Transferability of wheat microsatellites to diploid Aegilops species and determination of chromosomal localizations of microsatellites in the S genome

Genome ◽  
2005 ◽  
Vol 48 (6) ◽  
pp. 959-970 ◽  
Author(s):  
I G Adonina ◽  
E A Salina ◽  
E G Pestsova ◽  
M S Röder
Keyword(s):  
Triticum Aestivum ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Phylogenetic Relationships ◽  
Diploid Species ◽  
Aegilops Speltoides ◽  
Addition Lines ◽  
Chromosome Addition ◽  
Aegilops Longissima ◽  
Chromosome Addition Lines

Overall, 253 genomic wheat (Triticum aestivum) microsatellite markers were studied for their transferability to the diploid species Aegilops speltoides, Aegilops longissima, and Aegilops searsii, representing the S genome. In total, 88% of all the analyzed primer pairs of markers derived from the B genome of hexaploid wheat amplified DNA fragments in the genomes of the studied species. The transferability of simple sequence repeat (SSR) markers of the T. aestivum A and D genomes totaled 74%. Triticum aestivum – Ae. speltoides, T. aestivum – Ae. longissima, and T. aestivum – Ae. searsii chromosome addition lines allowed us to determine the chromosomal localizations of 103 microsatellite markers in the Aegilops genomes. The majority of them were localized to homoeologous chromosomes in the genome of Aegilops. Several instances of nonhomoeologous localization of T. aestivum SSR markers in the Aegilops genome were considered to be either amplification of other loci or putative translocations. The results of microsatellite analysis were used to study phylogenetic relationships among the 3 species of the Sitopsis section (Ae. speltoides, Ae. longissima, and Ae. searsii) and T. aestivum. The dendrogram obtained generally reflects the current views on phylogenetic relationships among these species.Key words: Triticum aestivum, Aegilops speltoides, Aegilops longissima, Aegilops searsii, microsatellite, SSR, chromosome addition lines, phylogeny.

Development of a complete set of Triticum aestivum-Aegilops speltoides chromosome addition lines

2000 ◽  
Vol 101 (1-2) ◽  
pp. 51-58 ◽  
Author(s):  
B. Friebe ◽  
L. L. Qi ◽  
S. Nasuda ◽  
P. Zhang ◽  
N. A. Tuleen ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Aegilops Speltoides ◽  
Addition Lines ◽  
Chromosome Addition ◽  
Chromosome Addition Lines ◽  
Complete Set ◽  
Speltoides Chromosome

The addition of Aegilops variabilis chromosomes to Triticum aestivum and their identification

1983 ◽  
Vol 25 (1) ◽  
pp. 76-84 ◽  
Author(s):  
D. C. Jewell ◽  
C. J. Driscoll
Keyword(s):  
Triticum Aestivum ◽  
Triticum Aestivum L ◽  
Addition Lines ◽  
Single Chromosome ◽  
Aegilops Umbellulata ◽  
Chromosome Addition ◽  
Aegilops Longissima ◽  
Chromosome Addition Lines ◽  
Aegilops Variabilis ◽  
The Relationship

Nine of the 14 possible single chromosome addition lines of the tetraploid species Aegilops variabilis Eig. (CuCuSvSv) to Triticum aestivum L. cv. Chinese Spring (AA BB DD) have been isolated and identified. The nine Aegilops variabilis addition lines were compared with the available addition lines of Aegilops umbellulata (CuCu) and Aegilops longissima (SvSv) to further elucidate the relationship between these two diploids and the tetraploid Aegilops variabilis. Differences were observed between the same chromosomes isolated from the diploid and the tetraploid and discussed. After taking into account banding pattern polymorphisms, Aegilops umbellulata was confirmed as the donor of the Cu genome, and evidence indicated that Aegilops longissima probably is the donor of the other genome (Sv) in the tetraploid Aegilops variabilis.

Cytogenetic identification of Triticum peregrinum chromosomes added to common wheat

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 272-276 ◽  
Author(s):  
B. Friebe ◽  
E. D. Badaeva ◽  
B. S. Gill ◽  
N. A. Tuleen
Keyword(s):  
Triticum Aestivum ◽  
Key Words ◽  
Common Wheat ◽  
Addition Lines ◽  
Karyotypic Analysis ◽  
Chromosome Addition ◽  
C Banding ◽  
Chromosome Addition Lines ◽  
Complete Set ◽  
Donor Species

C-banded karyotypes of a complete set of 14 Triticum peregrinum whole chromosome addition lines and 25 telosomic addition lines are reported. The added T. peregrinum chromosomes were not structurally rearranged compared with the corresponding chromosomes of the donor accession. Comprehensive karyotypic analysis confirmed Triticum umbellulatum as the donor species of the Uv genome and identified Triticum longissimum as the donor species of the Sv genome of T. peregrinum. Neither the Uv nor Sv genome chromosomes of the T. peregrinum accession showed large modifications when compared with the ancestral U and S1 genomes. Key words : Triticum aestivum, Triticum peregrinum, Triticum umbellulatum, Triticum longissimum, chromosome addition lines, C-banding.

