Identification and mapping of a leaf rust resistance gene in barley line Q21861

Barley line Q21861 possesses an incompletely dominant gene (RphQ) for resistance to leaf rust caused by Puccinia hordei. To investigate the allelic and linkage relations between RphQ and other known Rph genes, F2 populations from crosses between Q21861 and donors of Rph1 to Rph14 (except for Rph8) were evaluated for leaf rust reaction at the seedling stage. Results indicate that RphQ is either allelic with or closely linked to the Rph2 locus. A doubled haploid population derived from a cross between Q21861 and SM89010 (a leaf rust susceptible line) was used for molecular mapping of the resistance locus. Bulked segregant analysis was used to identify markers linked to RphQ, using random amplified polymorphic DNAs (RAPDs), restriction fragment length polymorphisms (RFLPs), and sequence tagged sites (STSs). Of 600 decamer primers screened, amplified fragments generated by 9 primers were found to be linked to the RphQ locus; however, only 4 of them were within 10 cM of the target. The RphQ locus was mapped to the centromeric region of chromosome 7, with a linkage distance of 3.5 cM from the RFLP marker CDO749. Rrn2, an RFLP clone from the ribosomal RNA intergenic spacer region, was found to be very closely linked with RphQ, based on bulked segregant analysis. An STS marker, ITS1, derived from Rrn2, was also closely linked (1.6 cM) to RphQ.Key words: Hordeum vulgare, Puccinia hordei, allelism testing, linkage, molecular markers.

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Evaluation and Mapping of a Leaf Rust Resistance Gene Derived from Hordeum vulgare subsp. spontaneum

Czech Journal of Genetics and Plant Breeding ◽

10.17221/3704-cjgpb ◽

2011 ◽

Vol 40 (No. 3) ◽

pp. 86-90 ◽

Cited By ~ 5

Author(s):

D. Kopahnke ◽

M. Nachtigal ◽

Ordon ◽

F ◽

J. Steffenson B

Keyword(s):

Leaf Rust ◽

Resistance Gene ◽

Rust Resistance ◽

Bulked Segregant Analysis ◽

Dominant Gene ◽

Leaf Rust Resistance ◽

Rust Resistance Gene ◽

Puccinia Hordei ◽

Doubled Haploid Population ◽

Leaf Rust Resistance Gene

Studies of marker development were performed on a doubled haploid population derived from the cross of a highly resistant line H. spontaneum 677 × Krona (susceptible). Previous segregation studies on F2 and F3 populations revealed that the resistance of H. spontaneum 677 was likely due to a single dominant gene. Bulked segregant analysis using AFLPs and SSRs was conducted to identify markers linked to this leaf rust resistance gene. By this approach the resistance gene was located on barley chromosome 2H with the closest markers linked at 6.1 cM (E35M54b) and 13.6 cM (Bmac0218) based on the analysis of 83 DH-lines. In order to get first hints whether this gene may be allelic to rph16 located on chromosome 2H STS marker MWG 2133 co-segregating with rph16 was tested but it turned out to be monomorphic. However, in a resistance test with a set of four different isolates of Puccinia hordei, H. spontaneum 677 showed a different reaction pattern from that of H. spontaneum 680, the source of rph16. Tests of allelism to confirm these results are in progress.

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Genetic analysis and molecular mapping of leaf rust resistance genes in the wheat line 5R618

Czech Journal of Genetics and Plant Breeding ◽

10.17221/164/2014-cjgpb ◽

2014 ◽

Vol 50 (No. 4) ◽

pp. 262-267 ◽

Cited By ~ 3

Author(s):

J. Wang ◽

L. Shi ◽

L. Zhu ◽

X. Li ◽

D. Liu

Keyword(s):

Leaf Rust ◽

Resistance Gene ◽

Rust Resistance ◽

Molecular Mapping ◽

Dominant Gene ◽

Puccinia Triticina ◽

Leaf Rust Resistance ◽

Triticum Aestivum L ◽

Rust Resistance Gene ◽

Leaf Rust Resistance Gene

The wheat (Triticum aestivum L.) line 5R618, bred at the China Agricultural University, is resistant in the seedling stage to the majority of the current Chinese pathotypes of wheat leaf rust (Puccinia triticina). To identify and map the leaf rust resistance gene in the 5R618 line, F2 plants and F2:3 families from a cross between 5R618 and Zhengzhou5389 (susceptible) were inoculated in the greenhouse with the Chinese P. triticina pathotype THJP. Results from the F2 and F2:3 populations indicate that a single dominant gene, temporarily designated Lr5R, conferred resistance. Using the molecular marker method, Lr5R was located on the 3DL chromosome. It was closely linked to the markers Xbarc71 and OPJ-09 with genetic distances of 0.9 cM and 1.0 cM, respectively. At present only one designated gene (Lr24) is located on the 3DL chromosome. The genetic distance between Lr5R and Lr24 confirms that Lr5R is a new leaf rust resistance gene.

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Genetic analysis and molecular mapping of a leaf rust resistance gene in the wheat line 19HRWSN-129

Czech Journal of Genetics and Plant Breeding ◽

10.17221/167/2015-cjgpb ◽

2016 ◽

Vol 52 (No. 1) ◽

pp. 1-5

Author(s):

L. Shi ◽

Z. Li ◽

X. Wang ◽

Z. Kang ◽

L. Zhu ◽

...

