Sandwich ELISA detection of Mycoplasma bovis in pneumonic calf lungs and nasal swabs

1994 ◽  
Vol 135 (22) ◽  
pp. 531-532 ◽  
Author(s):  
H. Ball ◽  
D. Finlay ◽  
G. Reilly
Keyword(s):  
Sandwich Elisa ◽  
Mycoplasma Bovis ◽  
Nasal Swabs

scholarly journals Prevalence of pathogens from Mollicutes class in cattle affected by respiratory diseases and molecular characteristics of Mycoplasma bovis field strains

2016 ◽  
Vol 60 (4) ◽  
pp. 391-397 ◽  
Author(s):  
Ewelina Szacawa ◽  
Monika Szymańska-Czerwińska ◽  
Krzysztof Niemczuk ◽  
Katarzyna Dudek ◽  
Grzegorz Woźniakowski ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Respiratory Diseases ◽  
Molecular Characteristics ◽  
Mycoplasma Bovis ◽  
Gene Fragment ◽  
Nasal Swabs ◽  
Pcr Products ◽  
Cattle Herds ◽  
Ureaplasma Diversum ◽  
Mycoplasma Spp

Abstract Introduction: Mycoplasma bovis is one of the main pathogens involved in cattle pneumonia. Other mycoplasmas have also been directly implicated in respiratory diseases in cattle. The prevalence of different Mycoplasma spp. in cattle affected by respiratory diseases and molecular characteristics of M. bovis field strains were evaluated. Material and Methods: In total, 713 nasal swabs from 73 cattle herds were tested. The uvrC gene fragment was amplified by PCR and PCR products were sequenced. PCR/DGGE and RAPD were performed. Results: It was found that 39 (5.5%) samples were positive for M. bovis in the PCR and six field strains had point nucleotide mutations. Additionally, the phylogenetic analysis of 20 M. bovis field strains tested with RAPD showed two distinct groups of M. bovis strains sharing only 3.8% similarity. PCR/DGGE analysis demonstrated the presence of bacteria belonging to the Mollicutes class in 79.1% of DNA isolates. The isolates were identified as: Mycoplasma bovirhinis, M. dispar, M. bovis, M. canis, M. arginini, M. canadense, M. bovoculi, M. alkalescens, and Ureaplasma diversum. Conclusion: Different Mycoplasma spp. strains play a crucial role in inducing respiratory diseases in cattle.

scholarly journals PRESENCE OF MOLLICUTES AND MYCOPLASMA BOVIS IN NASAL SWABS FROM CALVES AND IN MILK FROM COWS WITH CLINICAL MASTITIS

2021 ◽  
Vol 28 ◽  
pp. 1-9
Author(s):  
Brunna Mayla Vasconcelos Adorno ◽  
Anelise Salina ◽  
Sâmea Fernandes Joaquim ◽  
Felipe De Freitas Guimarães ◽  
Bruna Churocof Lopes ◽  
...  
Keyword(s):  
Respiratory Diseases ◽  
Nasal Swab ◽  
Molecular Detection ◽  
Opportunistic Pathogen ◽  
Clinical Mastitis ◽  
Mycoplasma Bovis ◽  
Chain Reaction ◽  
Milk Samples ◽  
Nasal Swabs ◽  
Polymerase Chain

Mycoplasma bovis is part of the bovine respiratory tract microbiota but is considered an opportunistic pathogen of extreme importance in respiratory diseases of calves. It causes to the herd several diseases such as mastitis, polyarthritis, pneumonia and endometritis. This pathogen is highly contagious and animals with mastitis are potential disseminators of infection to the herd since they release from 106 to 108 CFU per mL milk. Similarly, animals with pneumonia eliminate, through respiratory secretions, high microbial loads of the agent. The present study aimed to perform molecular detection of Mycoplasma bovis in 185 milk samples from cows with clinical mastitis, as well as in 50 nasal swab samples from healthy calves with or without signs of pneumonia and born from cows with mastitis, all belonging to four dairy farms in Paraná State, where cases of mastitis had beendiagnosed. DNA extraction from both secretions was carried out according to the thermolysis method. For polymerase chain reaction (PCR), generic primers were employed to amplify the Mollicutes DNA and positive samples were subjected to PCR with primersspecific for M. bovis. Positivity for M. bovis was 3.78% in milk samples, regardless of the farm, and 20% in nasal swabs.

