Effect of α- and β-cyclodextrins on the photochemical activity of thylakoid membranes and photosystem II particles from barley (Hordeum vulgare): oxygen evolution and whole chain electron transport

The effect of α- and β-cyclodextrin (CD) concentration (016 mM) on oxygen evolution in photosystem II (PSII) and whole chain electron transport (H2O to photosystem I (PSI)) was studied in isolated thylakoid membranes and PSII particles from barley (Hordeum vulgare L.). The CDs are cyclic oligosaccharides containing, for example, six (α-CD) or seven (β-CD) α-D-glucose residues linked by α-1,4 glycosidic bonds. These compounds alter the lipid composition of the thylakoids and most likely also the structure of their membrane proteins. We show for the first time that in the thylakoid membranes, but not in the isolated PSII particles, the relationship between oxygen evolution in PSII and the CD concentration is represented by a S-shaped (sigmoidal) curve displaying a sharp inflexion point or transition. We found, in addition, that the CDs inhibit the whole chain electron transport from H2O to methyl viologen, that is, PSI, measured as oxygen uptake, according to a nonlinear dependence that is also sigmoidal. Moreover, another interesting observation is that in the thylakoid membranes the electron transport from H2O to PSI is quite well inhibited at low CD concentrations (<46 mM), whereas the oxygen evolution in PSII is only substantially enhanced at CD concentrations greater than 810 mM. To explain this, we suggest that the mechanisms underlying the inhibition of electron transfer from H2O to PSI become operative before those giving origin to the enhancement of oxygen evolution in PSII.Key words: cyclodextrins, electron transfer, nonlinearity, oxygen evolution, photosystem, thylakoid membrane.

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Photoinhibition Causes Loss of Photochemical Activity Without Degradation of D1 Protein

Functional Plant Biology ◽

10.1071/pp9900641 ◽

1990 ◽

Vol 17 (6) ◽

pp. 641 ◽

Cited By ~ 13

Author(s):

RE Cleland ◽

RT Ramage ◽

C Critchley

Keyword(s):

Electron Transport ◽

Photosystem Ii ◽

Oxygen Evolution ◽

Charge Separation ◽

Photosynthetic Activity ◽

D1 Protein ◽

Photochemical Activity ◽

Reaction Centre ◽

Primary Charge Separation ◽

Primary Damage

Illumination of isolated thylakoids or intact leaves with excess light resulted in a decline in photosynthetic activity measured as primary charge separation in photosystem II (ΔA320), photosystem II- dependent electron transport, or leaf oxygen evolution. It is concluded that the primary damage causing photoinhibition involves inactivation of the reaction centre function, and that degradation of Dl may be a consequence of that event.

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Photosystem II Inhibition by Pyran-enamine Derivatives

Zeitschrift für Naturforschung C ◽

10.1515/znc-1993-3-409 ◽

1993 ◽

Vol 48 (3-4) ◽

pp. 163-167

Author(s):

Koichi Yoneyama ◽

Yoshihiro Nakajima ◽

Masaru Ogasawara ◽

Hitoshi Kuramochi ◽

Makoto Konnai ◽

...

Keyword(s):

Electron Transport ◽

Photosystem Ii ◽

Thylakoid Membranes ◽

Major Determinant ◽

Ps Ii ◽

Structure Activity Relationships ◽

Structure Activity

Abstract Through the studies on structure-activity relationships of 5-acyl-3-(1-aminoalkylidene)-4-hydroxy-2 H-pyran-2,6(3 H)-dione derivatives in photosystem II (PS II) inhibition, overall lipophilicity of the molecule was found to be a major determinant for the activity. In the substituted N -benzyl derivatives, not only the lipophilicity but also the electronic and steric characters of the substituents greatly affected the activity. Their mode of PS II inhibition seemed to be similar to that of DCMU , whereas pyran-enamine derivatives needed to be highly lipophilic to block the electron transport in thylakoid membranes, which in turn diminished the permeability through biomembranes.

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Investigation of the electron transfer site of p-benzoquinone in isolated photosystem II particles and thylakoid membranes using α- and β-cyclodextrins

Journal of Photochemistry and Photobiology B Biology ◽

10.1016/j.jphotobiol.2006.07.003 ◽

2006 ◽

Vol 85 (3) ◽

pp. 177-183 ◽

Cited By ~ 7

Author(s):

S. Dudekula ◽

M. Fragata

Keyword(s):

Electron Transfer ◽

Photosystem Ii ◽

Thylakoid Membranes ◽

Photosystem Ii Particles

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Inhibition of oxygen evolution by ivalin

Canadian Journal of Botany ◽

10.1139/b94-024 ◽

1994 ◽

Vol 72 (2) ◽

pp. 177-181 ◽

Cited By ~ 2

Author(s):

Ernesto Bernal-Morales ◽

Alfonso Romo De Vivar ◽

Bertha Sanchez ◽

Martha Aguilar ◽

Blas Lotina-Hennsen

Keyword(s):

Electron Transport ◽

Photosystem Ii ◽

Sesquiterpene Lactone ◽

Oxygen Evolution ◽

Electron Flow ◽

Atp Synthesis ◽

Naturally Occurring ◽

Transport Inhibitor ◽

Proton Uptake ◽

Key Words Oxygen

The inhibition of ATP synthesis, proton uptake, and electron transport (basal, phosphorylating, and uncoupled) from water to methylviologen by ivalin (a naturally occurring sesquiterpene lactone in Zaluzania triloba and Iva microcephala) indicates that it acts as electron transport inhibitor. Since photosystem I and electron transport from DPC to QA were not affected, while the electron flow of uncoupled photosystem II from H2O to DAD and from water to silicomolybdate was inhibited, we concluded that the site of inhibition of ivalin is located at the oxygen evolution level. Key words: oxygen evolution, ivalin, photosynthesis, sesquiterpene lactone.

