Variations in the heat-induced protein pattern of several Drosophila montium subgroup species (Diptera: Drosophilidae)

Genome ◽  
1997 ◽  
Vol 40 (1) ◽  
pp. 132-137 ◽  
Author(s):  
Irene Konstantopoulou ◽  
Elena Drosopoulou ◽  
Zacharias G. Scouras

After temperature elevation, the newly synthesized polypeptides from several Drosophila montium subgroup species, of the melanogaster species group, were analyzed in denaturing acrylamide gels. The pattern obtained is characteristic of the heat shock response already documented for many other Drosophila species, although the relative electrophoretic mobility of the "small" heat shock proteins exhibits a species-specific pattern. Based on the above pattern, the montium species are placed in three distinct groups. The present data is consistent with that previously used to propose a northeast to southwest evolutionary mode of expansion for the montium subgroup species.Key words: heat shock proteins, Drosophila montium subgroup species, evolution.

2011 ◽  
Vol 108 (17) ◽  
pp. 6915-6920 ◽  
Author(s):  
M. Miot ◽  
M. Reidy ◽  
S. M. Doyle ◽  
J. R. Hoskins ◽  
D. M. Johnston ◽  
...  

2003 ◽  
Vol 15 (1) ◽  
pp. 33-41 ◽  
Author(s):  
Jean Luiz Simões-Araújo ◽  
Norma Gouvêa Rumjanek ◽  
Márcia Margis-Pinheiro

Plants respond to temperature stress by synthesizing a set of heat shock proteins (HSPs), which may be responsible for the acquisition of thermotolerance. In this study, the induction of small HSPs (sHSPs) in eight common bean varieties was evaluated by Northern blot analysis using the W HSP 16.9 cDNA as heterologous probe. Cowpea was used, as a positive control since this plant, as opposed to common bean, is known to grow well under high temperature regimes such as that found in the Brazilian semi-arid region. After the growth period, the plants were submitted to two h of heat shock at 40 ºC. All varieties tested were able to induce sHSP mRNAs that hybridized with W HSP 16.9 probe. However, significant kinetic differences were found when comparing different varieties. SHSP mRNA levels induced after heat shock in cowpea was higher than the levels observed on the bean varieties displaying the highest expression of these proteins. Besides, the sHSP expression was also assessed at the protein accumulation level by Western-blot analysis for cowpea and both IPA 7 and Negro Argel varieties of bean plants. The revealed protein pattern confirmed that sHSPs are differentially expressed in distinct varieties of common bean according their heat stress tolerance.


1990 ◽  
Vol 80 (2) ◽  
pp. 301-306
Author(s):  
Tiina Vahala ◽  
Tage Eriksson ◽  
Peter Engstrom

Sign in / Sign up

Export Citation Format

Share Document