The population structure of Armillaria ostoyae in the southern interior of British Columbia

The population structure of Armillaria ostoyae (Romagn.) Herink was investigated at a study site in the interior cedarhemlock zone of the southern interior of British Columbia. Eight 500-m long, randomly placed transects located at least 500 m apart were systematically sampled and individual genets were delineated using Random Amplified Polymorphic DNA (RAPD) analysis. Average genet intersect length was 79 m, and an estimated 88% of the area surveyed was occupied by genets <2 ha in size. The spatial distribution of genets in relation to one another was quite complex, with isolates of the same genet often non-contiguous along the transect. The location of genets could not be determined from aboveground symptom expression. Isolates collected from adjacent hosts within small groups of symptomatic hosts (infection foci) did not necessarily belong to the same genet, but single genets could occupy several infection foci. There was no relationship between geographic distance and genetic similarity of genets, suggesting random mating events for genet origin and long term maintenance of genetic identity.Key words: Armillaria, RAPD, population structure, genet, ramet, clone.

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The population structure of Armillaria ostoyae and Armillaria sinapina in the central interior of British Columbia

Canadian Journal of Botany ◽

10.1139/b01-033 ◽

2001 ◽

Vol 79 (5) ◽

pp. 600-611 ◽

Cited By ~ 3

Author(s):

Jeremy R Dettman ◽

Bart J van der Kamp

Keyword(s):

British Columbia ◽

Population Structure ◽

Reproductive Strategy ◽

Rapd Analysis ◽

Random Amplified Polymorphic Dna ◽

Somatic Incompatibility ◽

Armillaria Ostoyae ◽

Fungal Isolates ◽

Population Structures ◽

Do So

The population structures of Armillaria ostoyae (Romagn.) Herink and Armillaria sinapina Bérubé & Dessureault were investigated at a study site near Williams Lake in the central interior of British Columbia. One hundred and twenty eight fungal isolates were examined from nine infection centers and individual genets were delineated using random amplified polymorphic DNA (RAPD) analysis and somatic incompatibility tests. Six A. ostoyae genets ranging in size from 0.70 to >15 ha were detected. The population structure of A. ostoyae was consistent with a clonal reproductive strategy, and infection centers were occupied by single A. ostoyae genets or ramets thereof. Eighteen relatively small A. sinapina genets were detected, with infection centers being occupied by multiple genets. Armillaria sinapina appears to be more pathogenic to coniferous hosts than previously reported in the region. Armillaria ostoyae appears to initiate new infections of available substrate via airborne basidiospores at a lower frequency than A. sinapina. However once established, A. ostoyae can spread quite aggressively and capture significant amounts of secondary resources, while A. sinapina is unable to do so. The results of somatic incompatibility tests used to differentiate genets corresponded with the results of RAPD analysis, with only one minor discrepancy.Key words: Armillaria, RAPD, population structure, genet, ramet, clone.

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Detection of genetic diversity in rough fescue (Festuca campestris Rydb) populations of southern Alberta and British Columbia, Canada, using RAPD markers

Canadian Journal of Plant Science ◽

10.4141/cjps07071 ◽

2008 ◽

Vol 88 (2) ◽

pp. 307-312

Author(s):

Zhao Mengli ◽

Han Bing ◽

Walter D Willms

Keyword(s):

Genetic Diversity ◽

British Columbia ◽

Genetic Distance ◽

Random Amplified Polymorphic Dna ◽

Geographic Distance ◽

Genetic Distances ◽

Gene Frequencies ◽

Southern Alberta ◽

Potential Adaptation ◽

Rough Fescue

Mountain rough fescue (Festuca campestris Rydb.) is a tufted native grass in southern Alberta and British Columbia, Canada, and has attracted interest for use in reclamation. However, its seed is often available from only a few localized sources and may not be adapted for areas removed from the collection site. We conducted a study to determine the genetic variability among rough fescue populations to assess its potential adaptation. Thirty plants were collected from each of six populations and analyzed using Random Amplified Polymorphic DNA (RAPD). One population (Kamloops, BC) was separated by several mountain ranges from the five easterly Alberta populations.The Kamloops population was also separated from the Alberta populations by genetic distance in two clusters. Of the total genetic variation present in the data, 21% was found among populations while the remaining (79%) was found within populations. Nei’s genetic distances among populations were related to their geographical distances. Genetic differences among populations appeared to be caused primarily by differences in gene frequencies rather than rare genes. Also, genetic diversity appeared to increase from west to east suggesting that the more easterly populations had greater adaptation potential. We speculate that the more easterly populations are less likely to share genes since the prevailing winds are from the west. Germplasm from the more easterly populations may be used with suitable precautions within Alberta and possibly around Kamloops. Key words: Genetic distance, geographic distance, reclamation, potential adaptation

