Genetic analysis and mapping of a thermosensitive genic male sterility gene, tms6(t), in rice (Oryza sativa L.)

Genome ◽  
2010 ◽  
Vol 53 (2) ◽  
pp. 119-124 ◽  
Author(s):  
Xia Liu ◽  
Xihong Li ◽  
Xin Zhang ◽  
Songwen Wang
Keyword(s):  
Genetic Analysis ◽  
Molecular Mapping ◽  
Oryza Sativa L ◽  
Bulked Segregant Analysis ◽  
Recessive Gene ◽  
Male Sterile ◽  
Chromosome 10 ◽  
Physical Maps ◽  
Specific Temperature ◽  
Simple Sequence

Thermosensitive genic male-sterile (TGMS) rice is widely used in hybrid rice production. Because of its specific temperature requirement, it can be used only in a narrow rice-growing zone in Asia. A newly discovered TGMS line, G20S, has an opposite phenotype compared with normal TGMS lines. G20S is completely sterile when the temperature is lower than 29.5 °C. Thus, it can be widely used in a larger area. Genetic analysis indicated that the sterility of G20S was controlled by a single recessive gene that was named tms6(t). An F2 population from the cross between G20S and Jing226 was developed and used for molecular mapping of the tms6(t) gene. The simple sequence repeat technique, combined with bulked segregant analysis, was used to screen markers linked to the target gene, and linkage markers such as RM216, RM2504, RM3152, and RM4455 were identified. These markers are all located on chromosome 10; therefore, tms6(t) was subsequently mapped on chromosome 10. Co-segregation analysis using the F2 population showed that two of these markers, RM3152 and RM4455, were closely linked to tms6(t) at distances of 3.00 cM and 1.10 cM, respectively. Additionally, integration of the genetic and physical maps showed that there were 15 BAC/PAC clones overlapping between RM3152 and RM4455, and tms6(t) was mapped to an interval of 1455 kb.

Fine mapping of a pistilloid-stamen (PS) gene on the short arm of chromosome 1 in rice

Genome ◽  
2006 ◽  
Vol 49 (8) ◽  
pp. 1016-1022 ◽  
Author(s):  
Hongfa Luo ◽  
Yunfeng Li ◽  
Zhenglin Yang ◽  
Bingqiang Zhong ◽  
Rong Xie ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Simple Sequence Repeat ◽  
Molecular Mapping ◽  
Oryza Sativa L ◽  
Recessive Gene ◽  
Physical Region ◽  
Floral Organ ◽  
Chromosome 1 ◽  
Sequence Repeat ◽  
Simple Sequence

A novel floral organ mutant of rice (Oryza sativa L. subsp. indica), termed pistilloid-stamen (ps) here, has flowers with degenerated lemma and palea, with some stamens transformed into pistils and pistil–stamen chimeras. Genetic analysis confirmed that the ps trait is controlled by a single recessive gene. F2 and F3 segregation populations derived from PS ps heterozygote crossed with Oryza sativa subsp. indica 'Luhui-17' (PS PS) were used for molecular mapping of the gene using simple sequence repeat (SSR) markers. With 97 recessive individuals from an F2 segregation population, the ps locus was preliminarily mapped 6.2 cM distal to marker RM6324 and 3.1 cM proximal to marker RM6340 in the terminal region of the short arm of chromosome 1. With a large F3 segregation population, the gene was fine-mapped between markers RM6470 and RM1141, at distances of 0.10 and 0.03 cM to each marker, respectively. The position of the ps gene was finally located within a 20 kb physical region containing 3 annotated putative genes. One of them, encoding a protein with a single C2H2 zinc-finger domain, may be the candidate gene for PS.Key words: rice (Oryza sativa L. subsp. indica), pistilloid-stamen mutant (ps mutant), molecular marker, simple sequence repeat (SSR), gene mapping

Phenotypic characterization, genetic analysis, and molecular mapping of a new mutant gene for male sterility in rice

Genome ◽  
2008 ◽  
Vol 51 (4) ◽  
pp. 303-308 ◽  
Author(s):  
Ling Zuo ◽  
Shuangcheng Li ◽  
Mingguang Chu ◽  
Shiquan Wang ◽  
Qiming Deng ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Molecular Mapping ◽  
Spontaneous Mutation ◽  
Recessive Gene ◽  
Pollen Grains ◽  
Mutant Gene ◽  
Phenotypic Characterization ◽  
Chromosome 4 ◽  
Male Sterile ◽  
Rice Mutant

