Genomic instability in Solanum tuberosum × Solanum kurtzianum interspecific hybrids

Genome ◽  
2006 ◽  
Vol 49 (2) ◽  
pp. 104-113 ◽  
Author(s):  
C F Marfil ◽  
R W Masuelli ◽  
J Davison ◽  
L Comai
Keyword(s):  
Dna Methylation ◽  
Solanum Tuberosum ◽  
Interspecific Hybrids ◽  
Genome Instability ◽  
Solanum Tuberosum L ◽  
Interspecific Crosses ◽  
Genomic Changes ◽  
Variation Patterns ◽  
Solanum Kurtzianum ◽  
Methylation Patterns

The use of interspecific crosses in breeding is an important strategy in improving the genetic base of the modern cultivated potato, Solanum tuberosum L. Until now, it has normally been interspecific Solanum hybrids that have been morphologically and cytologically characterized. However, little is known about the genomic changes that may occur in the hybrid nucleus owing to the combination of genomes of different origin. We have observed novel AFLP bands in Solanum tuberosum × Solanum kurtzianum diploid hybrids; 40 novel fragments were detected out of 138 AFLP fragments analyzed. No cytological abnormalities were observed in the hybrids; however, we found DNA methylation changes that could be the cause of the observed genomic instabilities. Of 277 MSAP fragments analyzed, 14% showed methylation patterns that differed between the parental species and the hybrids. We also observed frequent methylation changes in the BC1 progeny. Variation patterns among F1 and BC1 plants suggest that some methylation changes occurred at random. The changes observed may have implications for potato breeding as an additional source of variability.Key words: DNA methylation, genome instability, interspecific hybrids, potato, Solanum.

scholarly journals Genomic Outcomes of Haploid Induction Crosses in Potato (Solanum tuberosum L.)

Genetics ◽  
2019 ◽  
Vol 214 (2) ◽  
pp. 369-380 ◽  
Author(s):  
Kirk R. Amundson ◽  
Benny Ordoñez ◽  
Monica Santayana ◽  
Ek Han Tan ◽  
Isabelle M. Henry ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Genetic Markers ◽  
Large Scale ◽  
Solanum Tuberosum L ◽  
Small Scale ◽  
Haploid Induction ◽  
Variation Patterns ◽  
Number Variation ◽  
Additional Chromosome ◽  
Chromosomal Loci

The challenges of breeding autotetraploid potato (Solanum tuberosum) have motivated the development of alternative breeding strategies. A common approach is to obtain uniparental dihaploids from a tetraploid of interest through pollination with S. tuberosum Andigenum Group (formerly S. phureja) cultivars. The mechanism underlying haploid formation of these crosses is unclear, and questions regarding the frequency of paternal DNA transmission remain. Previous reports have described aneuploid and euploid progeny that, in some cases, displayed genetic markers from the haploid inducer (HI). Here, we surveyed a population of 167 presumed dihaploids for large-scale structural variation that would underlie chromosomal addition from the HI, and for small-scale introgression of genetic markers. In 19 progeny, we detected 10 of the 12 possible trisomies and, in all cases, demonstrated the noninducer parent origin of the additional chromosome. Deep sequencing indicated that occasional, short-tract signals appearing to be of HI origin were better explained as technical artifacts. Leveraging recurring copy number variation patterns, we documented subchromosomal dosage variation indicating segregation of polymorphic maternal haplotypes. Collectively, 52% of the assayed chromosomal loci were classified as dosage variable. Our findings help elucidate the genomic consequences of potato haploid induction and suggest that most potato dihaploids will be free of residual pollinator DNA.

DNA Methylation in Placentas of Interspecies Mouse Hybrids

Genetics ◽  
2003 ◽  
Vol 165 (1) ◽  
pp. 223-228
Author(s):  
Sabine Schütt ◽  
Andrea R Florl ◽  
Wei Shi ◽  
Myriam Hemberger ◽  
Annie Orth ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Molecular Basis ◽  
Interspecific Hybrids ◽  
Genetic System ◽  
Differential Methylation ◽  
Late Gestation ◽  
Epigenetic Changes ◽  
Repeat Sequences ◽  
Mouse Placenta ◽  
Methylation Patterns

Abstract Interspecific hybridization in the genus Mus results in several hybrid dysgenesis effects, such as male sterility and X-linked placental dysplasia (IHPD). The genetic or molecular basis for the placental phenotypes is at present not clear. However, an extremely complex genetic system that has been hypothesized to be caused by major epigenetic changes on the X chromosome has been shown to be active. We have investigated DNA methylation of several single genes, Atrx, Esx1, Mecp2, Pem, Psx1, Vbp1, Pou3f4, and Cdx2, and, in addition, of LINE-1 and IAP repeat sequences, in placentas and tissues of fetal day 18 mouse interspecific hybrids. Our results show some tendency toward hypomethylation in the late gestation mouse placenta. However, no differential methylation was observed in hyper- and hypoplastic hybrid placentas when compared with normal-sized littermate placentas or intraspecific Mus musculus placentas of the same developmental stage. Thus, our results strongly suggest that generalized changes in methylation patterns do not occur in trophoblast cells of such hybrids.