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Genetic variability in Indian populations of banana corm weevil [Cosmopolites sordidus (Coleoptera: Curculionidae)] assessed by RAPDs and AFLPs

International Journal of Tropical Insect Science ◽

10.1017/s1742758417000108 ◽

2017 ◽

Vol 37 (03) ◽

pp. 149-162 ◽

Cited By ~ 3

Author(s):

Sweta Kumari U. Yadav ◽

Jyotsna Singh ◽

B. Padmanaban ◽

Lalitha Sunil Kumar

Keyword(s):

Population Structure ◽

Gene Flow ◽

Hot Spot ◽

Random Amplified Polymorphic Dna ◽

Geographic Distance ◽

Mantel Test ◽

Cosmopolites Sordidus ◽

Restricted Gene Flow ◽

Control And Management ◽

Economic Pest

AbstractCosmopolites sordidus(Germar), commonly known as banana corm weevil, is an important economic pest in Asia that can cause severe yield loss depending upon the stage at which infestation occurs. In spite of its economic importance, little is known about the population structure of this pest in India. Random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) were used to characterize the population genetic structure ofC. sordiduscollected from five hot spot locations in India. Nineteen RAPD primers and five selective AFLP primer combinations generated 147 and 304 amplification products, respectively. UPGMA dendrograms generated with both marker systems failed to reveal populations clustered based on geographic distance, which was confirmed by the Mantel test, which did not show a strong correlation between genetic distance and geographic distance. Values of indices of genetic identity showed that the populations were closely related. Though the gene flow estimate (Nm) between the populations was 0.469, suggesting restricted gene flow, the populations are not genetically distinct. These observations suggest that the range expansion of this banana pest in India has taken place through transport of infested corms and plant material, resulting in genetically close populations that are geographically distinct. These results provide important information on the population structure of this pest in India, which will aid in designing suitable strategies for its control and management, especially with respect to insecticide resistance.

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The population structure of Armillaria ostoyae in the southern interior of British Columbia

Canadian Journal of Botany ◽

10.1139/cjb-79-5-612 ◽

2001 ◽

Vol 79 (5) ◽

pp. 612-620 ◽

Cited By ~ 4

Author(s):

Jeremy R. Dettman ◽

Bart J. van der Kamp

Keyword(s):

British Columbia ◽

Population Structure ◽

Armillaria Ostoyae

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Genetic Diversity Analysis of Elops machnata (Forskal) Populations in South East and West Coasts of India Using RAPD Markers

Notulae Scientia Biologicae ◽

10.15835/nsb649418 ◽

2014 ◽

Vol 6 (4) ◽

pp. 399-406

Author(s):

Vellaichamy RAMANADEVI ◽

Muthusamy THANGARAJ

Keyword(s):

Genetic Diversity ◽

Coastal Waters ◽

Rapd Markers ◽

South India ◽

Rapd Analysis ◽

Random Amplified Polymorphic Dna ◽

Geographic Distance ◽

Eastern Africa ◽

International Union ◽

South Indian

The Random Amplified Polymorphic DNA (RAPD) technique was used to study the genetic diversity of four Elops machnata populations in South India. Elops machnata is considered as a least concern species (LC), categorized by the International Union for Conservation and Nature (IUCN). The population trends are currently stable in Indian Ocean, Eastern Africa, but are unknown throughout the rest of its expansive range, especially in Indian estuaries. Among the ten RAPD primers tested, eight primers got amplified and gave scorable bands. In total, 119 scorable bands were observed in all populations. The overall observed and effective number of alleles was found to be 2.000 ± 0.000 and 1.5307 ± 0.2503 respectively for the entire population. The overall polymorphic loci were 61.00% and the overall gene flow among the four populations was predicted to 0.1032. The genetic distance and geographic distance between the four populations showed a positive correlation. The highest genetic similarity (0.6824) was found between Parangipettai and Muthupettai population, which reflected the geographical relationship between them. Tow main clusters were obtained based on UPGMA dendrogram. This study proves that RAPD analysis has the ability to discriminate E. machnata populations in South Indian coastal waters.

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RAPD variation in populations of Cardamine lilacina (Brassicaceae)

Australian Systematic Botany ◽

10.1071/sb9960291 ◽

1996 ◽

Vol 9 (3) ◽

pp. 291 ◽

Cited By ~ 12

Author(s):

MF Nolan ◽

ML Skotnicki ◽

AJ Gibbs

Keyword(s):

