Aflatoxins (B1, B2, G1 and G2) were surveyed in 417 beer samples purchased from the retail market in Spain (n=336), France (n=49), Portugal (n=15), Ireland (n=11) and Italy (n=6) in the period 2006-2012. In addition, 4 samples were acquired from Ghana and one from Israel. The analytical procedure was based on immunoaffinity clean-up and liquid chromatography with fluorescence detection. Aflatoxin was detected in 72.6% of the samples acquired in the Spanish market and in 182 out of 200 samples brewed in Spain with levels ranging from 0.08 to 36.12 ng/l for all four aflatoxins. The mean and median values of positive samples were estimated to be 3.51 and 2.07 ng/l, respectively, for samples acquired in Spain and 3.82 and 2.60 ng/l, respectively, for samples brewed in Spain. The mean and median values for the group of imported beers were 2.64 and 0.51 ng/l, respectively. Regarding the whole group of beers from European countries (417 samples), the incidence of positive samples was 64.7% with concentrations ranging from 0.07 to 45.18 ng/l for total aflatoxins with mean and median values of 3.47 and 1.82 ng/l, respectively. The overall median concentrations of aflatoxins in the samples of beers produced in Spain were 2.27-2.32 ng/l and for the whole group of European beers 0.43-0.62 ng/l (lower bound - upper bound). The median values obtained in this study for aflatoxin in beers consumed in Spain would result in an intake of approx. 97-112 pg/per capita/day, which represents a very small fraction (approx. 0.5% in a worst-case scenario) of the estimated average exposure to total aflatoxins.