Construction of a YAC library from barley cultivar Franka and identification of YAC-derived markers linked to the Rh2 gene conferring resistance to scald (Rhynchosporium secalis)

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 1031-1040 ◽  
Author(s):  
Dagmar Schmidt ◽  
Marion S Röder ◽  
Harald Dargatz ◽  
Norbert Wolf ◽  
Günther F Schweizer ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Hordeum Vulgare ◽  
Resistance Gene ◽  
Chromosome 1 ◽  
Rhynchosporium Secalis ◽  
Disease Resistance Gene ◽  
Good Representation ◽  
Artificial Chromosomes ◽  
Entry Points ◽  
Yac Library

The Rh2 resistance gene of barley (Hordeum vulgare) confers resistance against the scald pathogen (Rhynchosporium secalis). A high-resolution genetic map of the Rh2 region on chromosome 1 (7H) was established by the use of molecular markers. Tightly linked markers from this region were used to screen existing and a newly constructed yeast artificial chromosome (YAC) library of barley cv. Franka composed of 45 000 clones representing approximately two genome equivalents. Corresponding YAC clones were identified for most markers, indicating that the combined YAC library has good representation of the barley genome. The contiguous sets of YAC clones with the most tightly linked molecular markers represent entry points for map-based cloning of this resistance gene.Key words: yeast artificial chromosomes, map-based cloning, disease resistance gene, library screening, Hordeum vulgare.

Cloning and Characterization of Disease Resistance Gene Analogs from Poplar (Populus tremula) Chromosome 1

2006 ◽  
Vol 167 (3) ◽  
pp. 403-412 ◽  
Author(s):  
Yong Zhang ◽  
Shougong Zhang ◽  
Liwang Qi ◽  
Bo Liu ◽  
Bingquan Xiong ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Populus Tremula ◽  
Chromosome 1 ◽  
Disease Resistance Gene ◽  
Resistance Gene Analogs

Construction of a MluI-YAC library from barley (Hordeum vulgare L.) and analysis of YAC insert terminal regions

Genome ◽  
1997 ◽  
Vol 40 (6) ◽  
pp. 896-902 ◽  
Author(s):  
Michael Kleine ◽  
Christian Jung ◽  
Wolfgang Michalek ◽  
Thomas Diefenthal ◽  
Harald Dargatz
Keyword(s):  
Hordeum Vulgare ◽  
Gel Electrophoresis ◽  
Yeast Artificial Chromosome ◽  
Single Copy ◽  
Pulsed Field Gel Electrophoresis ◽  
Hordeum Vulgare L ◽  
High Molecular Weight Dna ◽  
Pulsed Field ◽  
Artificial Chromosomes ◽  
Yac Library

We describe the construction of a specific yeast artificial chromosome (YAC) library from barley (Hordeum vulgare L.) using the vector pYAC-RC. The library was generated by total digestion of high molecular weight DNA with the infrequently cutting restriction enzyme MluI. Only 10–30% of the colonies were recombinant, as visualized by red–white selection and subsequent pulsed-field gel electrophoresis analysis. About 17 000 individual recombinant YAC clones with insert sizes ranging from 50 to 700 kb, with a mean of 170 kb, were selected. No chloroplast sequences were detected and the proportion of YAC clones containing BARE–1 copia–like retroelements is about 5%. Screening of the library with a single-copy RFLP marker closely linked to the Mla locus yielded three identical clones of the same size. Insert termini of randomly chosen YAC clones were investigated with respect to their redundancy in the barley genome and compared with termini of YAC clones from an EcoRI-based YAC library, resulting in a fourfold enrichment of single-copy sequences at the MluI vector–insert junctions.Key words: yeast artificial chromosomes, YAC, Hordeum vulgare, pulsed-field gel electrophoresis.

scholarly journals Discovery of powdery mildew resistance gene candidates from Aegilops biuncialis chromosome 2Mb based on transcriptome sequencing

2019 ◽  
Author(s):  
Huanhuan Li ◽  
Zhenjie Dong ◽  
Chao Ma ◽  
Xiubin Tian ◽  
Zhiguo Xiang ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Disease Resistance ◽  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Disease Resistance Gene ◽  
R Genes ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Resistance Gene Candidates

AbstractPowdery mildew is one of the most widespread diseases of wheat. Breeding resistant varieties by utilization of resistance genes is considered as the most economic and effective method of controlling this disease. Previous study showed that the gene(s) at 2Mb in Chinese Spring (CS)-Aegilops biuncialis 2Mb disomic addition line TA7733 conferred high resistance to powdery mildew. In this study, 15 Bgt isolates prevalent in different regions of China were used to further test the resistance spectrum of TA7733. As a result, TA7733 was high resistance to all tested isolates, indicating that the gene(s) on chromosome 2Mb was broad-spectrum powdery mildew resistance. In order to mine resistance gene candidates and develop 2Mb-specific molecular markers to assist the transfer resistance gene(s) at chromosome 2Mb, RNA-seq of TA7733 and CS was conducted before and after Bgt-infection, generating a total of 158,953 unigenes. Of which, 7,278 unigenes were TA7733-specific which were not expressed in CS, and 295 out of these 7,278 unigenes were annotated as R genes. Based on Blastn against with CS Ref Seq v1.0, 61 R genes were further mapped to homoeologous group 2. Analysis of R gene-specific molecular markers designed from R gene sequences verified 40 out of 61 R genes to be 2Mb specific. Annotation of these 40 R genes showed most genes encoded nucleotide binding leucine rich repeat (NLR) protein, being most likely resistance gene candidates. The broad-spectrum powdery mildew resistance gene(s), disease resistance gene candidates, and functional molecular markers of 2Mb-specific in present study will not only lay foundations for transferring disease resistance gene(s) from 2Mb to common wheat by inducing CS-Ae. biuncialis homoeologous recombination, but also provide useful candidates for isolating and cloning resistance gene(s) and dissecting molecular and genetic mechanisms of disease resistance from 2Mb.

