Analysis of nucleolar activity in Agropyron elongatum, its amphiploid with Triticum aestivum and the chromosome addition lines

1984 ◽  
Vol 68-68 (1-2) ◽  
pp. 75-80 ◽  
Author(s):  
J. R. Lacadena ◽  
M. C. Cermeño ◽  
J. Orellana ◽  
J. L. Santos
Keyword(s):  
Triticum Aestivum ◽  
Addition Lines ◽  
Agropyron Elongatum ◽  
Nucleolar Activity ◽  
Chromosome Addition ◽  
Chromosome Addition Lines

Transferability of wheat microsatellites to diploid Aegilops species and determination of chromosomal localizations of microsatellites in the S genome

Genome ◽  
2005 ◽  
Vol 48 (6) ◽  
pp. 959-970 ◽  
Author(s):  
I G Adonina ◽  
E A Salina ◽  
E G Pestsova ◽  
M S Röder
Keyword(s):  
Triticum Aestivum ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Phylogenetic Relationships ◽  
Diploid Species ◽  
Aegilops Speltoides ◽  
Addition Lines ◽  
Chromosome Addition ◽  
Aegilops Longissima ◽  
Chromosome Addition Lines

Overall, 253 genomic wheat (Triticum aestivum) microsatellite markers were studied for their transferability to the diploid species Aegilops speltoides, Aegilops longissima, and Aegilops searsii, representing the S genome. In total, 88% of all the analyzed primer pairs of markers derived from the B genome of hexaploid wheat amplified DNA fragments in the genomes of the studied species. The transferability of simple sequence repeat (SSR) markers of the T. aestivum A and D genomes totaled 74%. Triticum aestivum – Ae. speltoides, T. aestivum – Ae. longissima, and T. aestivum – Ae. searsii chromosome addition lines allowed us to determine the chromosomal localizations of 103 microsatellite markers in the Aegilops genomes. The majority of them were localized to homoeologous chromosomes in the genome of Aegilops. Several instances of nonhomoeologous localization of T. aestivum SSR markers in the Aegilops genome were considered to be either amplification of other loci or putative translocations. The results of microsatellite analysis were used to study phylogenetic relationships among the 3 species of the Sitopsis section (Ae. speltoides, Ae. longissima, and Ae. searsii) and T. aestivum. The dendrogram obtained generally reflects the current views on phylogenetic relationships among these species.Key words: Triticum aestivum, Aegilops speltoides, Aegilops longissima, Aegilops searsii, microsatellite, SSR, chromosome addition lines, phylogeny.

Cytogenetic identification of Triticum peregrinum chromosomes added to common wheat

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 272-276 ◽  
Author(s):  
B. Friebe ◽  
E. D. Badaeva ◽  
B. S. Gill ◽  
N. A. Tuleen
Keyword(s):  
Triticum Aestivum ◽  
Key Words ◽  
Common Wheat ◽  
Addition Lines ◽  
Karyotypic Analysis ◽  
Chromosome Addition ◽  
C Banding ◽  
Chromosome Addition Lines ◽  
Complete Set ◽  
Donor Species

C-banded karyotypes of a complete set of 14 Triticum peregrinum whole chromosome addition lines and 25 telosomic addition lines are reported. The added T. peregrinum chromosomes were not structurally rearranged compared with the corresponding chromosomes of the donor accession. Comprehensive karyotypic analysis confirmed Triticum umbellulatum as the donor species of the Uv genome and identified Triticum longissimum as the donor species of the Sv genome of T. peregrinum. Neither the Uv nor Sv genome chromosomes of the T. peregrinum accession showed large modifications when compared with the ancestral U and S1 genomes. Key words : Triticum aestivum, Triticum peregrinum, Triticum umbellulatum, Triticum longissimum, chromosome addition lines, C-banding.

