Testing of bulk tank milk from Northern Ireland dairy herds for viral RNA and antibody to bovine viral diarrhoea virus

2001 ◽  
Vol 149 (9) ◽  
pp. 261-265 ◽  
Author(s):  
D. A. Graham ◽  
A. German ◽  
I. E. McLaren ◽  
D. A. Fitzpatrick
2013 ◽  
Vol 57 (2) ◽  
pp. 149-156 ◽  
Author(s):  
Aleksandra Kuta ◽  
Mirosław P. Polak ◽  
Magdalena Larska ◽  
Jan F. Żmudziński

Abstract The aim of the study was to evaluate the status of bovine viral diarrhoea virus (BVDV) infection in selected dairy herds in Poland with the use of commercial enzyme linked immonosorbent assay for the detection of specific antibodies (BVDV-Ab ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) for the detection of viral RNA, using bulk tank milk (BTM) samples. Two hundred and thirty-one samples of BTM were collected from 99 dairy herds in Poland. The herds were divided into four different classes according to the Swedish system of classification. The results showed that 70.7% of herds were BVDV antibodypositive. High levels of antibodies in 52.85 % (37 herds in class 3) of all antibody positive herds indicated acute BVDV infection. Thirty five samples with the highest antibody levels were tested by RT-PCR and five of them were positive for viral RNA. Dairy herds in Poland have high levels of antibodies against BVDV in BTM. Since no vaccination was implemented in the herds tested, high seroprevalence of BVDV antibodies in cattle indicates the widespread of BVDV infection in Polish cattle.


2014 ◽  
Vol 116 (1-2) ◽  
pp. 183-187
Author(s):  
Jolanta G. Rola ◽  
Magdalena Larska ◽  
Monika Grzeszuk ◽  
Lukasz Bocian ◽  
Aleksandra Kuta ◽  
...  

2012 ◽  
Vol 171 (18) ◽  
pp. 445-445 ◽  
Author(s):  
R. W. Humphry ◽  
F. Brülisauer ◽  
I. J. McKendrick ◽  
P. F. Nettleton ◽  
G. J. Gunn

2001 ◽  
Vol 92 (1) ◽  
pp. 19-22 ◽  
Author(s):  
Štefan Vilček ◽  
Ladislav Strojny ◽  
Branislav Ďurkovič ◽  
Wigbert Rossmanith ◽  
David Paton

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