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Comparison of Photosystem II Electron Transport and Oxygen Evolution in Two Marine Algae

Plant Production on the Threshold of a New Century ◽

10.1007/978-94-011-1158-4_54 ◽

1994 ◽

pp. 427-429

Author(s):

C. Geel ◽

J. F. H. Snel

Keyword(s):

Electron Transport ◽

Photosystem Ii ◽

Oxygen Evolution ◽

Marine Algae

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Herbicides affect fluorescence and electron transfer activity of spinach chloroplasts, thylakoid membranes and isolated Photosystem II

Bioelectrochemistry ◽

10.1016/j.bioelechem.2009.10.008 ◽

2010 ◽

Vol 79 (1) ◽

pp. 43-49 ◽

Cited By ~ 25

Author(s):

Andrea Ventrella ◽

Lucia Catucci ◽

Angela Agostiano

Keyword(s):

Electron Transfer ◽

Photosystem Ii ◽

Thylakoid Membranes ◽

Transfer Activity ◽

Spinach Chloroplasts

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The Use of O2-Evolving Subchloroplast Particles to Study Acceptor and Inhibitor Sites on the Reducing Side of Photosystem II

Zeitschrift für Naturforschung C ◽

10.1515/znc-1983-9-1020 ◽

1983 ◽

Vol 38 (9-10) ◽

pp. 793-798 ◽

Cited By ~ 4

Author(s):

W. S. Cohen ◽

J. R. Barton

Keyword(s):

Electron Transfer ◽

Electron Transport ◽

Photosystem Ii ◽

Differential Sensitivity ◽

Electron Transfer Chain ◽

Plastoquinone Pool ◽

Photosystem Ii Activity ◽

Transfer Chain ◽

Photosynthetic Electron Transfer ◽

Photosystem Ii Particles

Photosystem II particles that retain the ability to evolve O2 have been used to examine acceptor and inhibitor sites in the photosynthetic electron transfer chain between Q and plastoquinone. Employing the water to dichlorobenzoquinone reaction to assay photosystem II activity, we have demonstrated that electron transport in thylakoids and particles is equally sensitive to inhibition by DCMU. dinoseb, metribuzin, HQNO and DBMIB. Based on differential sensitivity to inhibition by DCMU vs. HQNO or DBMIB, we suggest that when synthetic quinones, e.g. 2,6-dichlorobenzoquinone operate as Hill reagents in particles they are reduced primarily by the plastoquinone pool. When synthetic quinones, e.g. 5,6-methylenedioxy-2,3-dimethyl benzoquinone act as autoxidizable acceptors they accept electron from the Q/B complex at a point that is located between the DCMU and HQNO (DBMIB) inhibition sites.

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Involvement of the Xanthophyll Cycle in Regulation of Cyclic Electron Flow around Photosystem II

Zeitschrift für Naturforschung C ◽

10.1515/znc-1996-1-209 ◽

1996 ◽

Vol 51 (1-2) ◽

pp. 47-52 ◽

Cited By ~ 2

Author(s):

W. I. Gruszecki ◽

K. Strzałk ◽

K.P. Bader ◽

A. Radunz ◽

G.H. Schmid

Keyword(s):

Electron Transport ◽

Photosystem Ii ◽

Oxygen Evolution ◽

Xanthophyll Cycle ◽

Electron Flow ◽

Photosynthetic Apparatus ◽

Photosynthetic Oxygen Evolution ◽

Cyclic Electron Transport ◽

Ps Ii ◽

Low Levels

Abstract In our previous study (Gruszecki et al., 1995) we have postulated that the mechanism of cyclic electron transport around photosystem II, active under overexcitation of the photosynthetic apparatus by light is under control of the xanthophyll cycle. The combination of different light quality and thylakoids having various levels of xanthophyll cycle pigments were applied to support this hypothesis. In the present work photosynthetic oxygen evolution from isolated tobacco chloroplasts was measured by means of mass spectrometry under conditions of high or low levels of violaxanthin, being transformed to zeaxanthin during dark incubation in an ascorbate containing buffer at pH 5.7. Analysis of oxygen evolution and of light-induced oxygen uptake indicate that the de-epoxidation of violaxanthin to zeaxanthin results in an increased cyclic electron transport around PS II, thus dimishing the vectorial electron flow from water. An effect similar to de-epoxidation was observed after incubation of thylakoid membranes with specific antibodies against violaxanthin.

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