Morphological and molecular evidence of natural interspecific hybridization in the diploid potato Solanum kurtzianum from Argentina

Botany ◽  
2009 ◽  
Vol 87 (1) ◽  
pp. 78-87 ◽  
Author(s):  
María Cecilia Bedonni ◽  
Elsa Lucila Camadro
Keyword(s):  
Interspecific Hybridization ◽  
Solanum Tuberosum L ◽  
A Priori ◽  
Diploid Species ◽  
Morphological Plasticity ◽  
Solanum Chacoense ◽  
Breeding Value ◽  
Interspecific Crosses ◽  
Molecular Evidence ◽  
Solanum Kurtzianum

The common potato, Solanum tuberosum L. subsp. tuberosum , is a tetrasomic tetraploid. This subspecies has approximately 220 related tuber-bearing species that constitute a genetic pool of enormous breeding value. The diploid species Solanum kurtzianum Bitter et Wittm., described as resistant to drought and nematodes, grows in northwestern and western Argentina and can naturally hybridize with the related diploids Solanum chacoense Bitter, Solanum spegazzinii Bitter, and Solanum maglia Schltdl., in areas of sympatry. In this species, the variability for morphological phenotypes and pollen–pistil relations in intra- and interspecific crosses is large. To investigate whether S. kurtzianum accessions available at the Germplasm Bank in Argentina belong to one taxon with great morphological plasticity or are hybrid and (or) segregant populations, these and accessions of the other three species were characterized using morphological and molecular (SSR) markers. Data were statistically analyzed with ANOVAs and AMOVA. With univariate ANOVA, only 11 characters out of 33 evaluated were statistically significant among species. With multivariate analyses, the grouping did not reflect a priori taxonomic assignments; when population averages were used, a tendency was observed towards grouping by geographical origin. With the AMOVA, only individuals within accessions were separated. Interspecific hybridization and introgression in areas of sympatry can explain these results.

Situación económica y social de la población de San José del Carmen, San Cristóbal de las Casas, Chiapas

2019 ◽  
Vol 12 (4) ◽  
Author(s):  
Pedro Cadena-Iñiguez ◽  
Eileen Salinas-Cruz ◽  
Jesús Martínez-Sánchez ◽  
Mariano Morales-Guerra ◽  
Romualdo Vásquez-Ortiz ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Theobroma Cacao ◽  
Zea Mays L ◽  
Solanum Tuberosum L ◽  
San Jose ◽  
Phaseolus Vulgaris L ◽  
Las Casas ◽  
Vicia Faba L ◽  
Las Mujeres ◽  
Theobroma Cacao L

Objetivo: Establecer una línea base como fundamento para la intervención e inducción de innovaciones a través de escuelas de campo y planes de negocios. Diseño/metodología/aproximación: El estudio se desarrolló en San José del Carmen, San Cristóbal de Las Casas, Chiapas, México. Se elaboró una encuesta como instrumento para recabar en campo información de familias de la localidad con la finalidad de conocer su situación actual. La localidad de trabajo se eligió utilizando como criterio las poblaciones que se encuentran dentro de Cruzada Nacional contra el hambre y en la clasificación de la pobreza extrema Resultados: La localidad de San José del Carmen es considerada como de alta marginación, la población es bilingüe, su principal lengua es el Tzotzil, el nivel de estudios promedio es hasta el tercer año de primaria, las principales actividades productivas son la siembra de maíz (Zea mays L.), frijol (Phaseolus vulgaris L.), papa (Solanum tuberosum L.) y haba (Vicia faba L.) que son utilizadas principalmente para autoconsumo.  No cuentan con agua potable ya que su sistema es a través de la recaudación de agua de lluvia o a través de un jagüey. Sus principales actividades económicas son la albañilería por parte de los hombres, y venta de productos, tales como el pozol (bebida energética a base de maíz y cacao (Theobroma cacao L.), tostadas y algunos vegetales por parte de las mujeres. Las tostadas son producidas en forma artesanal con un proceso de doble nixtamalización y vendida en bolsas de 20 tostadas cada una, a un costo de MX$10.00 en los mercados de San Cristóbal de las Casas, una a dos veces por semana alrededor de 40 bolsas por día de venta. Limitaciones del estudio/implicaciones: La afiliación zapatista de alguno de los pobladores y el hecho de que algunos habitantes no hablen español, fueron factores que limitaron desarrollar al 100% el estudio; sin embargo, se considera que se tienen los elementos necesarios para establecer la línea base de la situación de San José del Carmen. Conclusiones: El diagnóstico servirá como una herramienta para establecer una intervención a través de innovaciones y planes de negocios en la comunidad de estudio. Los pobladores cuentan con herramientas necesarias para obtener nuevos conocimientos que ayuden a un desarrollo de su producción. El idioma no deberá de ser una limitante para ello.

ALLELE DIVERSITY FOR ACID VACUOLAR INVERTASE GENE Pain-1 FRAGMENT IN POTATO (Solanum tuberosum L.) VARIETIES AND LINES

2018 ◽  
Vol 53 (1) ◽  
pp. 132-139
Author(s):  
M.A. Slugina ◽  
◽  
E.O. Shmelkova ◽  
A.A. Meleshin ◽  
E.Z. Kochieva ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Vacuolar Invertase ◽  
Invertase Gene
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