Mitochondrial DNA Variation among Lake Trout (Salvelinus namaycush) Strains Stocked into Lake Ontario

1993 ◽  
Vol 50 (11) ◽  
pp. 2397-2403 ◽  
Author(s):  
Peter M. Grewe ◽  
Charles C. Krueger ◽  
Charles F. Aquadro ◽  
Eldredge Bermingham ◽  
Harold L. Kincaid ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Lake Trout ◽  
Lake Ontario ◽  
Restriction Enzymes ◽  
Salvelinus Namaycush ◽  
Purification Method ◽  
Dna Variation ◽  
Total Dna ◽  
Mixed Stock ◽  
Haplotype Information

Mitochondrial DNA (mtDNA) variation was examined in 492 fish representing six lake trout (Salvelinus namaycush) strains used for stocking and restoring populations in Lake Ontario. mtDNA was extracted from 432 fish by a total DNA isolation protocol (CTAB). mtDNA was also extracted from 60 additional fish using the purification method of CsCl ultracentrifugation. The more rapid CTAB protocol made feasible analysis of sample sizes (n ≥ 80 per strain) required as baseline data for future mixed-stock analysis (MSA). Restriction enzymes AvaI, BamHI, HinfI, and TaqI resolved seven mtDNA haplotypes and were used to characterize fish from each of six strains (Clearwater, Jenny, Killala, Manitou, Seneca, and Superior). Frequencies of these haplotypes were significantly different among the six strains (p < 0.001). Differences between haplotype frequencies of the Killala and Superior strains were striking and permit greater discrimination of these strains than allozyme data. The level of differentiation observed among strains indicates that mtDNA haplotype information will enhance the ability of MSA to determine the hatchery strains that serve as parents to lake trout fry collected from Lake Ontario.

Mitochondrial DNA Diversity among Broodstocks of the Lake Trout, Salvelinus namaycush

1988 ◽  
Vol 45 (12) ◽  
pp. 2114-2122 ◽  
Author(s):  
Peter M. Grewe ◽  
Paul D. N. Hebert
Keyword(s):  
Mitochondrial Dna ◽  
Great Lakes ◽  
Lake Trout ◽  
Natural Populations ◽  
Restriction Enzymes ◽  
Salvelinus Namaycush ◽  
Brood Stock ◽  
Base Pairs ◽  
Specific Distribution ◽  
Western Great Lakes

Mitochondrial DNA was purified from 151 lake trout (Salvelinus namaycush) representing nine brood stocks along with an additional 30 fish from two natural populations. Eighteen restriction endonucleases were employed to analyze 126 brood stock fish. Two of these enzymes (Hind III and Bam HI) were used to examine the fish from the natural populations (Hare Island (Lake Superior) and North Knife Lake (Manitoba)) and the remaining brood stock samples from the Manitou (12 fish) and Seneca (13 fish) strains. The mitochondrial genome of the lake trout was 16 800 ± 200 base pairs in length. A single heteroplasmic individual was discovered in the Manitou strain. It contained two genomes, the less frequent of which was 20 base pairs shorter than the other. The 18 restriction enzymes resolved 13 mitochondrial clones which fell into three major groups. These clonal groups, which can be identified by their Bam HI restriction phenotypes, have a specific distribution: a western Great Lakes group, a central Great Lakes group, and an eastern Great Lakes group. Seven mitochondrial clones were unique to a particular stock. In addition there were dramatic shifts in the relative proportions of the six remaining mitochondrial clones among the brood stocks. These results indicate that mitochondrial DNA markers have great potential for the identification and management of lake trout strains.

Evidence of successful river spawning by lake trout (Salvelinus namaycush) in the Lower Niagara River, Lake Ontario

2021 ◽  
Author(s):  
Alexander Gatch ◽  
Dimitry Gorsky ◽  
Zy Biesinger ◽  
Eric Bruestle ◽  
Kelley Lee ◽  
...  
Keyword(s):  
Lake Trout ◽  
Lake Ontario ◽  
Salvelinus Namaycush ◽  
Niagara River

Mitochondrial and nuclear DNA variation, genotype fingerprinting and genetic relationships in lentil (Lens culinaris Medik.)