Population Structure ◽

Host Plant ◽

Rapd Analysis ◽

Genetic Relationships ◽

Random Amplified Polymorphic Dna ◽

Heterogeneous Population ◽

Natural Host ◽

Endemic Plant ◽

Plant Variation ◽

Recent Immigrant

Random amplified polymorphic DNA (RAPD) analysis of DNA samples clearly distinguished between Cardantine hirsuta L. and two variants of Cardamine lilacina Hook. found in Australia. Cardarnine hirsuta, a recent immigrant, was less variable than C. lilacina, robust alpine snow-patch variant (C. lilacina-RASPV), which is an endemic plant that grows in three distinct regions in the Kosciusko alpine area and C. lilacina, intermedia variant (C. lilacina-IV), which is an endemic plant that is widespread in the same area. Comparison of RAPDs showed that, in the populations of C. lilacina-RASPV, some plants found near each other were closely related, but there was no detectable largerscale population structure. It is most likely that the present populations of C. lilacina-RASPV are relics of a single larger heterogeneous population. Cardarnine lilacina-RASPV is the only known natural host of turnip yellow mosaic tymovirus in Australia. Although in this study RAPD analysis did not indicate whether the host plant variation is influencing the variability of populations of this virus it did provide an understanding of the genetic relationships in these populations of C. lilacina.

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The population structure of Armillaria ostoyae and Armillaria sinapina in the central interior of British Columbia

Canadian Journal of Botany ◽

10.1139/cjb-79-5-600 ◽

2001 ◽

Vol 79 (5) ◽

pp. 600-611 ◽

Cited By ~ 4

Author(s):

Jeremy R. Dettman ◽

Bart J. van der Kamp

Keyword(s):

British Columbia ◽

Population Structure ◽

Armillaria Ostoyae

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Characteristics and densities of Williamson’s Sapsucker nest trees in British Columbia

Canadian Journal of Forest Research ◽

10.1139/x09-138 ◽

2009 ◽

Vol 39 (12) ◽

pp. 2319-2331 ◽

Cited By ~ 6

Author(s):

L. W. Gyug ◽

C. Steeger ◽

I. Ohanjanian

Keyword(s):

British Columbia ◽

Endangered Species ◽

Populus Tremuloides ◽

Stand Structure ◽

Tree Density ◽

Trembling Aspen ◽

Larix Occidentalis ◽

Western Larch ◽

Term Maintenance

We characterized Williamson’s Sapsucker ( Sphyrapicus thyroideus (Cassin, 1852)) nest trees in southern British Columbia from 1995 to 2008 to determine the nesting requirements of this endangered species in Canada. In the East Kootenay (n = 32) and the Okanagan (n = 157) regions where western larch ( Larix occidentalis Nutt.) occurred, 81% and 75% of the nest trees were western larch, respectively. In regions west of the Okanagan Valley where western larch did not occur (n = 73), 77% of the nest trees were trembling aspen ( Populus tremuloides Michx.). Conifer nest trees were larger (72 cm DBH), on average, than broad-leaved nest trees (35 cm DBH), and this pattern was consistent across the breeding range of Williamson’s Sapsucker. Live western larch >67 cm DBH with either broken or dead tops were the trees with the highest frequency (20%) among the trees estimated to be suitable for nesting, primarily because these larch tended to be infected with heart rots. Based on observed densities, targets should be 1.36 suitable nest trees/ha in managed stands, although in many instances such as aspen patches, this target will only be met in portions of the nesting territories. The recommended stand structure for long-term maintenance of high nest-tree density in western larch stands is for ≥20 trees/ha with DBHs >57 cm and 60–150 trees/ha with DBHs >22 cm.

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Random Amplified Polymorphic DNA (RAPD) Analysis of Long-term Cultured Hybrid Hazelnut

HortScience ◽

10.21273/hortsci.39.5.1079 ◽

2004 ◽

Vol 39 (5) ◽

pp. 1079-1082 ◽

Cited By ~ 7

Author(s):

Mehmet Nuri Nas ◽

Nedim Mutlu ◽

Paul E. Read

Keyword(s):

Somaclonal Variation ◽

Clonal Propagation ◽

Rapd Analysis ◽

Random Amplified Polymorphic Dna ◽

Epigenetic Changes ◽

Phenotypic Analysis ◽

Micropropagated Plants ◽

Genetic Assessment

RAPD and phenotypic analysis were conducted to assess clonal stability of hazelnuts generated from axillary buds cultured in vitro for long-term. The nuts produced on in vitro-propagated plants were indistinguishable from those of donor plants. With the exception of rare horizontal (plagiotropic) growth, all in vitro-propagated plants exhibited phenotypes similar to those of donor plants. RAPD analysis did not reveal any somaclonal variation between donor plants from which in vitro cultures were initiated and micropropagated plants (6-year cultures), and no somaclonal variation was detected among in vitro-propagated plants. However, polymorphism (15.6%) was detected between the parent plant and its in vitro-propagated progenies (from seedlings). These results show a good discriminatory power of RAPD to detect polymorphism between samples where it is expected, and it can be effectively used for genetic assessment of micropropagated hazelnut. No evidence of genetic or epigenetic changes was observed in long-term cultured hazelnut, and thus long-term in vitro culture of hazelnut does not seem to limit its clonal propagation.

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