Isolation of a dwarf plant responsive to exogenous GA3 from anther cultures of birdsfoot trefoil

1981 ◽  
Vol 59 (7) ◽  
pp. 1338-1342 ◽  
Author(s):  
Dwight T. Tomes ◽  
R. L. Peterson
Keyword(s):  
Chromosome Number ◽  
Culture Medium ◽  
Shoot Tip ◽  
Birdsfoot Trefoil ◽  
Shoot Tip Culture ◽  
Dwarf Plant ◽  
Internode Growth ◽  
Anther Cultures ◽  
Normal Genotype

A dwarf plant isolated from anther culture of a normal genotype of birdsfoot trefoil (Lotus corniculatus L. cv. Leo) has been isolated, characterized, and maintained as an in vitro shoot-tip culture. The dwarf condition is the result of a much shorter internode length when compared with a normal genotype in shoot tip culture. Dwarf shoots which are typically rosette respond to increased GA3 levels by increased internode growth. Normal genotypes are inhibited and eventually die as a result of increased levels of GA3 in the culture medium. This dwarf has the normal tetraploid chromosome number of 24, and although stable in shoot tip cultures since 1977, has not survived outside of in vitro shoot tip cultures.

scholarly journals In vitro propagation and cytological analysis of Sophora mollis Royle: an endangered medicinal shrub

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Aakriti Bhandari ◽  
Harminder Singh ◽  
Amber Srivastava ◽  
Puneet Kumar ◽  
G. S. Panwar ◽  
...  
Keyword(s):  
Chromosome Number ◽  
Average Length ◽  
Germplasm Conservation ◽  
Basic Chromosome Number ◽  
Shoot Tip ◽  
Ex Situ ◽  
Ms Medium ◽  
Basic Chromosome ◽  
Shoot Tip Explants

Abstract Background Sophora mollis Royle (family Fabaceae, subfamily-Papilionaceae) is a multipurpose legume distributed in plains and foothills of the North-West Himalaya to Nepal and is facing high risk of extinction due to habitat loss and exploitation by the local people for its fuel and fodder values. Therefore, the present study was conducted to standardize a micropropagation protocol for Sophora mollis by using shoot tip explants and to study the meiotic chromosome count in the species. Results Multiple shoots were induced in shoot tip explants of Sophora mollis in Murashige and Skoog medium supplemented with different concentrations of cytokinins alone (BAP, TDZ, and Kinetin) and in combination with varying concentrations of NAA. MS medium supplemented with BAP (8.9 μM) was observed to be the optimal medium for multiple shoot induction and maximum 25.32 shoots per explant was obtained with average length of 4.5 ± 0.8 cm. In vitro developed shoots were transferred onto rooting media supplemented with different concentrations of auxin (IAA, IBA, and NAA). Maximum 86% rooting was observed in half-strength MS medium supplemented with 21.20 μM NAA with an average of 21.26 roots per culture. In vitro raised plantlets were adapted to greenhouse for better acclimatization and 60% plants were successfully transferred to the open environment. Based on the chromosome counts available from the literature and the current study, the species tend to show a basic chromosome number of x = 9. Conclusion The micropropagation protocol standardized can be helpful for the ex situ mass multiplication and germplasm conservation of the endangered species. Moreover, the ex situ conservation approach will be helpful in actively bridging the gap between ex situ and in situ approaches through the reintroduction of species in the wild. The cytological studies revealed the basic chromosome number x = 9 of the species.

scholarly journals Cleaning cassava genotypes infected with cassava frogskin disease via in vitro shoot tip culture

2017 ◽  
Vol 16 (2) ◽  
Author(s):  
M.J.S. Carvalho ◽  
E.J. Oliveira ◽  
A.S. Souza ◽  
J.S. Pereira ◽  
M.S.A.S. Diamantino ◽  
...  
Keyword(s):  
Shoot Tip ◽  
Shoot Tip Culture

In vitro Clonal Propagation of Cucumis sativus L. By Shoot Tip Culture

2007 ◽  
Vol 7 (4) ◽  
pp. 653-657 ◽  
Author(s):  
Jafar Mohammadi ◽  
Nuray Sivritepe .
Keyword(s):  
Cucumis Sativus ◽  
Clonal Propagation ◽  
Shoot Tip ◽  
Shoot Tip Culture ◽  
Cucumis Sativus L ◽  
In Vitro Clonal Propagation

scholarly journals Protocol optimization for in vitro propagation of Kulfo, orange flesh sweet potato (Ipomoea batatas) variety using shoot tip culture