Development of a complete set of Triticum aestivum-Aegilops speltoides chromosome addition lines

2000 ◽  
Vol 101 (1-2) ◽  
pp. 51-58 ◽  
Author(s):  
B. Friebe ◽  
L. L. Qi ◽  
S. Nasuda ◽  
P. Zhang ◽  
N. A. Tuleen ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Aegilops Speltoides ◽  
Addition Lines ◽  
Chromosome Addition ◽  
Chromosome Addition Lines ◽  
Complete Set ◽  
Speltoides Chromosome

Isolation and characterization of wheat–Elytrigia elongata chromosome 3E and 5E addition and substitution lines

Genome ◽  
1988 ◽  
Vol 30 (4) ◽  
pp. 519-524 ◽  
Author(s):  
N. A. Tuleen ◽  
G. E. Hart
Keyword(s):  
Chinese Spring ◽  
Addition Lines ◽  
Homoeologous Group ◽  
Substitution Lines ◽  
Agropyron Elongatum ◽  
Chromosome Addition ◽  
Isolation And Characterization ◽  
Chromosome Addition Lines ◽  
Group 3 ◽  
Group 5

Isozyme markers were used to develop Triticum aestivum cv. Chinese Spring–Elytrigia elongata (= Agropyron elongatum, 2n = 14, genome E) disomic 3E and 5E addition lines. Subsequently, all possible lines containing 3E and 5E substituted for wheat homoeologues and several 3E and 5E ditelosomic addition and substitution lines were developed. Plants containing chromosome 3E substituted for wheat chromosomes of homoeologous group 3 are similar to 'Chinese Spring' in vigor and fertility while plants containing 3EL substituted for chromosomes of group 3 are less fertile than 'Chinese Spring'. This indicates that both arms of 3E are involved in sporophytic compensation. Plants containing chromosome 5E substituted for wheat chromosomes of homoeologous group 5 are as vigorous but less fertile than 'Chinese Spring'. 5EL (5A) and 5EL (5B) plants are lower in fertility than 5E (5A) and 5E (5B) plants, indicating that both arms of 5E are involved in sporophytic compensation. 5E (5D) and 5EL (5D) plants are similar in fertility. Male gametophytes in which 3E or 5E replaces a wheat homoeologue function at a lower rate than normal gametes.Key words: wheat, Triticum, Elytrigia elongata, alien chromosome addition lines.

scholarly journals Chromosomal locations of eleven Elytrigia elongata (= Agropyron elongatum) isozyme structural genes

1983 ◽  
Vol 41 (2) ◽  
pp. 181-202 ◽  
Author(s):  
Gary E. Hart ◽  
Neal A. Tuleen
Keyword(s):  
Chinese Spring ◽  
Common Ancestor ◽  
Addition Lines ◽  
Structural Genes ◽  
Agropyron Elongatum ◽  
Chromosome Addition ◽  
Chromosome Addition Lines ◽  
Study Support ◽  
Disomic Addition Lines ◽  
Valuable Role

SUMMARYThe zymogram phenotypes of 11 enzymes were determined for 22 Triticum aestivum cv. Chinese Spring-Elytrigia elongata disomic and ditelosomic chromosome addition lines. Eleven isozyme structural genes were located in specific arms of six E. elongata chromosomes, as follows: Gpi-E1 in 1ES, Est-E1 in 3ES, Got-E3 in 3EL, Adh-E1 and Lpx-E1 in 4ES, Adh-E2 and Lpx-E2 in 5EL, Amp-E1 in 6Eα, Adh-E3 and Got-E2 in 6Eβ, and Ep-E1 in 7EL. The E. elongata chromosomes present in five disomic addition lines have previously been designated 1E, 2E, 4E, 6E, and 7E to indicate their homoeology with Chinese Spring chromosomes. The results of this study support these designations. The development of disomic putative 3E and 5E addition lines is reported. The added chromosomes designated IV, V, and VI that are present in three of the seven original disomic T. aestivum-E. elongata addition lines are translocated. Evidence that VL and VIL are opposite arms of 2E and that IV is partially homoeologous to 3E has been published. The results reported in this paper indicate that IVS = 3ES, IVL = 7EL, VS = 3ES, and VIS = 5ES and are consistent with VL and VIL being opposite arms of 2E. The synteny relationships of the 11 E. elongata isozyme genes identified in this study are fully consistent with those of homoeologous T. aestivum cv. Chinese Spring genes and thus provide evidence that the gene synteny groups which these two species inherited from their common ancestor are conserved. This study further documents the valuable role that studies of isozyme genes can play in the isolation, characterization, and maintenance of alien chromosomes, telosomes, and chromosomal segments in wheat strains.

