, which is able to differentiate between Mycobacterium tuberculosis and Mycobacterium avium complexes. Statistical analysis was performed to compare the results of tuberculin test, the in vivo gold standard bTB diagnosis method, with real-time PCR, thereby determining the specificity and sensitivity of molecular method. Cervical comparative test (CCT) was performed in 238 animals, of which 193 had suitable DNA from nasal swabs for molecular analysis, as indicated by amplification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, and were included in the study. In total, 25 (10.5%) of the animals were CCT reactive, of which none was positive in the molecular test. Of the 168 CCT negative animals, four were positive for M. tuberculosis complex at real time PCR from nasal swabs. The comparison of these results generated values of sensitivity and specificity of 0% and 97.6%, respectively; moreover, low coefficients of agreement and correlation (-0.029 and -0.049, respectively) between the results obtained with both tests were also observed. This study showed that real-time PCR from nasal swabs is not suitable for in vivo diagnosis of bovine tuberculosis; thus tuberculin skin test is still the best option for this purpose.

Real-Time PCR Detection of Mycobacterium bovis in Blood and Lymph Node Aspirates of Bovines Positive in Tuberculosis Screening Tests

2020 ◽  
Author(s):  
D. Narang ◽  
G. S. Sidhu ◽  
G. Filia ◽  
A. Singh ◽  
S. T. Singh ◽  
...  
Keyword(s):  
Lymph Node ◽  
Real Time ◽  
Mycobacterium Bovis ◽  
Real Time Pcr ◽  
Bovine Tuberculosis ◽  
Tuberculin Test ◽  
Screening Tests ◽  
Low Molecular Weight ◽  
Tuberculosis Screening ◽  
Three Samples

Bovine tuberculosis is a chronic infectious disease affecting broad range of mammalian hosts. ESAT-6 is a low molecular weight immunodominant protein coded by esxA gene located on RD1 region of genome and is responsible for virulence of Mycobacterium bovis. Out of 200 animals screened for bovine Tuberculosis (bTB), 38 animals (19%) were found positive for Comparative intradermal tuberculin test (CITT) (32 cattle, 6 buffaloes) and 41 animals were tested positive by IFN-g assay (29 cattle, 12 buffaloes). DNA extraction of blood (n=200) and lymph node aspirates (n=48) was done. Among 200 blood samples targeted for esxA (ESAT-6) gene, three samples (1.5%) whose CT was between 23-34 were considered positive by real-time PCR. Out of 48 animals (lymph node aspirates) that were positive either by CITT or IFN-g, one sample (2.08%) whose CT was between 23-34 were considered positive by real-time PCR. Remaining samples whose CT values were equal to or greater than 35 were considered negative. The sensitivity of esxA was 8 pg/ìl by real time PCR.

Feldvalidierung einer real-time PCR zum Nachweis von Mycoplasma hyopneumoniae in Nasentupfermaterial von lebenden Schweinen

2005 ◽  
Vol 147 (9) ◽  
pp. 373-379 ◽  
Author(s):  
F. Zeeh ◽  
P. Kuhnert ◽  
R. Miserez ◽  
M. G. Doherr ◽  
W. Zimmermann
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Mycoplasma Hyopneumoniae

Development of a novel real-time PCR assay for the quantitation of HBV DNA

2001 ◽  
Vol 34 (0) ◽  
pp. 129
Author(s):  
D Paraskevis
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Hbv Dna ◽  
Pcr Assay

Nutzen des hoch-sensitiven real-time-PCR HBV Tests (TaqMan) zur Prädiktion der Resistenzentwicklung bei Lamivudin-Langzeittherapie

2010 ◽  
Vol 48 (08) ◽  
Author(s):  
A Brodzinski ◽  
F van Bömmel ◽  
B Fülöp ◽  
B Schlosser ◽  
M Biermer ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr

Nachweis des porzinen Circovirus Typ 2 und des Torque-Teno-Sus-Virus 1 und 2 in Samenproben von Ebern einer österreichischen Besamungsstation

2011 ◽  
Vol 39 (04) ◽  
pp. 201-204
Author(s):  
A. Griessler ◽  
E. Pirker ◽  
H. Söllner ◽  
J. Segalés ◽  
T. Kekarainen ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Klinische Relevanz ◽  
Torque Teno Sus Virus

Zusammenfassung Gegenstand und Ziel: Das porzine Circovirus Typ 2 (PCV-2) und das Torque-teno-Sus-Virus (TTSuV) sind in schweineproduzierenden Ländern häufig nachzuweisen. Beide Erreger können sowohl horizontal als auch vertikal übertragen werden und Ebersamen könnte ein wichtiges Übertragungsmedium darstellen. Ziel der Studie war die Abklärung der Prävalenz dieser beiden Viren in Samenproben von Ebern. Material und Methoden: Von 100 Ebern einer Besamungsstation wurde jeweils eine Samenprobe mittels quantitativer Real-Time-PCR auf PCV-2 und mittels konventioneller PCR auf TTSuV-1 und TTSuV-2 untersucht. Ergebnisse: Nur bei einem Eber der Rasse Piétrain war ein positives PCV-2-Resultat festzustellen. TTSuV-1 ließ sich in vier Samenproben, TTSuV-2 in fünf Proben nachweisen. Ein Eber wies eine Koinfektion mit beiden TTSuV-Genotypen auf. Alle TTSuV-positiven Proben stammten von Piétrain-Ebern. Schlussfolgerung und klinische Relevanz: In der vorliegenden Studie wurde erstmals in Österreich TTSuV im Samen nachgewiesen. Die Prävalenz sowohl von TTSuV als auch von PCV-2 war gering. Die klinische Relevanz einer gleichzeitigen Kontamination des Samens mit beiden Viren ist nicht klar.

Genexpressionsanalyse an Tiermodellen des akuten neonatalen Lungenversagens: Evaluation dreier potentieller Housekeeping-Gene für die Real-Time PCR

2004 ◽  
Vol 208 (S 1) ◽  
Author(s):  
D Rawer ◽  
A Hilgendorff ◽  
M Dörner ◽  
A Trotter ◽  
J Leick ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Housekeeping Gene

Schnellnachweis von Campylobacter jejuni und Campylobacter coli aus Lebensmittelvoranreicherungen mittels Real-Time-PCR sowie immunomagnetischer Separation

2005 ◽  
Vol 67 (03) ◽  
Author(s):  
A Mayr ◽  
H Söllner ◽  
R Zucker ◽  
A Wieland ◽  
A Notzon ◽  
...  
Keyword(s):  
Real Time ◽  
Campylobacter Jejuni ◽  
Real Time Pcr ◽  
Campylobacter Coli

Vergleich von Ovarialendometriose, Ovarialkarzinom und benignen Ovarien mittels Microarray-Untersuchungen und Real-time-PCR

2005 ◽  
Vol 66 (S 1) ◽  
Author(s):  
D Hornung ◽  
C Banz ◽  
U Ungethüm ◽  
RJ Kuban ◽  
H Xu ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr
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