Embryological features of taxonomic significance in the genus Nyssa
The embryological investigation of Nyssa aquatica L., N. biflora Walt., and N. sylvatica Marsh. reported here clearly delimits similarities and variations among the three species. Such evidence supports the retention of Nyssaceae as a family apart from Cornaceae. The major distinctions between the two families occur in the differentiation of the megasporocyte, development of the nucellus, degeneration of defunct megaspores, and growth pattern of the megagametophyte. Major points of similarity between the two families are the occurrence of unitegmic, pendulous, anatropous ovules, a single hypodermal archesporial cell, unequal dyad cells, hooked synergids, polar nuclei fusing before syngamy, and cellular endosperm. These same features serve also to show that Nyssaceae belongs in the order Cornales rather than Myrtales as suggested by several workers.Strong evidence is provided for the recognition of N. biflora as a distinct species rather than a variety of N. sylvatica as previously proposed by several workers. Points of distinction between the two taxa are found in the shape of the ovule, growth pattern of the nucellar apex and integument, position of the nuclei in the four-nucleate megagametophyte, and the growth pattern of the megagametophyte.Embryological features further indicate a closer relationship between N. aquatica and N. biflora than between either one and N. sylvatica. The features of greatest similarity between N. aquatica and N. biflora are the shape of the ovule and megasporocyte, growth pattern of the megagametophyte, presence of hooked synergids, and the occasional occurrence of bisporic megagametogenesis.