xs1 is a male-sterile rice mutant derived from a spontaneous mutation. The floret of the mutant, consisting of 6 stamens and 1 pistil, looks the same as that of the wild type except that the filaments are long and thin and the anthers are withered in white transparence. It is confirmed that xs1 is a no-pollen type of male-sterile mutant, for no pollen grains can be stained with I2–KI solution and the anther locules are always hollow. Anther transverse sections indicate that the mutant microspores are abnormally condensed and agglomerated to form a deeply stained cluster at the late microspore stage, which results in cessation of the vacuolation process of microspores, and, therefore, the mutant forms no functional pollens for reproduction. Genetic analysis of 4 F2 populations and 3 BC1F1 populations revealed that the mutation is controlled by a single recessive gene, termed VR1 (Vacuolation retardation 1). Screening of 432 F2 mutant individuals derived from the cross of xs1 × G603 with simple sequence repeat markers revealed that VR1 is located between the molecular markers RM17411 and RM5030, at distances of 0.7 and 1.5 cM, respectively, on chromosome 4. VR1 is a new male fertility controlling gene located on chromosome 4 in rice.

Genetic Analysis and Molecular Mapping of a Male Sterile Mutant in Rice

2009 ◽  
Vol 35 (6) ◽  
pp. 1151-1155
Author(s):  
Ming-Guang CHU ◽  
Shuang-Cheng LI ◽  
Shi-Quan WANG ◽  
Qi-Ming DENG ◽  
Jing ZHANG ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Molecular Mapping ◽  
Male Sterile ◽  
Sterile Mutant

Genetic Analysis and Molecular Mapping of a Rolled Leaf Gene RL13in Rice(Oryza sativa L.)

2012 ◽  
Vol 38 (3) ◽  
pp. 423
Author(s):  
Xiao-Qing TIAN ◽  
Xian-Chun SANG ◽  
Fang-Ming ZHAO ◽  
Yun-Feng LI ◽  
Ying-Hua LING ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Genetic Analysis ◽  
Molecular Mapping ◽  
Oryza Sativa L ◽  
Rolled Leaf

scholarly journals INHERITANCE OF FERTILITY RESTORATION INVOLVING WILD ABORTIVE CYTOPLASMIC MALE STERILITY SYSTEM IN RICE (Oryza sativa L.)

2011 ◽  
Vol 24 (1) ◽  
pp. 33-40
Author(s):  
M. J. Hasan ◽  
M. U. Kulsum ◽  
A. Ansari ◽  
A. K. Paul ◽  
P. L. Biswas
Keyword(s):  
Oryza Sativa ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Gene Action ◽  
Oryza Sativa L ◽  
Fertility Restoration ◽  
Dominant Gene ◽  
Recessive Gene ◽  
Male Sterile ◽  
Male Sterile Line

Inheritance of fertility restoration was studied in crosses involving ten elite restorer lines of rice viz. BR6839-41-5-1R, BR7013-62-1-1R, BR7011-37-1-2R, BR10R, BR11R, BR12R, BR13R, BR14R, BR15R and BR16R and one male sterile line Jin23A with WA sources of cytoplasmic male sterility. The segregation pattern for pollen fertility of F2 and BC1 populations of crosses involving Jin23A indicated the presence of two independent dominant fertility restoring genes. The mode of action of the two genes varied in different crosses revealing three types of interaction, i.e. epistasis with dominant gene action, epistasis with recessive gene action, and epistasis with incomplete dominance.DOI: http://dx.doi.org/10.3329/bjpbg.v24i1.16997

Genetic analysis and molecular mapping of a novel gene for zebra mutation in rice (Oryza sativa L.)

2009 ◽  
Vol 36 (11) ◽  
pp. 679-684 ◽  
Author(s):  
Qiushi Wang ◽  
Xianchun Sang ◽  
Yinghua Ling ◽  
Fangming Zhao ◽  
Zhenglin Yang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Genetic Analysis ◽  
Molecular Mapping ◽  
Oryza Sativa L ◽  
Novel Gene

scholarly journals Genetic Analysis and Related Gene Primary Mapping of Heat Stress Tolerance in Cucumber Using Bulked Segregant Analysis

HortScience ◽  
2019 ◽  
Vol 54 (3) ◽  
pp. 423-428
Author(s):  
Min Wang ◽  
Wenrui Liu ◽  
Biao Jiang ◽  
Qingwu Peng ◽  
Xiaoming He ◽  
...  
Keyword(s):  
Heat Stress ◽  
Genetic Analysis ◽  
Candidate Genes ◽  
High Temperatures ◽  
Genomic Sequence ◽  
Bulked Segregant Analysis ◽  
Recessive Gene ◽  
Chromosome 1 ◽  
Isolation And Characterization ◽  
Indel Markers