1997 ◽  
Vol 77 (4) ◽  
pp. 515-521 ◽  
Author(s):  
Om P. Rajora ◽  
John D. Mahon
Keyword(s):  
Mitochondrial Dna ◽  
Restriction Fragment ◽  
Lens Culinaris ◽  
Nuclear Dna ◽  
Genetic Relationships ◽  
Restriction Enzymes ◽  
Dna Variation ◽  
Apocytochrome B ◽  
Nuclear Dna Variation ◽  
Mean Similarity

Mitochondrial DNA (mtDNA) and nuclear DNA (nuDNA) variations were examined in six cultivars of Lens culinaris ssp. culinaris and two (mtDNA) or one (nuDNA) accession(s) of L. culinaris ssp. orientalis. Total leaf DNA was digested with up to 15 restriction endonucleases, separated by agarose gel electrophoresis and trasferred to nylon membranes. To examine mtDNA variation, blots were probed with mtDNA coding for cytochrome c oxidase I (coxI) and ATPase 6 (atp6) of both wheat and maize as well as apocytochrome b (cob) and Orf25 (orf25) of wheat. Sixteen combinations of mtDNA probes and restriction enzymes revealed 34 fragments that discriminated between at least two lentil accessions. For nuDNA analysis, probes from cDNA and genomic DNA clones of lentil were used to probe the same blots, and identified 46 diagnostic fragments from 19 probe/enzyme combinations. Each lentil accession could be unequivocably distinguished from all others on the basis of both mitochondrial and nuclear DNA fragment patterns. The mitochondrial restriction fragment similarities ranged from 0.944 to 0.989, with a mean of 0.970 but nuclear restriction fragment similarities varied from 0.582 to 0.987, with a mean of 0.743. The apparent genetic relationships among accessions differed according to the source of DNA examined, although the commercial varieties Laird, Brewer and Redchief showed similarly high levels of mean similarity with both nuclear (0.982) and mitochondrial DNA (0.983). Key words: Lens culinaris Medik., genetic variation, mitochondrial, nuclear, DNA, lentil

Sexual difference in PCB concentrations of lake trout (Salvelinus namaycush) from Lake Ontario

2010 ◽  
Vol 408 (7) ◽  
pp. 1725-1730 ◽  
Author(s):  
Charles P. Madenjian ◽  
Michael J. Keir ◽  
D. Michael Whittle ◽  
George E. Noguchi
Keyword(s):  
Sexual Difference ◽  
Lake Trout ◽  
Lake Ontario ◽  
Salvelinus Namaycush

Dechlorane plus monoadducts in a lake ontario (Canada) food web and biotransformation by lake trout (Salvelinus namaycush)liver microsomes

2013 ◽  
Vol 32 (6) ◽  
pp. 1376-1381 ◽  
Author(s):  
Gregg T. Tomy ◽  
Ed Sverko ◽  
Vince Palace ◽  
Bruno Rosenberg ◽  
Robert McCrindle ◽  
...  
Keyword(s):  
Food Web ◽  
Lake Trout ◽  
Liver Microsomes ◽  
Lake Ontario ◽  
Salvelinus Namaycush ◽  
Dechlorane Plus

Mitochondrial DNA Variation and Mixed-Stock Analysis of Recreational and Commercial Walleye Fisheries in Eastern Lake Erie

2003 ◽  
Vol 23 (2) ◽  
pp. 431-440 ◽  
Author(s):  
Michael H. Gatt ◽  
Tara L. McParland ◽  
Larry C. Halyk ◽  
Moira M. Ferguson
Keyword(s):  
Mitochondrial Dna ◽  
Lake Erie ◽  
Dna Variation ◽  
Mixed Stock Analysis ◽  
Stock Analysis ◽  
Mixed Stock