The addition of Aegilops variabilis chromosomes to Triticum aestivum and their identification

1983 ◽  
Vol 25 (1) ◽  
pp. 76-84 ◽  
Author(s):  
D. C. Jewell ◽  
C. J. Driscoll
Keyword(s):  
Triticum Aestivum ◽  
Triticum Aestivum L ◽  
Addition Lines ◽  
Single Chromosome ◽  
Aegilops Umbellulata ◽  
Chromosome Addition ◽  
Aegilops Longissima ◽  
Chromosome Addition Lines ◽  
Aegilops Variabilis ◽  
The Relationship

Nine of the 14 possible single chromosome addition lines of the tetraploid species Aegilops variabilis Eig. (CuCuSvSv) to Triticum aestivum L. cv. Chinese Spring (AA BB DD) have been isolated and identified. The nine Aegilops variabilis addition lines were compared with the available addition lines of Aegilops umbellulata (CuCu) and Aegilops longissima (SvSv) to further elucidate the relationship between these two diploids and the tetraploid Aegilops variabilis. Differences were observed between the same chromosomes isolated from the diploid and the tetraploid and discussed. After taking into account banding pattern polymorphisms, Aegilops umbellulata was confirmed as the donor of the Cu genome, and evidence indicated that Aegilops longissima probably is the donor of the other genome (Sv) in the tetraploid Aegilops variabilis.

scholarly journals The genetic control of hexokinase isozymes in wheat

1983 ◽  
Vol 42 (2) ◽  
pp. 219-227 ◽  
Author(s):  
C. C. Ainsworth
Keyword(s):  
Genetic Control ◽  
Chinese Spring ◽  
Glucose Phosphate Isomerase ◽  
Addition Lines ◽  
Agropyron Elongatum ◽  
Wheat Grains ◽  
Chromosome Addition ◽  
Glucose Phosphate ◽  
Chromosome Addition Lines

SUMMARYIn extracts of mature wheat grains, 13 hexokinase isozymes were distinguished by IEF. The genes controlling the production of five isozymes were located on chromosome arms 1BS, 1DS and 3BS by nullisomic analysis. The three loci, part of two homoeoallelic series (Hk-1 and Hk-2) are designated Hk-B1, Hk-D1 and Hk-B2 respectively. Analysis of chromosome 1D short-arm terminal deletions indicated the Hk-D1 locus to be located proximally to the glucose phosphate isomerase locus, Gpi-D1 on the shortarm. Three variant HK phenotypes were distinguished amongst 55 hexaploid wheats examined. Analysis of seven Chinese Spring/Agropyron elongatum chromosome addition lines showed that Ag. elongatum isozymes were expressed in the wheat background in additions IV and V.

Development and identification of a complete set of Triticum aestivum - Aegilops geniculata chromosome addition lines

Genome ◽  
1999 ◽  
Vol 42 (3) ◽  
pp. 374-380 ◽  
Author(s):  
Bernd R Friebe ◽  
Neal A Tuleen ◽  
Bikram S Gill
Keyword(s):  
Triticum Aestivum ◽  
Chromosome Breakage ◽  
Meiotic Pairing ◽  
Addition Lines ◽  
Aegilops Geniculata ◽  
Chromosome Addition ◽  
C Banding ◽  
Chromosome Addition Lines ◽  
Complete Set ◽  
Gametocidal Gene

The production and identification of a complete set of intact Aegilops geniculata chromosome and telosome additions to common wheat is described. All Ug and Mg genome chromosomes were tentatively assigned to their homoeologous groups based on C-banding, meiotic metaphase I pairing analyses and plant morphologies. Thirteen disomic and one monosomic wheat-Ae. geniculata chromosome additions were identified. Furthermore, two monotelosomic (MtA7UgL, MtA7MgL) and nine ditelosomic (DtA1UgS, DtA1UgL, DtA2UgS, DtA1MgL, DtA2MgL, DtA3MgS, DtA5MgS, DtA6MgL, DtA7MgS) wheat-Ae. geniculata additions were recovered. C-banding and meiotic pairing analyses revealed that all added Ug and Mg genome chromosomes are structurally unaltered compared to the Ae. geniculata parent accession. Chromosome 4Mg has a strong gametocidal gene that, when transferred to wheat, causes extensive chromosome breakage mainly in gametes lacking it. The relationships of Ae. geniculata chromosomes with those of the diploid progenitor species and derived polyploids is discussed.Key words: Triticum aestivum, Aegilops geniculata, chromosome addition lines, C-banding, genome evolution.

Sign in / Sign up

Export Citation Format

Share Document