Effects of time of nitrate application on nitrate reductase activity, nitrate uptake, and symbiotic dinitrogen fixation in faba bean and pea

1988 ◽  
Vol 66 (8) ◽  
pp. 1646-1652 ◽  
Author(s):  
François-P. Chalifour ◽  
Louise M. Nelson
Keyword(s):  
Nitrate Reductase ◽  
Faba Bean ◽  
Nitrate Reductase Activity ◽  
Nitrate Uptake ◽  
Rhizobium Leguminosarum ◽  
Reductase Activity ◽  
Pisum Sativum L ◽  
Vicia Faba L ◽  
Total Plant ◽  
Lower Capacity

The effects of increasing concentrations of [Formula: see text] (0, 5, 10, or 15 mol∙m−3) supplied at planting or 7 or 14 days after planting were studied in faba bean (Vicia faba L.) and pea (Pisum sativum L.) inoculated with Rhizobium leguminosarum biovar viceae isolate 175F19. Averaged over all times of [Formula: see text] application, N2 fixation (acetylene reduction) was inhibited significantly more in pea than in faba bean. Times of [Formula: see text] application had little effect on N2 fixation for faba bean. In contrast, N2 fixation in pea was most strongly inhibited when [Formula: see text] was applied at planting and showed a decreasing sensitivity as [Formula: see text] applications were delayed to 7 and 14 days after planting. At all times of [Formula: see text] application, as the levels of [Formula: see text] increased, the proportion of total plant nitrate reductase activity contributed by leaves and roots remained relatively constant in faba bean, but these proportions increased and decreased, respectively, in pea. Nitrate was taken up at higher levels and at a greater rate in pea than in faba bean. The lower capacity for [Formula: see text] uptake in faba bean could contribute to the greater tolerance of the symbiosis to [Formula: see text] compared with that in pea.

Short-term effects of nitrate on nitrate reductase activity and symbiotic dinitrogen fixation in faba bean and pea

1988 ◽  
Vol 66 (8) ◽  
pp. 1639-1645 ◽  
Author(s):  
Francois-P. Chalifour ◽  
Louise M. Nelson
Keyword(s):  
Nitrate Reductase ◽  
Faba Bean ◽  
Nitrite Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Inhibitory Effects ◽  
Short Term ◽  
Pisum Sativum L ◽  
Vicia Faba L ◽  
Legume Symbiosis

The effects of a short-term supply of combined nitrogen (N) on the Rhizobium–legume symbiosis were studied in faba bean (Vicia faba L.) and pea (Pisum sativum L.) nodulated by R. leguminosarum biovar viceae isolate 175F5 or 175F19 and subjected to increasing levels of [Formula: see text] (0, 5, 10, or 20 mol∙m−3) from 28 to 36 days after planting. Trends in N2-fixing activity (acetylene reduction) showed that faba bean was more tolerant to [Formula: see text] than pea with isolate 175F5 but not with isolate 175F19. Nitrite reductase activities in the leaf, root, and nodule cytosol fractions were severalfold higher than nitrate reductase activities for both hosts. The levels of these enzymes in the nodule cytosol, the absence of bacteroid nitrate reductase and the lack of induction of bacteroid nitrite reductase in response to [Formula: see text] addition are consistent with the lack of [Formula: see text] accumulation in nodules of both hosts. Therefore, it is unlikely that the inhibitory effects of [Formula: see text] on N2 fixation are due to [Formula: see text] inhibition of nitrogenase. The relative levels of nitrate reductase activity in the root and nodule cytosol fractions were, respectively, higher and lower in the two faba bean symbioses ([Formula: see text] tolerant) and in the pea–175F5 symbiosis ([Formula: see text] sensitive) than in the pea–175F19 symbiosis ([Formula: see text] tolerant).

Nitrate Uptake and the Induction of Nitrate Reductase Activity in Wheat (Triticum aestivumL.) Seedlings

1981 ◽  
Vol 32 (1) ◽  
pp. 9-18 ◽  
Author(s):  
P. W. JONES ◽  
C. B. JOHNSON ◽  
W. J. WHITTINGTON
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Nitrate Uptake ◽  
Reductase Activity

Effects of continuous combined nitrogen supply on symbiotic dinitrogen fixation of faba bean and pea inoculated with different rhizobial isolates

1987 ◽  
Vol 65 (12) ◽  
pp. 2542-2548 ◽  
Author(s):  
François-P. Chalifour ◽  
Louise M. Nelson
Keyword(s):  
Faba Bean ◽  
Acetylene Reduction ◽  
Rhizobium Leguminosarum ◽  
Combined Nitrogen ◽  
Pisum Sativum L ◽  
Legume Symbiosis ◽  
Total Plant ◽  
Continuous Presence ◽  
Symbiotic Partner ◽  
Rhizobial Isolates

Combined nitrogen (N) has adverse effects on virtually all stages of the Rhizobium–legume symbiosis. Tolerance to combined N varies among legume hosts and rhizobial isolates, but the contribution of each symbiotic partner is not well established. The effects of combined N were studied in faba bean (Vicia faba L.) and pea (Pisum sativum L.), using the same Rhizobium leguminosarum isolates for both hosts. In one experiment, faba bean and pea were inoculated individually with four rhizobial isolates and grown for 28 days in the continuous presence of 0, 2.5, 5.0, or7.5 mol m−3 NH4NO3. For each isolate, faba bean was consistently more tolerant to combined N than pea as shown by significantly smaller rates of decrease in N2-fixing activity (acetylene reduction) in faba bean than in pea. The results were substantiated by those of a similar experiment in which increasing levels of 15N-labeled [Formula: see text] (5, 10, or 15 mol m−3) were supplied continuously to faba bean and pea inoculated individually with two rhizobial isolates. Comparisons of the different symbioses based on the proportion of total plant N derived from N2 fixation confirmed the conclusions reached using acetylene reduction activities.

