Growth of filamentous marine fungi in a continuous culture system

1976 ◽  
Vol 54 (9) ◽  
pp. 893-899 ◽  
Author(s):  
L. M. Churchland ◽  
M. McClaren

A continuous culture system has been designed for the cultivation of certain species of marine fungi. Growth in this apparatus was compared with growth in batch culture. Growth of the three marine species tested (Zalerion maritimum, Humicola alopallonella, and Monodictys pelagica) was significantly higher in continuous culture than in batch culture.Growth of two isolates of Z. maritimum was compared on three inorganic nitrogen sources and one organic nitrogen source. Growth of the fungus in the continuous system differed both qualitatively and quantitatively from that in batch culture. Both isolates showed greater growth on the organic nitrogen source in the closed system. In the continuous system, growth was equivalent on all nitrogen sources.

2015 ◽  
Vol 77 (31) ◽  
Author(s):  
Huszalina Hussin ◽  
Madihah Md Salleh ◽  
Chong Chun Siong ◽  
Muhammad Abu Naser ◽  
Suraini Abd- Aziz ◽  
...  

The recent study has demonstrated the effects of different nitrogen sources on vanillin production by Phanerochaete chrysosporium. Primary screening supported maximum biotransformation of ferulic acid (from lemongrass leaves hydrolysate) to vanillin by using ammonium chloride and yeast extract as inorganic and organic nitrogen source, respectively. With the 2-level factorial analysis, the optimum conditions of vanillin production from ferulic acid by P. chrysosporium was achieved at 0.192g/L with a molar yield of 24.5%.


Chemosphere ◽  
1998 ◽  
Vol 37 (2) ◽  
pp. 257-264 ◽  
Author(s):  
Jianlong Wang ◽  
Ping Liu ◽  
Hanchang Shi ◽  
Yi Qian

2014 ◽  
Vol 96 ◽  
pp. 111-118 ◽  
Author(s):  
David Feria-Gervasio ◽  
William Tottey ◽  
Nadia Gaci ◽  
Monique Alric ◽  
Jean-Michel Cardot ◽  
...  

1989 ◽  
Vol 61 (5) ◽  
pp. 463-473
Author(s):  
Harri Miettinen ◽  
Jouko Setälä

The present report describes an in vitro continuous culture system to simulate rumen fermentation. The complete assembly consists of six culture vessels (liquid volume 700 ml) fed twice daily with finely ground feed. The artificial saliva enters the vessel continuously, and the effluent leaves it continuously through the overflow port. The intermittent stirring of the fermentor content and the pH regulation are automatically controlled by a desktop computer. Two replicate experiments with ten fermentors given a diet of silage (50 %) and barley (50 %) were made in order to evaluate the system. The results indicate that the system reaches steady-state conditions within three to five days, ammonia concentration being an exeption. It takes for the ammonia concentration approximately 11—14 days to stabilize. The plateau values for the total volatile fatty acid (VFA) concentrations, the molar proportions of individual VFAs, and the ammonia concentrations were found to be within the accepted range in the rumen of animals given similar diets or in other artificial rumen systems. There was a tenfold decrease in the numbers of protozoa in the fermentors during the first four days of incubation. However, the average plateau value for the protozoa numbers (2.5 x 104/ml) is in the same range as in the dual flow continuous culture systems. The efficiency of the microbial N production was higher than that usually observed in vivo or in vitro (45 vs. 30 g/kg organic matter digested). The results indicate that this continuous culture system provides a reasonable estimate of rumen fermentation.


2017 ◽  
Vol 95 (suppl_4) ◽  
pp. 145-145
Author(s):  
C. B. Sampaio ◽  
E. Marostegan de Paula ◽  
L. Galoro da Silva ◽  
V. Brandao ◽  
X. Dai ◽  
...  

2002 ◽  
Vol 5 (3) ◽  
pp. 329-331 ◽  
Author(s):  
Che Husna Azhari ◽  
Aulia Saad ◽  
Wan Mohtar Wan Yusoff ◽  
A. Ikram .

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