Heat stress (HS) negatively influences plant development and growth, especially production and quality. Cucumber is a widely cultivated plant in the gourd family Cucurbitaceae that is often exposed to high temperatures during summer and protected cultivation. In this study, we performed whole-genome re-sequencing of two pools, one heat-tolerant and one heat-sensitive, of the F2 population derived from L-9 (heat-resistant) and A-16 (heat-sensitive). The genetic analysis showed that the heat resistance of L-9 cucumber seedlings was controlled by a single recessive gene. By combining bulked segregant analysis (BSA) technology, the crucial gene related to HS was preliminarily mapped to a 1.08-Mb region on chromosome 1. To fine-map the locus, Indel markers were designed according to the genomic sequence. Finally, the gene was narrowed to a 550-kb region flanked by two Indel markers, namely Indel-H90 and Indel-H224, that contained 56 candidate genes. Re-sequencing results indicated that 10 candidate genes among the 56 in the candidate region showed single base pair differences in the exons. Quantitative reverse-transcription polymerase chain reaction showed that 6 genes among the 10 candidate genes were significantly decreased when exposed to high temperatures. These results not only were useful for the isolation and characterization of the key genes involved in HS but also provided a basis for understanding the mechanism of heat tolerance regulation.

scholarly journals Molecular Mapping of a Recessive Powdery Mildew Resistance Gene in Wheat Cultivar Tian Xuan 45 Using Bulked Segregant Analysis with Polymorphic Single Nucleotide Polymorphism Relative Ratio Distribution

2019 ◽  
Vol 109 (5) ◽  
pp. 828-838 ◽  
Author(s):  
Kaixiang Chao ◽  
Wenwen Su ◽  
Lei Wu ◽  
Bei Su ◽  
Qiang Li ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Molecular Mapping ◽  
Wheat Cultivar ◽  
Bulked Segregant Analysis ◽  
Recessive Gene ◽  
Wheat Cultivars ◽  
Ratio Distribution ◽  
Single Nucleotide ◽  
Mildew Resistance

Powdery mildew is a destructive foliar disease of wheat worldwide. Wheat cultivar Tian Xuan 45 exhibits resistance to the highly virulent isolate HY5. Genetic analysis of the F2 and F2:3 populations of a cultivar Ming Xian 169/Tian Xuan 45 cross revealed that the resistance to HY5 was controlled by a single recessive gene, temporarily designated as PmTx45. A Manhattan plot with the relative frequency distribution of single nucleotide polymorphisms (SNPs) was used to rapidly narrow down the possible chromosomal regions of the associated genes. This microarray-based bulked segregant analysis (BSA) largely improved traditional analytical methods. PmTx45 was located in chromosomal bin 4BL5-0.86-1.00 and was flanked by SNP marker AX-110673642 and intron length polymorphism (ILP) marker ILP-4B01G269900 with genetic distances of 3.0 and 2.6 cM, respectively. Molecular detection in a panel of wheat cultivars using the markers linked to PmTx45 showed that the presence of PmTx45 in commercial wheat cultivars was rare. Resistance spectrum and chromosomal position analyses indicated that PmTx45 may be a novel recessive gene with moderate powdery mildew resistance. This new microarray-based BSA method is feasible and effective and has the potential application for mapping genes in wheat in marker-assisted breeding.

Alloplasmic male sterile Brassica napus with Enarthrocarpus lyratus cytoplasm: introgression and molecular mapping of an E. lyratus chromosome segment carrying a fertility restoring gene

Genome ◽  
2003 ◽  
Vol 46 (5) ◽  
pp. 792-797 ◽  
Author(s):  
H S Janeja ◽  
S K Banga ◽  
P B Bhaskar ◽  
S S Banga
Keyword(s):  
Brassica Napus ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Molecular Mapping ◽  
Bulked Segregant Analysis ◽  
Fertility Restoration ◽  
Hybrid Seed Production ◽  
Male Sterile ◽  
Fertility Restorer ◽  
Donor Species

A cytoplasmic male sterility (CMS) system for Brassica napus (2n = 38; AACC) was developed by backcross substitution of its nucleus into the cytoplasm of a wild crucifer, Enarthrocarpus lyratus. Male sterility was complete, stable, and expressed in small flowers with rudimentary anthers. Since the B. napus germplasm lines were complete or partial maintainers of male sterility, the required fertility restorer gene (Rfl) was introgressed from the cytoplasm donor species. Inheritance studies carried out on F1 and F2 populations derived from hybridizing cytoplasmic male sterile and male fertile near-isogenic (PNILs) lines of B. napus 'Westar', revealed a monogenic dominant control for fertility restoration. Bulked segregant analysis with 215 RAPD primers helped in the identification of putative primers associated with fertility restoration. Co-segregation analysis of eight such primers with Rfl gene revealed two markers, OPK 15700 and OPZ 061300, which flank the Rfl locus on either side at a distance of 8.2 and 2.5 cM, respectively. These DNA markers will be useful in marker-assisted selection for improving the commercial potential of this newly developed CMS-fertility-restorer system for hybrid seed production programs in rapeseed.Key words: oilseed rape, hybrids, cytoplasmic male sterility, fertility restoration, RAPD mapping.

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