F Gene and N Gene based Reverse Transcription PCR for Molecular Characterization of Peste des Petits Ruminants Virus

2020 ◽  
Author(s):  
A K Pandey ◽  
D M Muglikar ◽  
P P Mhase ◽  
M M Pawade ◽  
S N Daphal ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Sandwich Elisa ◽  
Small Ruminants ◽  
N Gene ◽  
Tissue Samples ◽  
F Gene ◽  
Specific Pcr ◽  
Percent Positivity ◽  
Nasal Swabs ◽  
Ppr Virus

Present investigation was undertaken to detect and characterize the PPR virus from different clinical tissue samples of 14 sheep and 17 goats with respiratory disease from Maharashtra, India. All animals were tested by Sandwich ELISA, of which 70.96% were found positive carrying high PPR virus inwhich 12 were sheep and 10 were goats respectively. For confirmation of PPR, molecular detection was performed with RT-PCR using F gene and N gene specific primers. Intestine samples accounted for highest percent positivity (75%) followed by blood (66.66%) and lymph node (62.5%) for presence of virus. Unusually higher positivity was observed in heart, liver and Kidney (60%, each) than normal predilection sites such as lungs (57.84%) and spleen (50%). While nasal swabs and blood were individually processed with F gene and N gene specific PCR, the triturated organs were pooled for processing into ‘Sample A’ comprised of heart, kidney, liver and intestine combined together and ‘Sample B’ comprising of lung, spleen and lymph nodes combined for the molecular detection of PPR yielding the products each of 372bp and 463bp sizes, respectively. Out of total 40 samples tested, 09/12 each from both sample A and B, while 02/10 nasal swabs resulted positive, respectively and all 06 blood samples remained negative. (F as well as N gene PCR methods were found best suitable for detection of PPR virus from tissue samples of small ruminants).

scholarly journals Immunomodulatory effect of Mycoplasma bovis in experimentally infected calves

2013 ◽  
Vol 57 (4) ◽  
pp. 499-506 ◽  
Author(s):  
Katarzyna Dudek ◽  
Dariusz Bednarek ◽  
Roger D. Ayling ◽  
Ewelina Szacawa
Keyword(s):  
Acute Phase Proteins ◽  
Physiological Saline ◽  
Interferon Γ ◽  
Mucosal Immune Response ◽  
Interleukin 4 ◽  
Control Group ◽  
Mycoplasma Bovis ◽  
Immunological Parameters ◽  
Amyloid A ◽  
Nasal Swabs

Abstract The effect of three different field isolates of Mycoplasma bovis on selected immunological parameters in experimentally infected calves was studied. Calves were kept separately in 4 experimental groups, and animals of 3 groups were infected intratracheally with one of the three selected isolates of M. bovis. The control group was inoculated intra-tracheally with sterile physiological saline. Nasal swabs and blood samples were collected just before the calf inoculation, then daily for seven days, and then weekly for another three weeks. The presence of M. bovis antigen, M. bovis antibodies, total protein, gamma globulins, IgA, IgM, IgG, acute phase proteins (haptoglobulin and serum amyloid A), as well as interferon-γ and interleukin-4 concentrations were determined. M. bovis was detected intermittently during the study in the infected groups from day 1, whilst the control group remained free of the pathogen. M. bovis antibodies were detected in some of infected animals in the second, third, and fourth week after infection. The stimulation and/or immunological suppression varied between the M. bovis isolates used for the inoculation. All M. bovis isolates induced a rise of APP and gamma globulin concentrations in infected calves. However, in this study the mucosal immune response appeared to be down-regulated, which was expressed with a general lack of IgA stimulation.

scholarly journals Protein and Antigenic Profile among Mycoplasma bovis Field Strains Isolated in Bosnia and Herzegovina

2009 ◽  
Vol 78 (1) ◽  
pp. 151-154 ◽  
Author(s):  
Maid Rifatbegović ◽  
Patrícia Assunção ◽  
Šemso Pašić ◽  
Christian de la Fe ◽  
Jose B. Poveda
Keyword(s):  
Bosnia And Herzegovina ◽  
Gel Electrophoresis ◽  
Reference Strain ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Geographic Region ◽  
Mycoplasma Bovis ◽  
Nasal Swabs ◽  
Geographic Regions ◽  
Antigenic Profile