Mitochondrial DNA variation in Drosophila pseudoobscura and related species in Pacific Northwest populations

1985 ◽  
Vol 27 (3) ◽  
pp. 357-364 ◽  
Author(s):  
Lawrence R. Hale ◽  
Andrew T. Beckenbach
Keyword(s):  
Mitochondrial Dna ◽  
Pacific Northwest ◽  
Restriction Enzymes ◽  
The Other ◽  
Drosophila Pseudoobscura ◽  
Rich Region ◽  
Dna Variation ◽  
Restriction Sites ◽  
Dna Restriction ◽  
Tandem Duplications

We have analysed mitochondrial DNA (mtDNA) from Pacific Northwest populations of Drosophila pseudoobscura, D. persimilis, and D. miranda using six restriction enzymes. We find that HpaII restriction sites are hypervariable compared to the other enzymes used. This hypervariability allows construction of a maximum parsimony map linking each mtDNA genotype. Small insertions, possibly tandem duplications, appear to have arisen concomitantly with, or subsequent to, speciation events, perhaps within the A + T rich region. Convergence of mtDNA genotypes is also evident. Unlike findings for other populations of these species, we find little evidence of mitochondrial introgression between D. pseudoobscura and D. persimilis, despite their ability to produce fertile hybrid females.Key words: mitochondrial DNA, restriction endonucleases, Drosophila, evolution.

Bioenergetics and PCB, DDE, and Mercury Dynamics in Lake Ontario Lake Trout (Salvelinus namaycush): A Model based on Surveillance Data

1992 ◽  
Vol 49 (6) ◽  
pp. 1086-1096 ◽  
Author(s):  
U. Borgmann ◽  
D. M. Whittle
Keyword(s):  
Gastrointestinal Tract ◽  
Lake Trout ◽  
Prey Selection ◽  
Lake Ontario ◽  
Salvelinus Namaycush ◽  
Whole Body ◽  
Alosa Pseudoharengus ◽  
Assimilation Rate ◽  
Feeding Rates ◽  
Dynamics Model

Lake trout (Salvelinus namaycush) ingestion rates in a bioenergetics and contaminant dynamics model were estimated directly from contaminant concentrations in lake trout and their prey, rather than from the sum of growth and predicted metabolism. Elimination rates for PCB and DDE, but not for mercury, were dependent on either body mass or lipid content. Concentrations in lake trout responded rapidly to changes in concentration of their prey. This was due primarily to growth dilution and not contaminant elimination, especially for DDE and PCB. Changes in lipid concentrations, therefore, have only minor effects on final concentrations in lake trout, and it is not appropriate to lipid normalize PCB or DDE concentrations when examining trends in whole-body concentrations for this species. Concentrations of PCBs and lipids have declined in lake trout from 1977 to 1988. The drop in PCB concentrations is probably not caused primarily by the lowered lipid concentrations but is the result of either a reduction in feeding rates and improved growth efficiencies, a reduction in PCB concentrations in alewife (Alosa pseudoharengus), or an undocumented change in prey selection. Models based on chemical kinetics across the gastrointestinal tract are more consistent with observed data than models based on a constant contaminant assimilation rate and direct excretion.

Mammalian and teleost cell line bioassay and chemically derived 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalent concentrations in lake trout (Salvelinus namaycush) from Lake Superior and Lake Ontario, North America

1998 ◽  
Vol 17 (11) ◽  
pp. 2214-2226 ◽  
Author(s):  
Jeff J. Whyte ◽  
Mike R. van den Heuvel ◽  
Janine H. Clemons ◽  
Susan Y. Huestis ◽  
Mark R. Servos ◽  
...  
Keyword(s):  
North America ◽  
Cell Line ◽  
Lake Trout ◽  
Lake Superior ◽  
Lake Ontario ◽  
Salvelinus Namaycush ◽  
Tetrachlorodibenzo P Dioxin
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