Effects of Buried Wood on the Development of Populus tremuloides on Various Oil Sands Reclamation Soils

Buried wood is an important but understudied component of reclamation soils. We examined the impacts of buried wood amounts and species on the growth of the common reclamation tree species trembling aspen (Populus tremuloides). In a greenhouse study, aspen seedlings were planted into four soil types, upland derived fine forest floor-mineral mix (fFFMM), coarse forest floor-mineral mix (cFFMM), and lowland derived peat and peat-mineral mix (PMM), that were mixed with either aspen or pine wood shavings at four concentrations (0%, 10%, 20% and 50% of total volume). Height and diameter growth, chlorophyll concentration, and leaf and stem biomass were measured. Soil nutrients and chemical properties were obtained from a parallel study. Buried wood primarily represents an input of carbon to the soil, increasing the C:N ratio, reducing the soil available nitrogen and potentially reducing plant growth. Soil type had the largest impact on aspen growth with fFFMM = peat > PMM > cFFMM. Buried wood type, i.e., aspen or pine, did not have an impact on aspen development, but the amount of buried wood did. In particular, there was an interaction between wood amount and soil type with a large reduction in aspen growth with wood additions of 10% and above on the more productive soils, but no reduction on the less productive soils.

Topographic Variation in Forest Expansion Processes across a Mosaic Landscape in Western Canada

Changes to historic fire and grazing regimes have been associated with the expansion of tree cover at forest–grassland boundaries. We evaluated forest expansion across a mosaic landscape in western Canada using aerial photos, airborne laser scanning, and field transects. The annual rate of forest expansion (0.12%) was on the low end of rates documented across North America and was greater from the 1970s to the 1990s than from the 1990s to 2018. Most forest expansion occurred within 50 m of established forests, and 68% of all tree regeneration in grasslands was within 15 m of the forest edge. The intensity of cattle grazing did not affect the tree regeneration density. Despite the slow pace of land cover change, grassland areas near the forest edge had an average of 20% canopy cover and 9 m canopy height, indicating the presence of tall but sporadic trees. The rate of forest expansion, density of tree regeneration, and tree cover within grasslands were all greater at lower elevations where trembling aspen (Populus tremuloides) and white spruce (Picea glauca) were the dominant tree species. We conclude that proportions of forest–grassland cover on this landscape are not expected to change dramatically in the absence of major fire over the next several decades.

Draft Genome Sequence of Mortierella alpina Strain LL118, Isolated from an Aspen (Populus tremuloides) Leaf Litter Sample

Mortierella alpina is a filamentous fungus commonly associated with soil and is one of very few fungal species known to include strains with ice nucleation activity. Here, we report the draft genome sequence of the ice nucleation-active M. alpina strain LL118, isolated from aspen leaf litter collected in Alberta, Canada.

Twenty-six years of aspen regeneration under varying light conditions in a boreal mixedwood forest

Density, height, and diameter at breast height of trembling aspen (Populus tremuloides Michx.) sucker regeneration were assessed over a 26-year period in openings created by harvesting in a 40-year-old aspen stand in northeastern Ontario (Canada). The opening types were 9- and 18-m diameter circles, 9- and 18- 150-m east-west strips, and a 100- 150-m clearcut, representing a range of light conditions. Density, height, and diameter at breast height of aspen regeneration were significantly affected by opening type, location relative to opening, and time since harvest. By year 26, aspen densities in circular openings declined to 0, despite high initial recruitment, and trembling aspen heights were significantly lower in the 9-m strips than in the 18-m strips or the clearcut. Year 26 aspen density, volume, and basal area increased with increasing initial light intensity, with the highest rate of increase between 80 and 100% light levels. Understory vegetation cover was largely unaffected by opening size; however, substantial understory aspen regeneration occurred in the smaller openings. Results support the traditional view that aspen is best managed under the clearcut silvicultural system, and >80% full light is recommended for adequate long-term aspen regeneration.

