Transposition of minisatellite-like DNA in Chironomus midges

Cla elements are a family of tandem repetitive DNA sequences present in the genome of several Chironomus species. Interspersed clusters of Cla elements are widely distributed all over the chromosomes in C. thummi thummi, while they seem to be limited to the centromeric regions in the closely related subspecies C. t. piger. Here we present molecular evidence that this differential distribution is due to a transposition of Cla elements during evolution of the C. t. thummi genome. We have cloned a "filled" integration site (containing a Cla element cluster) from C. t. thummi and the corresponding "empty" genomic site from C. t. piger and other related species. The comparison shows that tandem repetitive elements may be mobilized together with flanking DNA.Key words: minisatellites, transposition, Chironomus.

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Physical chromosome mapping of repetitive DNA sequences in Nile tilapia Oreochromis niloticus: Evidences for a differential distribution of repetitive elements in the sex chromosomes

Micron ◽

10.1016/j.micron.2007.02.010 ◽

2008 ◽

Vol 39 (4) ◽

pp. 411-418 ◽

Cited By ~ 51

Author(s):

Irani A. Ferreira ◽

Cesar Martins

Keyword(s):

Repetitive Dna ◽

Sex Chromosomes ◽

Dna Sequences ◽

Oreochromis Niloticus ◽

Nile Tilapia ◽

Chromosome Mapping ◽

Repetitive Elements ◽

Repetitive Dna Sequences ◽

Differential Distribution ◽

Nile Tilapia Oreochromis Niloticus

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Long-Term Rasamsonia argillacea Complex Species Colonization Revealed by PCR Amplification of Repetitive DNA Sequences in Cystic Fibrosis Patients

Journal of Clinical Microbiology ◽

10.1128/jcm.01462-16 ◽

2016 ◽

Vol 54 (11) ◽

pp. 2804-2812 ◽

Cited By ~ 10

Author(s):

Abdelmounaim Mouhajir ◽

Olivier Matray ◽

Sandrine Giraud ◽

Laurent Mély ◽

Christophe Marguet ◽

...

Keyword(s):

Cystic Fibrosis ◽

Repetitive Dna ◽

Dna Sequences ◽

Species Complex ◽

Pcr Amplification ◽

Patient Specific ◽

Molecular Evidence ◽

Repetitive Dna Sequences ◽

Complex Species ◽

Content Type

The aim of this work was to document molecular epidemiology of Rasamsonia argillacea species complex isolates from cystic fibrosis (CF) patients. In this work, 116 isolates belonging to this species complex and collected from 26 CF patients and one patient with chronic granulomatous disease were characterized using PCR amplification assays of repetitive DNA sequences and electrophoretic separation of amplicons (rep-PCR). Data revealed a clustering consistent with molecular species identification. A single species was recovered from most patients. Rasamsonia aegroticola was the most common species, followed by R. argillacea sensu stricto and R. piperina , while R. eburnea was not identified. Of 29 genotypes, 7 were shared by distinct patients while 22 were patient specific. In each clinical sample, most isolates exhibited an identical genotype. Genotyping of isolates recovered from sequential samples from the same patient confirmed the capability of R. aegroticola and R. argillacea isolates to chronically colonize the airways. A unique genotype was recovered from two siblings during a 6-month period. In the other cases, a largely dominant genotype was detected. Present results which support the use of rep-PCR for both identification and genotyping for the R. argillacea species complex provide the first molecular evidence of chronic airway colonization by these fungi in CF patients.

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Diversity and evolution of four dispersed repetitive DNA sequences in the genus Secale

Genome ◽

10.1139/g10-118 ◽

2011 ◽

Vol 54 (4) ◽

pp. 285-300 ◽

Cited By ~ 6

Author(s):

Zong-Xiang Tang ◽

Shu-Lan Fu ◽

Zheng-Long Ren ◽

Tao Zhang ◽

Yu-Ting Zou ◽

...

Keyword(s):

Repetitive Dna ◽

Dna Sequences ◽

Wild Species ◽

Single Case ◽

Repetitive Elements ◽

Repetitive Dna Sequences ◽

Genomic Evolution ◽

Cultivated Species ◽

Wild Accessions

We present the first characterization of 360 sequences in six species of the genus Secale of both cultivated and wild accessions. These include four distinct kinds of dispersed repetitive DNA sequences named pSc20H, pSc119.1, pSaO5411, and pSaD15940 belonging to the Revolver family. During the evolution of the genus Secale from wild to cultivated accessions, the pSaO5411-like sequences became shorter mainly because of the deletion of a trinucleotide tandem repeating unit, the pSc20H-like sequences displayed apparent homogenization in cultivated rye, and the second intron of Revolver became longer. In addition, the pSc20H-, pSc119.1-, and pSaO5411-like sequences cloned from wild rye and cultivated rye could be divided into two large clades. No single case of the four kinds of repetitive elements has been inherited by each Secale accession from a lone ancestor. It is reasonable to consider the vertical transmission of the four repetitive elements during the evolution of the genus Secale. The pSc20H- and pSaO5411-like sequences showed evolutionary elimination at specific chromosomal locations from wild species to cultivated species. These cases imply that different repetitive DNA sequences have played different roles in the chromosome development and genomic evolution of rye. The present study adds important information to the investigations dealing with characterization of dispersed repetitive elements in wild and cultivated rye.

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