The relation between nitrate reductase activity and grain nitrogen productivity in wheat

1975 ◽  
Vol 26 (1) ◽  
pp. 1 ◽  
Author(s):  
MJ Dalling ◽  
GM Halloran ◽  
JH Wilson
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Selection Criterion ◽  
Activity Levels ◽  
Plant Nitrogen ◽  
Nitrogen Productivity ◽  
Nitrogen Redistribution ◽  
Total Plant ◽  
Grain Nitrogen

The seasonal patterns of leaf nitrate reductase activity were compared in five wheat cultivars which differ widely in their capacities to accumulate grain nitrogen. Significant differences in the average levels of nitrate reductase activity were observed between cultivars. Total seasonal nitrate reductase activity was closely related to total plant nitrogen at maturity. Grain nitrogen was only related to total seasonal nitrate reductase activity when allowance was made for significant differences between cultivars in nitrogen redistribution patterns. The significance of these results with respect to the possible use of nitrate reductase activity levels as a selection criterion for nitrogen productivity is discussed.

scholarly journals Effects of Nitrite, Chlorate, and Chlorite on Nitrate Uptake and Nitrate Reductase Activity

1992 ◽  
Vol 100 (2) ◽  
pp. 644-650 ◽  
Author(s):  
M. Yaeesh Siddiqi ◽  
Bryan J. King ◽  
Anthony D. M. Glass
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Nitrate Uptake ◽  
Reductase Activity

scholarly journals Induction of nitrate uptake and nitrate reductase activity in trembling aspen and lodgepole pine

1998 ◽  
Vol 21 (10) ◽  
pp. 1039-1046 ◽  
Author(s):  
X. Min ◽  
M. Y. Siddiqi ◽  
R. D. Guy ◽  
A. D. M. Glass ◽  
H. J. Kronzucker
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Nitrate Uptake ◽  
Lodgepole Pine ◽  
Reductase Activity ◽  
Trembling Aspen

scholarly journals Physiological response of a golden tide alga (<i>Sargassum muticum</i>) to the interaction of ocean acidification and phosphorus enrichment

2017 ◽  
Vol 14 (3) ◽  
pp. 671-681 ◽  
Author(s):  
Zhiguang Xu ◽  
Guang Gao ◽  
Juntian Xu ◽  
Hongyan Wu
Keyword(s):  
Nitrate Reductase ◽  
Ocean Acidification ◽  
Soluble Protein ◽  
Nitrate Reductase Activity ◽  
Nitrate Uptake ◽  
Dark Respiration ◽  
Reductase Activity ◽  
Soluble Carbohydrates ◽  
Promoting Effect ◽  
Nitrate Uptake Rate

Abstract. The development of golden tides is potentially influenced by global change factors, such as ocean acidification and eutrophication, but related studies are very scarce. In this study, we cultured a golden tide alga, Sargasssum muticum, at two levels of pCO2 (400 and 1000 µatm) and phosphate (0.5 and 40 µM) to investigate the interactive effects of elevated pCO2 and phosphate on the physiological properties of the thalli. Higher pCO2 and phosphate (P) levels alone increased the relative growth rate by 41 and 48 %, the net photosynthetic rate by 46 and 55 %, and the soluble carbohydrates by 33 and 62 %, respectively, while the combination of these two levels did not promote growth or soluble carbohydrates further. The higher levels of pCO2 and P alone also enhanced the nitrate uptake rate by 68 and 36 %, the nitrate reductase activity (NRA) by 89 and 39 %, and the soluble protein by 19 and 15 %, respectively. The nitrate uptake rate and soluble protein was further enhanced, although the nitrate reductase activity was reduced when the higher levels of pCO2 and P worked together. The higher pCO2 and higher P levels alone did not affect the dark respiration rate of the thalli, but together they increased it by 32 % compared to the condition of lower pCO2 and lower P. The neutral effect of the higher levels of pCO2 and higher P on growth and soluble carbohydrates, combined with the promoting effect on soluble protein and dark respiration, suggests that more energy was drawn from carbon assimilation to nitrogen assimilation under conditions of higher pCO2 and higher P; this is most likely to act against the higher pCO2 that caused acid–base perturbation via synthesizing H+ transport-related protein. Our results indicate that ocean acidification and eutrophication may not boost golden tide events synergistically, although each one has a promoting effect.

Sign in / Sign up

Export Citation Format

Share Document