Karyotypic and chromosome banding studies of the potato tuber moth, Phthorimaea operculella (Zeller) (Lepidoptera, Gelechiidae)

The karyotype of Phthorimaea operculella is similar in both sexes and consists of 29 chromosome pairs. These are of similar size with gradual intergradation except for one pair which is significantly longer. C-banding and fluorescent staining with quinacrine and Hoechst 33258 failed to induce bands in metaphase chromosomes while silver staining clearly showed active nucleoli in all stages except metaphase. The banding results are compared with the few reports available on banding of holocentric chromosomes. It is concluded that considerable variability exists in the heterochromatic structure of holocentric chromosomes.Key words: Lepidoptera, moth, karyotype, C-banding.

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Sequential chromosome banding and in situ hybridization analysis

Genome ◽

10.1139/g93-104 ◽

1993 ◽

Vol 36 (4) ◽

pp. 792-795 ◽

Cited By ~ 47

Author(s):

Jiming Jiang ◽

Bikram S. Gill

Keyword(s):

In Situ Hybridization ◽

Dna Sequences ◽

Acid Treatment ◽

Chromosome Banding ◽

Molecular Mapping ◽

Genome Mapping ◽

Genomic In Situ Hybridization ◽

Metaphase Chromosomes ◽

C Banding

Different combinations of chromosome N- or C-banding with in situ hybridization (ISH) or genomic in situ hybridization (GISH) were sequentially performed on metaphase chromosomes of wheat. A modified N-banding–ISH/GISH sequential procedure gave best results. Similarly, a modified C-banding – ISH/GISH procedure also gave satisfactory results. The variation of the hot acid treatment in the standard chromosome N- or C-banding procedures was the major factor affecting the resolution of the subsequent ISH and GISH. By the sequential chromosome banding – ISH/GISH analysis, multicopy DNA sequences and the breakpoints of wheat–alien translocations were directly allocated to specific chromosomes of wheat. The sequential chromosome banding– ISH/GISH technique should be widely applicable in genome mapping, especially in cytogenetic and molecular mapping of heterochromatic and euchromatic regions of plant and animal chromosomes.Key words: N-banding, C-banding, in situ hybridization, genomic in situ hybridization.

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DIFFERENTIAL GIEMSA STAINING IN PLANTS. VI. CENTROMERIC BANDING

Canadian Journal of Genetics and Cytology ◽

10.1139/g79-041 ◽

1979 ◽

Vol 21 (3) ◽

pp. 373-378 ◽

Cited By ~ 9

Author(s):

W. Gary Filion ◽

David H. Blakey

Keyword(s):

Room Temperature ◽

Chromosome Banding ◽

Giemsa Staining ◽

Barium Hydroxide ◽

Banding Technique ◽

Giemsa Banding ◽

Metaphase Chromosomes ◽

Somatic Metaphase ◽

C Banding ◽

Hydrolysis Temperature

Somatic metaphase chromosomes of Tulipa which were subjected to various hydrolyses with several times and temperatures displayed two distinctive types of C-banding when stained using the BSG (Barium hydroxide/Saline/Giemsa) chromosome banding technique. In addition to the two types of Giemsa bands, namely intercalary/terminal and centromeric, a unique transition from the former to the latter type of banding was observed. That is, at the point of transition from intercalary/terminal to centromeric banding, both types were present at one time. The two types of Giemsa banding resulted from different HCl hydrolysis times and temperatures; centromeric bands being observed after either a prolonged hydrolysis at room temperature or an increase in the hydrolysis temperature to 60 °C. These results are discussed in relation to the mechanisms of chromosome banding.

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Botanical extracts against the potato tuber moth, Phthorimaea operculella (Zeller 1873) (Lepidoptera: Gelechiidae), during storage conditions

Egyptian Journal of Biological Pest Control ◽

10.1186/s41938-020-00294-1 ◽

2020 ◽

Vol 30 (1) ◽

Author(s):

Aziza Mohamed Fouad Sharaby ◽

Mohamed Ahmed Gesraha ◽

Sahar Ahmed Baker Fallatah

Keyword(s):

Potato Tuber ◽

Storage Conditions ◽

Phthorimaea Operculella ◽

Potato Tuber Moth ◽

Botanical Extracts

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Expression of various biotin-binding proteins in transgenic tobacco confers resistance to potato tuber moth, Phthorimaea operculella (Zeller) (fam. Gelechiidae)

Transgenic Research ◽

10.1007/s11248-010-9380-y ◽

2010 ◽

Vol 19 (6) ◽

pp. 1041-1051 ◽

Cited By ~ 8

Author(s):

Colleen Murray ◽

Ngaire P. Markwick ◽

Ryohei Kaji ◽

Joanne Poulton ◽

Harry Martin ◽

...

Keyword(s):

Potato Tuber ◽

Transgenic Tobacco ◽

Binding Proteins ◽

Phthorimaea Operculella ◽

Potato Tuber Moth ◽

Biotin Binding

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Rye C-banding patterns and meiotic stability of hexaploid triticale (× Triticosecale) selections differing in kernel shriveling

Genome ◽

10.1139/g90-103 ◽

1990 ◽

Vol 33 (5) ◽

pp. 686-689 ◽

Cited By ~ 1

Author(s):

Charles M. Papa ◽

R. Morris ◽

J. W. Schmidt

Keyword(s):

Secale Cereale ◽

Chromosome Banding ◽

Agronomic Performance ◽

Hexaploid Triticale ◽

Kernel Development ◽

Banding Patterns ◽

C Banding ◽

Meiotic Stability ◽

Metaphase I

Two winter hexaploid triticale populations derived from the same cross were selected on the basis of grain appearance and agronomic performance. The five lines from 84LT402 showed more kernel shriveling than the four lines from 84LT401. The derived lines were analyzed for aneuploid frequencies, rye chromosome banding patterns, and meiotic stability to detect associations with kernel development. The aneuploid frequencies were 16% in 84LT401 and 18% in 84LT402. C-banding showed that both selection groups had all the rye chromosomes except 2R. The two groups had similar telomeric patterns but differed in the long-arm interstitial patterns of 4R and 5R. Compared with lines from 84LT402, those from 84LT401 had significantly fewer univalents and rod bivalents, and more paired arms at metaphase I; fewer laggards and bridges at anaphase I; and a higher frequency of normal tetrads. There were no significant differences among lines within each group for any meiotic character. Since there were no differences within or between groups in telomeric banding patterns, the differences in kernel shriveling and meiotic stability might be due to genotypic factors and (or) differences in the interstitial patterns of 4R and 5R. By selecting plump grains, lines with improved kernel characteristics along with improved meiotic stability are obtainable.Key words: triticale, meiotic stability, C-banding, Secale cereale, heterochromatin.

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