2020 ◽  
Vol 14 (10) ◽  
pp. 395-401
Author(s):  
Beyene Belachew ◽  
Menamo Temesgen ◽  
Haile Gizachew
Keyword(s):  
Sweet Potato ◽  
In Vitro Propagation ◽  
Ipomoea Batatas ◽  
Shoot Tip ◽  
Shoot Tip Culture ◽  
Protocol Optimization

scholarly journals In vitro Mass Propagation of a Ground Orchid - Phaius tancarvilleae (L'Her.) Blume through Shoot Tip Culture

2011 ◽  
Vol 21 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Bijaya Pant ◽  
Sumitra Shrestha
Keyword(s):  
High Frequency ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Shoot Tip ◽  
Mass Propagation ◽  
Shoot Tip Culture ◽  
Shoot Tip Explants ◽  
Direct Shoot

High frequency direct shoot proliferation was induced from the shoot tip explants derived from the in vitro grown seedlings of a critically endangered and horticulturally important ground orchid Phaius tancarvilleae (L'Her) Blume. Shoot tip explants cultured on solidified MS with alone or combination of various concentrations of NAA and BAP produced shoots and multiple shoots. The maximum number of healthy shoots was observed on MS with BAP (1.0 mg/l) with an average of 13.3 shoots per culture in 20 weeks; where shoot multiplication was initiated after 4 weeks of culture. Regenerated shoots rooted on MS with various concentrations of NAA, IAA, IBA. MS with NAA (0.5 mg/l) was the most appropriate condition for rooting. The well developed in vitro rooted plantlets were hardened successfully in the potting mixture containing cocopeat and sphagnum moss in the ratio of 2 : 1.   Key words: Mass propagation, Phaius tancarvilleae, shoot multiplication   D. O. I. 10.3329/ptcb.v21i2.10241   Plant Tissue Cult. & Biotech. 21(2): 181-188, 2011 (December)

THE INFLUENCE OF HIGH TEMPERATURES AND BENZYLADENINE ON ROOT INDUCTION DURING IN VITRO SHOOT TIP CULTURE IN VITIS VINIFERA L.

1988 ◽  
pp. 479-481 ◽  
Author(s):  
G. Fanizza ◽  
L. Ricciardi ◽  
D. Boscia ◽  
O. Silvestroni
Keyword(s):  
Vitis Vinifera ◽  
High Temperatures ◽  
Shoot Tip ◽  
Vitis Vinifera L ◽  
Root Induction ◽  
Shoot Tip Culture

scholarly journals An Efficient Method for In Vitro Shoot-Tip Culture and Sporophyte Production Using Selaginella martensii Spring Sporophyte

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 235 ◽  
Author(s):  
Kyungtae Park ◽  
Bo Kook Jang ◽  
Ha Min Lee ◽  
Ju Sung Cho ◽  
Cheol Hee Lee
Keyword(s):  
Nitrogen Sources ◽  
Culture Conditions ◽  
Shoot Tips ◽  
Shoot Tip ◽  
Soil Conditions ◽  
Ms Medium ◽  
Ex Vitro ◽  
Shoot Tip Culture ◽  
Propagation Methods

Selaginella martensii, an evergreen perennial fern that is native to South America and New Zealand, is named “frosty fern” because of its beautiful white-colored leaves and it is used as an ornamental plant. Efficient propagation methods for this species have not been developed. We aimed to develop an efficient propagation method for S. martensii through in vitro culture. We investigated culture conditions that are suitable for shoot-tip proliferation and growth. The optimum shoot-tip culture conditions were determined while using Murashige and Skoog (MS) medium (quarter, half, full, or double strength) and macronutrients (sucrose and two nitrogen sources) at various concentrations. In MS medium, the shoot tips formed a maximum of 6.77 nodes per explant, and each node formed two new shoot tips (i.e., 26 or 64 shoot tips). When using branching segments containing an angle meristem, the shoot-to-rhizophore formation ratio could be controlled by medium supplementation with plant-growth regulators. Sporophytes that were grown from shoot tips in vitro were acclimated in ex vitro soil conditions and successfully survived in the greenhouse. Numerous shoot tips could be obtained from in vitro-grown sporophytes and be proliferated ex vitro to produce a large number of plants. This method provides a way of shortening the time that is required for producing a large stock of S. martensii planting material.

Sign in / Sign up

Export Citation Format

Share Document