Genotypic variation rather than ploidy level shapes trait expression in a foundation tree species under drought stress and defoliation

With advancing climate change, tree survival increasingly depends on mechanisms that facilitate coping with multiple environmental stressors. At the population level, genetic diversity is a key determinant of a tree species’ capacity to deal with stress. However, little is known about the relative relevance of the different components of genetic diversity for shaping tree stress responses. We compared how two components of genetic diversity, genotypic variation and ploidy level, shape growth, phytochemical, and physiological traits of Populus tremuloides, under environmental stress. In two field experiments we exposed eight diploid and eight triploid aspen genotypes to individual and interactive drought stress and defoliation treatments. We found that: 1) Genotypic differences were critical for explaining variation of most of functional traits and their responses to stress. 2) Ploidy levels generally played a subordinate role for shaping traits, as they were typically obscured by genotypic differences. 3) As an exception to the second finding, we found that triploid trees expressed higher levels of foliar defenses, photosynthesis, and rubisco activity under well-watered conditions, and displayed greater drought resilience than diploids. This research demonstrates that the simultaneous study of multiple sources of genetic diversity is important for understanding how trees will respond to environmental change.

Twenty-six years of aspen regeneration under varying light conditions in a boreal mixedwood forest

Density, height, and diameter at breast height of trembling aspen (Populus tremuloides Michx.) sucker regeneration were assessed over a 26-year period in openings created by harvesting in a 40-year-old aspen stand in northeastern Ontario (Canada). The opening types were 9- and 18-m diameter circles, 9- and 18- _ 150-m east-west strips, and a 100- _ 150-m clearcut, representing a range of light conditions. Density, height, and diameter at breast height of aspen regeneration were significantly affected by opening type, location relative to opening, and time since harvest. By year 26, aspen densities in circular openings declined to 0, despite high initial recruitment, and trembling aspen heights were significantly lower in the 9-m strips than in the 18-m strips or the clearcut. Year 26 aspen density, volume, and basal area increased with increasing initial light intensity, with the highest rate of increase between 80 and 100% light levels. Understory vegetation cover was largely unaffected by opening size; however, substantial understory aspen regeneration occurred in the smaller openings. Results support the traditional view that aspen is best managed under the clearcut silvicultural system, and >80% full light is recommended for adequate long-term aspen regeneration.

Evaluating the Performance of a Forest Succession Model to Predict the Long-Term Dynamics of Tree Species in Mixed Boreal Forests Using Historical Data in Northern Ontario, Canada

Environmental concerns and economic pressures on forest ecosystems have led to the development of sustainable forest management practices. As a consequence, forest managers must evaluate the long-term effects of their management decisions on potential forest successional pathways. As changes in forest ecosystems occur very slowly, simulation models are logical and efficient tools to predict the patterns of forest growth and succession. However, as models are an imperfect representation of reality, it is desirable to evaluate them with historical long-term forest data. Using remeasured tree and stand data from three data sets from two ecoregions in northern Ontario, the succession gap model ZELIG-CFS was evaluated for mixed boreal forests composed of black spruce (Picea mariana [Mill.] B.S.P.), balsam fir (Abies balsamea [L.] Mill.), jack pine (Pinus banksiana L.), white spruce (Picea glauca [Moench] Voss), trembling aspen (Populus tremuloides Michx.), white birch (Betula papyrifera Marsh.), northern white cedar (Thuja occidentalis L.), American larch (Larix laricina [Du Roi] K. Koch), and balsam poplar (Populus balsamefera L.). The comparison of observed and predicted basal areas and stand densities indicated that ZELIG-CFS predicted the dynamics of most species consistently for periods varying between 5 and 57 simulation years. The patterns of forest succession observed in this study support gap phase dynamics at the plot scale and shade-tolerance complementarity hypotheses at the regional scale.

Intraspecific Variation In Plant Economic Traits Predicts Trembling Aspen Resistance To A Generalist Insect Herbivore

Patterns of trait expression within some plant species have recently been shown to follow patterns described by the leaf economics spectrum paradigm. Resistance to herbivores is also expected to covary with leaf economics traits. We selected multiple mature Populus tremuloides genotypes from a common garden to assess whether aspen leaf economics patterns follow those observed among species globally. We also evaluated leaf economics strategies in the context of insect resistance by conducting bioassays to determine the effects of plant traits on preference and performance of Lymantria dispar. We found that: 1) intraspecific trait patterns of P. tremuloides parallel those exhibited by the interspecific leaf economics spectrum, 2) herbivores preferred leaves from genotypes with resource-acquisitive strategies, and 3) herbivores also performed best on genotypes with resource-acquisitive strategies. We conclude that a leaf economics spectrum that incorporates defense traits is a useful tool for explaining intraspecific patterns of variation in plant strategies, including resistance to herbivores.