Development and identification of a complete set of Triticum aestivum - Aegilops geniculata chromosome addition lines

Genome ◽  
1999 ◽  
Vol 42 (3) ◽  
pp. 374-380 ◽  
Author(s):  
Bernd R Friebe ◽  
Neal A Tuleen ◽  
Bikram S Gill
Keyword(s):  
Triticum Aestivum ◽  
Chromosome Breakage ◽  
Meiotic Pairing ◽  
Addition Lines ◽  
Aegilops Geniculata ◽  
Chromosome Addition ◽  
C Banding ◽  
Chromosome Addition Lines ◽  
Complete Set ◽  
Gametocidal Gene

The production and identification of a complete set of intact Aegilops geniculata chromosome and telosome additions to common wheat is described. All Ug and Mg genome chromosomes were tentatively assigned to their homoeologous groups based on C-banding, meiotic metaphase I pairing analyses and plant morphologies. Thirteen disomic and one monosomic wheat-Ae. geniculata chromosome additions were identified. Furthermore, two monotelosomic (MtA7UgL, MtA7MgL) and nine ditelosomic (DtA1UgS, DtA1UgL, DtA2UgS, DtA1MgL, DtA2MgL, DtA3MgS, DtA5MgS, DtA6MgL, DtA7MgS) wheat-Ae. geniculata additions were recovered. C-banding and meiotic pairing analyses revealed that all added Ug and Mg genome chromosomes are structurally unaltered compared to the Ae. geniculata parent accession. Chromosome 4Mg has a strong gametocidal gene that, when transferred to wheat, causes extensive chromosome breakage mainly in gametes lacking it. The relationships of Ae. geniculata chromosomes with those of the diploid progenitor species and derived polyploids is discussed.Key words: Triticum aestivum, Aegilops geniculata, chromosome addition lines, C-banding, genome evolution.

THE ADDITION OF SINGLE CHROMOSOMES OF AVENA HIRTULA TO THE CULTIVATED HEXAPLOID OAT A. SATIVA

1968 ◽  
Vol 10 (3) ◽  
pp. 551-563 ◽  
Author(s):  
Hugh Thomas
Keyword(s):  
Chromosome Number ◽  
Diploid Species ◽  
Addition Lines ◽  
Single Chromosome ◽  
Chromosome Addition ◽  
Chromosome Addition Lines ◽  
A Genome ◽  
Disomic Addition Lines ◽  
Diploid Level ◽  
Wild Diploid Species

Six of the possible seven single chromosome addition lines of the wild diploid species A. hirtula to the cultivated oat A. sativa have been identified. The effect of the single hirtula chromosome on the morphology of the recipient A. sativa variety Manod was variable depending on the chromosome involved and certain genes which are dominant at the diploid level were only partly expressed in the hexaploid background.The frequency with which the hirtula chromosomes paired with their equivalent chromosomes in A. sativa was less than that observed in primary trisomics, indicating that the hirtula As genome is only partly homologous with the A genome of the hexaploids. None of the disomic addition lines was sufficiently stable cytologically to maintain the line without the reversion of a proportion of the progeny to the monosomic condition and eventually to the euploid chromosome number of A. sativa.

Development and chromosomal localization of genome-specific DNA markers of Brassica and the evolution of amphidiploids and n = 9 diploid species

Genome ◽  
1990 ◽  
Vol 33 (1) ◽  
pp. 131-142 ◽  
Author(s):  
K. Hosaka ◽  
S. F. Kianian ◽  
J. M. McGrath ◽  
C. F. Quiros
Keyword(s):  
Marker Chromosome ◽  
Diploid Species ◽  
Addition Lines ◽  
Chromosome Assignment ◽  
Chromosome Addition ◽  
Dna Libraries ◽  
Chromosome Addition Lines ◽  
Limited Variation ◽  
Partial Homology ◽  
Alien Addition Lines

Ten genome-specific probes were developed from Brassica napus and B. oleracea genomic DNA libraries. Selection was based on polymorphism between and limited variation within genomes, permitting their localization on six individual C-genome chromosomes. Chromosome assignment was accomplished by using two sets of B. campestris – oleracea alien addition lines derived from (i) B. napus and (ii) the artificially synthesized B. napus 'Hakuran'. The presence of shared fragments between A, B, and C genomes indicates partial homology of the three genomes. However, several genome-specific markers could separate these three genomes. Genome-specific clones developed in this study served to confirm the parental diploid species originating the three amphidiploids, B. napus, B. carinata, and B. juncea. At least one clone suggests that B. napus has a polyphyletic origin. These clones were also useful to confirm the close evolutionary proximity among wild species in the B. oleracea cytodeme; however, no clear trends were found to suggest specific wild ancestors for the different B. oleracea horticultural types. Brassica oxyrrhina was distinct from other n = 9 species with most clones tested.Key words: restriction fragment length polymorphism, DNA marker, chromosome addition lines, Brassica oleracea cytodeme, amphidiploids, genome-specific markers.

Sign in / Sign up

Export Citation Format

Share Document