Mycoplasma bovis is a serious, worldwide-spread but often overlooked pathogen causing respiratory disease, mastitis, and arthritis in cattle. In this study we characterize the protein and antigenic profiles of M. bovis field strains isolated in Bosnia and Herzegovina by sodium dodecyl sulphate polyacrylamide gel electrophoresis and immunoblotting, and analyze possible variations among these strains. Greater differences occurred when comparing field strains with the reference strain PG45. One field strain isolated from lung samples of a heifer was markedly different from strains isolated from nasal swabs taken from cattle raised in another geographic region. A possible correlation may exist between protein and antigen profiles of M. bovis field strains, geographic regions and anatomical sites of isolation.

scholarly journals Monitoring Mycoplasma bovis Diversity and Antimicrobial Susceptibility in Calf Feedlots Undergoing a Respiratory Disease Outbreak

Pathogens ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 593 ◽  
Author(s):  
Claire A.M. Becker ◽  
Chloé Ambroset ◽  
Anthéa Huleux ◽  
Angélique Vialatte ◽  
Adélie Colin ◽  
...  
Keyword(s):  
Respiratory Diseases ◽  
Mycoplasma Bovis ◽  
Pfge Analysis ◽  
Bovine Respiratory Diseases ◽  
Veal Calf ◽  
Nasal Swabs ◽  
Relative Prevalence ◽  
Antimicrobial Treatments

Bovine respiratory diseases (BRD) are widespread in veal calf feedlots. Several pathogens are implicated, both viruses and bacteria, one of which, Mycoplasma bovis, is under-researched. This worldwide-distributed bacterium has been shown to be highly resistant in vitro to the main antimicrobials used to treat BRD. Our objective was to monitor the relative prevalence of M. bovis during BRD episodes, its diversity, and its resistance phenotype in relation to antimicrobial use. For this purpose, a two-year longitudinal follow-up of 25 feedlots was organized and 537 nasal swabs were collected on 358 veal calves at their arrival in the lot, at the BRD peak and 4 weeks after collective antimicrobial treatments. The presence of M. bovis was assessed by real-time PCR and culture. The clones isolated were then subtyped (polC subtyping and PFGE analysis), and their susceptibility to five antimicrobials was determined. The course of the disease and the antimicrobials used had no influence on the genetic diversity of the M. bovis strains: The subtype distribution was the same throughout the BRD episode and similar to that already described in France, with a major narrowly-variable subtype circulating, st2. The same conclusion holds for antimicrobial resistance (AMR) phenotypes: All the clones were already multiresistant to the main antimicrobials used (except for fluoroquinolones) prior to any treatments. By contrast, changes of AMR phenotypes could be suspected for Pasteurellaceae in two cases in relation to the treatments registered.

scholarly journals Isolation of Mycoplasma alkalescens and Mycoplasma bovis from the Nasal Swabs of Calves with Pneumonia

1994 ◽  
Vol 47 (5) ◽  
pp. 311-314 ◽  
Author(s):  
Ryuta KUDOU ◽  
Seiya HIROSHIMA ◽  
Keisuke TUKAHARA ◽  
Takashi SAKAMOTO ◽  
Takaaki HIRANO ◽  
...  
Keyword(s):  
Mycoplasma Bovis ◽  
Nasal Swabs

scholarly journals Last Survey of Mycoplasma Bovis Prevalence in Polish Cattle Affected with Respiratory Syndrome

2012 ◽  
Vol 56 (4) ◽  
pp. 447-451 ◽  
Author(s):  
Katarzyna Dudek ◽  
Dariusz Bednarek
Keyword(s):  
Mycoplasma Bovis ◽  
Serum Samples ◽  
North West ◽  
The North ◽  
East Region ◽  
West Region ◽  
South West ◽  
Nasal Swabs ◽  
The Mean ◽  
South West Region

Abstract The aim of the study was to determine the prevalence of Mycoplasma bovis infection in clinical cases of bovine respiratory disease (BRD) in 2011. There were examined 841 serum samples and 41 nasal swabs and lungs of cattle from different farms of six regions of Poland (13 provinces). The obtained results indicated that the mean prevalence of M. bovis infection in Polish cattle population suffering from BRD was 64.3% and among them 8.4% of the animals were highly positive. On the other hand, in particular regions, it altered - 72.0% in east region, 52.7% in central region, 66.4% in south region, 64.1% in north-west region, 56.5% in north region, and 58.8% in south-west region. It should be added that within the presented regions, the highest values (82.4%) were reported in south region (Malopolskie province), whereas the lowest ones (42.9%) were in east region (Lubelskie province). However, field strains of M. bovis were isolated from the lungs of affected cattle only in the north-west region of Poland (Zachodniopomorskie province).

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