Inter-Species Relationships Within the Genus Oncorhynchus Based on Biochemical Systematics

1966 ◽  
Vol 23 (1) ◽  
pp. 101-107 ◽  
Author(s):  
H. Tsuyuki ◽  
E. Roberts
Keyword(s):  
Phylogenetic Relationships ◽  
Gel Electrophoresis ◽  
Plasma Proteins ◽  
Disc Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Species Relationships ◽  
Oncorhynchus Masou ◽  
Starch Gel ◽  
Specific Muscle ◽  
Species Specific

The species specific muscle myogens of Salmo gairdnerii, Oncorhynchus masou, O. masou ishikawae, O. kisutch, O. tshawytscha, O. keta, O. nerka, and O. gorbuscha are compared by starch gel electrophoresis. Plasma proteins of these same species are also examined by polyacrylamide disc electrophoresis. The range of usefulness of muscle myogens in species identification, and equally significantly, their value in establishing phylogenetic relationships of closely related groups, as the genus Oncorhynchus, are discussed. The myogen patterns of O. keta and O. gorbuscha from the Asiatic and North American coasts were found to be identical, further supporting the concept of absolute species specificity of these patterns.

Comparative Zone Electropherograms of Muscle Myogens and Blood Proteins of Adult and Ammocoete Lamprey

1967 ◽  
Vol 24 (6) ◽  
pp. 1269-1273 ◽  
Author(s):  
J. F. Uthe ◽  
H. Tsuyuki
Keyword(s):  
Great Lakes ◽  
Gel Electrophoresis ◽  
Plasma Proteins ◽  
Disc Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Blood Proteins ◽  
Adult Form ◽  
Starch Gel

Polyacrylamide disc electrophoresis of plasma proteins and starch-gel electrophoresis of hemoglobins and muscle myogens of adult and ammocoete forms of three species of Great Lakes lamprey were carried out. Blood proteins were shown to undergo marked changes upon transformation of the ammocoete into the adult form. Muscle myogen did not undergo any change during transformation. The muscle myogen electropherograms of Ichthyomyzon unicuspis and Lampetra lamottei were found to be the same.

CONFIRMATION OF PLASMA PROTEIN CHANGES IN AVIAN ERYTHROBLASTOSIS

1964 ◽  
Vol 42 (2) ◽  
pp. 293-297 ◽  
Author(s):  
M. Merriman ◽  
C. le Q. Darcel
Keyword(s):  
Plasma Protein ◽  
Gel Electrophoresis ◽  
Plasma Proteins ◽  
Starch Gel Electrophoresis ◽  
Starch Gel

Alterations of the plasma proteins have been previously demonstrated in avian erythroblastosis by paper and now by starch gel electrophoresis. With the latter technique, eight protein zones are recognized in normal plasmas. Heparin contributes an additional non-staining zone. In leukemic plasmas two more zones occur while another zone shows significant retardation.Heparin is not responsible for these changes because they are also observed in oxalated plasmas, but there is evidence of increased binding of heparin in leukemic plasma.

scholarly journals Genetics of plasma transferrins in the mouse

1960 ◽  
Vol 1 (3) ◽  
pp. 431-438 ◽  
Author(s):  
B. L. Cohen
Keyword(s):  
Gel Electrophoresis ◽  
Molecular Basis ◽  
Plasma Proteins ◽  
Inbred Strains ◽  
The Other ◽  
Starch Gel Electrophoresis ◽  
Widespread Distribution ◽  
Agouti Locus ◽  
Inbred Strains Of Mice ◽  
Starch Gel

1. The plasma proteins of six inbred strains of mice have been studied, using starch-gel electrophoresis.2. The existence of two alternative plasma transferrin (β-globulin) phenotypes has been demonstrated. Five of the strains have one of these and one strain has the other. Each of the two transferrin patterns comprises three (or possibly only two) electrophoretic bands. The two patterns differ in all of these bands.3. The two transferrin types recognized are determined by a pair of allelic, autosomal genes (designated TrfA and TrfB). The TrfA phenotype (CBA strain) is determined by the genotype TrfA/TrfA, and the TrfB phenotype (A, C57BL, JU, KL, RIII strains) by the genotype TrfB/TrfB. The phenotype TrfAB, of the heterozygote (genotype TrfA/TrfB), is distinguishable and shows four (or possibly only three) bands. In this way it closely resembles a mixture of equal parts of TrfA and TrfB plasma.4. No linkage was detected between the Trf locus and sex, the agouti locus or the haemoglobin locus.5. The possible molecular basis of the action of the transferrin alleles in the mouse, and the widespread distribution in mammals of polymorphism involving the transferrins, are discussed.

FURTHER STUDIES OF CHANGES IN PLASMA PROTEINS IN AVIAN ERYTHROBLASTOSIS: I. TWO-DIMENSIONAL STARCH GEL ELECTROPHORESIS

1965 ◽  
Vol 43 (10) ◽  
pp. 1661-1665 ◽  
Author(s):  
Mohendra Merriman
Keyword(s):  
Plasma Protein ◽  
Gel Electrophoresis ◽  
Plasma Proteins ◽  
Starch Gel Electrophoresis ◽  
Two Dimensional ◽  
Starch Gel

Plasma protein changes in avian erythroblastosis previously studied with paper and starch gel electrophoresis have now been examined with a two-dimensional technique combining the two methods. The differences affect chiefly one zone which migrates in the α-globulin region.

scholarly journals LINKAGE ANALYSIS OF THE MULTILOCUS GLUCOSEPHOSPHATE ISOMERASE ISOZYME SYSTEM IN SUNFISH (CENTRARCHIDAE, TELEOSTII)

Genetics ◽  
1976 ◽  
Vol 82 (1) ◽  
pp. 35-42
Author(s):  
Gregory S Whitt ◽  
William F Childers ◽  
James B Shaklee ◽  
Janet Matsumoto
Keyword(s):  
High Frequency ◽  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Glucosephosphate Isomerase ◽  
Electrophoretic Mobilities ◽  
Starch Gel ◽  
Allelic Isozymes ◽  
F2 Generation ◽  
Species Specific ◽  
Green Sunfish

ABSTRACT The purpose of the present investigation is to determine whether the two duplicated glucosephosphate isomerase (EC 5.3.1.9) loci Gpi-A and Gpi-B reside on the same chromosome in teleostean fishes. Interspecific sunfish hybrids were employed for the cross because of the different species-specific electrophoretic mobilities of the allelic isozymes at each GPI locus and because of their genomic compatibility. F1 sunfish hybrids, formed from a male warmouth (Lepomis gulosus) × female green sunfish (L. cyanellus) cross, were mated to form the F2 generation. The number of each of the nine different isozyme phenotypes, revealed by starch gel electrophoresis, was determined using 256 F2 individuals. The high frequency of recombinant phenotypes in the F2 generation indicated that the two GPI loci are not linked. An excess of F2 individuals heterozygous at both loci was observed and is interpreted as being caused by heterosis. The absence of linkage for the homologous loci encoding GPI subunits and for other multilocus isozyme systems is consistent with the postulate that the genomes of present-day vertebrates arose through one or more polyploidization events early in vertebrate evolution.

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