scholarly journals Homologues of the Cf-9 Disease Resistance Gene (Hcr9s) Are Present at Multiple Loci on the Short Arm of Tomato Chromosome 1

1999 ◽  
Vol 12 (2) ◽  
pp. 93-102 ◽  
Author(s):  
Martin Parniske ◽  
Brande B. H. Wulff ◽  
Guusje Bonnema ◽  
Colwyn M. Thomas ◽  
David A. Jones ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Milky Way ◽  
Chromosome 1 ◽  
Disease Resistance Gene ◽  
Cladosporium Fulvum ◽  
Large Gene ◽  
Multiple Loci ◽  
Sequence Homologies ◽  
Intergenic Regions ◽  
Complex Locus

The tomato Cf-4 and Cf-9 genes map at a genetically complex locus on the short arm of chromosome 1 and confer resistance against Cladosporium fulvum through recognition of different pathogen-encoded avirulence determinants. Cf-4 and Cf-9 are members of a large gene family (Hcr9s, Homologues of Cladosporium fulvum resistance gene Cf-9), some of which encode additional distinct recognition specificities. A genetic analysis of the majority of Hcr9s suggests that their distribution is spatially restricted to the short arm of chromosome 1. Two loci of clustered Hcr9 genes have been analyzed physically that mapped distal (Northern Lights) and proximal (Southern Cross) to the Cf-4/9 locus (Milky Way). Sequence homologies between intergenic regions at Southern Cross and Milky Way indicate local Hcr9 duplication preceded cluster multiplication. The multiplication of clusters involved DNA flanking Hcr9 sequences as indicated by conserved lipoxy-genase sequences at Southern Cross and Milky Way. The similar spatial distribution of Hcr9 clusters in different Lycopersicon spp. suggests Hcr9 cluster multiplication preceded speciation.

Localization of the rice stripe disease resistance gene, Stv-bi, by graphical genotyping and linkage analyses with molecular markers

1998 ◽  
Vol 96 (8) ◽  
pp. 1044-1049 ◽  
Author(s):  
Y. Hayano-Saito ◽  
T. Tsuji ◽  
K. Fujii ◽  
K. Saito ◽  
M. Iwasaki ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Disease Resistance ◽  
Resistance Gene ◽  
Disease Resistance Gene ◽  
Graphical Genotyping ◽  
Linkage Analyses ◽  
Rice Stripe Disease

scholarly journals A Molecular Cytogenetic Map of Sorghum Chromosome1: Fluorescencein SituHybridization Analysis With Mapped Bacterial Artificial Chromosomes

Genetics ◽  
2002 ◽  
Vol 161 (1) ◽  
pp. 345-353 ◽  
Author(s):  
M N Islam-Faridi ◽  
K L Childs ◽  
P E Klein ◽  
G Hodnett ◽  
M A Menz ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Linkage Map ◽  
Chromosome 1 ◽  
Fish Analysis ◽  
Cytogenetic Map ◽  
Structural Genomic ◽  
Artificial Chromosomes ◽  
Molecular Cytogenetic ◽  
Genomic Resources ◽  
Bac Fish

AbstractWe used structural genomic resources for Sorghum bicolor (L.) Moench to target and develop multiple molecular cytogenetic probes that would provide extensive coverage for a specific chromosome of sorghum. Bacterial artificial chromosome (BAC) clones containing molecular markers mapped across sorghum linkage group A were labeled as probes for fluorescence in situ hybridization (FISH). Signals from single-, dual-, and multiprobe BAC-FISH to spreads of mitotic chromosomes and pachytene bivalents were associated with the largest sorghum chromosome, which bears the nucleolus organizing region (NOR). The order of individual BAC-FISH loci along the chromosome was fully concordant to that of marker loci along the linkage map. In addition, the order of several tightly linked molecular markers was clarified by FISH analysis. The FISH results indicate that markers from the linkage map positions 0.0-81.8 cM reside in the short arm of chromosome 1 whereas markers from 81.8-242.9 cM are located in the long arm of chromosome 1. The centromere and NOR were located in a large heterochromatic region that spans ∼60% of chromosome 1. In contrast, this region represents only 0.7% of the total genetic map distance of this chromosome. Variation in recombination frequency among euchromatic chromosomal regions also was apparent. The integrated data underscore the value of cytological data, because minor errors and uncertainties in linkage maps can involve huge physical regions. The successful development of multiprobe FISH cocktails suggests that it is feasible to develop chromosome-specific “paints” from genomic resources rather than flow sorting or microdissection and that when applied to pachytene chromatin, such cocktails provide an especially powerful framework for mapping. Such a molecular cytogenetic infrastructure would be inherently cross-linked with other genomic tools and thereby establish a cytogenomics system with extensive utility in development and application of genomic resources, cloning, transgene localization, development of plant “chromonomics,” germplasm introgression, and marker-assisted breeding. In combination with previously reported work, the results indicate that a sorghum cytogenomics system would be partially applicable to other gramineous genera.

Exploring Molecular Markers of Preharvest Sprouting Resistance Gene Using Wheat Intact Spikes by Association Analysis

2014 ◽  
Vol 40 (10) ◽  
pp. 1725 ◽  
Author(s):  
Yu-Lei ZHU ◽  
Sheng-Xing WANG ◽  
Liang-Xia ZHAO ◽  
De-Xin ZHANG ◽  
Jian-Bang HU ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Resistance Gene ◽  
Association Analysis ◽  
Preharvest Sprouting ◽  
Sprouting Resistance ◽  
Preharvest Sprouting Resistance
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