Keyword(s):

Genetic Analysis ◽

Leaf Rust ◽

Resistance Gene ◽

Rust Resistance ◽

Molecular Mapping ◽

Leaf Rust Resistance ◽

Rust Resistance Gene ◽

Leaf Rust Resistance Gene ◽

Wheat Line

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Molecular mapping of adult-plant race-specific leaf rust resistance gene Lr12 in bread wheat

Molecular Breeding ◽

10.1007/s11032-010-9467-4 ◽

2010 ◽

Vol 28 (2) ◽

pp. 137-142 ◽

Cited By ~ 26

Author(s):

S. Singh ◽

Robert L. Bowden

Keyword(s):

Leaf Rust ◽

Resistance Gene ◽

Bread Wheat ◽

Rust Resistance ◽

Molecular Mapping ◽

Leaf Rust Resistance ◽

Rust Resistance Gene ◽

Adult Plant ◽

Leaf Rust Resistance Gene

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Bulked Segregant Analysis Identifies Molecular Markers Linked to Melampsora medusae Resistance in Populus deltoides

Phytopathology ◽

10.1094/phyto.2000.90.9.1039 ◽

2000 ◽

Vol 90 (9) ◽

pp. 1039-1042 ◽

Cited By ~ 27

Author(s):

G. M. Tabor ◽

T. L. Kubisiak ◽

N. B. Klopfenstein ◽

R. B. Hall ◽

H. S. McNabb McNabb

Keyword(s):

Molecular Markers ◽

Leaf Rust ◽

Populus Deltoides ◽

Bulked Segregant Analysis ◽

Random Amplified Polymorphic Dna ◽

Genetic Distances ◽

Resistance Locus ◽

The North ◽

Melampsora Medusae ◽

Central United States

In the north central United States, leaf rust caused by Melampsora medusae is a major disease problem on Populus deltoides. In this study we identified molecular markers linked to a M. medusae resistance locus (Lrd1) that was segregating 1:1 within an intraspecific P. deltoides family (C9425DD). Previous field results were confirmed in the controlled environment of a growth chamber through an excised whole-leaf inoculation method. Using bulked segregant analysis we identified two random amplified polymorphic DNA (RAPD) markers (OPG10340 and OPZ191800) that are linked to Lrd1. Based on segregation in a total of 116 progeny, the genetic distances between OPG10340 and OPZ191800 and the resistance locus were estimated as 2.6 and 7.4 Haldane centimorgans (cM), respectively. Multipoint linkage analyses strongly suggest the most likely order for these loci is Lrd1, OPG10340, and OPZ191800. These markers may prove to be instrumental in the eventual cloning of Lrd1, as well as for marker-assisted selection of leaf-rust resistant genotypes.

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Molecular Mapping of Leaf Rust Resistance Gene 5 in Barley

Crop Science ◽

10.2135/cropsci2003.3880 ◽

2003 ◽

Vol 43 (1) ◽

pp. 388 ◽

Cited By ~ 14

Author(s):

J. A. Mammadov ◽

J. C. Zwonitzer ◽

R. M. Biyashev ◽

C. A. Griffey ◽

Y. Jin ◽

...

Keyword(s):

Leaf Rust ◽

Resistance Gene ◽

Rust Resistance ◽

Molecular Mapping ◽

Leaf Rust Resistance ◽

Rust Resistance Gene ◽

Leaf Rust Resistance Gene

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Molecular mapping and markers for leaf rust resistance gene Lr24 in CIMMYT wheat line 19HRWSN-122

Euphytica ◽

10.1007/s10681-015-1469-1 ◽

2015 ◽

Vol 206 (1) ◽

pp. 57-66

Author(s):

Ai-yong Qi ◽

Pei-pei Zhang ◽

Xian-chun Xia ◽

Zhong-hu He ◽

Julio Huerta-Espino ◽

...

Keyword(s):

Leaf Rust ◽

Resistance Gene ◽

Rust Resistance ◽

Molecular Mapping ◽

Leaf Rust Resistance ◽

Rust Resistance Gene ◽

Leaf Rust Resistance Gene ◽

Wheat Line

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Development of a genetic linkage map for Sharon goatgrass (Aegilops sharonensis) and mapping of a leaf rust resistance gene

Genome ◽

10.1139/gen-2013-0065 ◽

2013 ◽

Vol 56 (7) ◽

pp. 367-376 ◽

Cited By ~ 8

Author(s):

P.D. Olivera ◽

A. Kilian ◽

P. Wenzl ◽

B.J. Steffenson

Keyword(s):

Linkage Map ◽

Leaf Rust ◽

Resistance Genes ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Chromosomal Location ◽

Dominant Gene ◽

Leaf Rust Resistance Gene ◽

Linkage Groups ◽

Aegilops Sharonensis

Aegilops sharonensis (Sharon goatgrass), a diploid wheat relative, is known to be a rich source of disease resistance genes for wheat improvement. To facilitate the transfer of these genes into wheat, information on their chromosomal location is important. A genetic linkage map of Ae. sharonensis was constructed based on 179 F2 plants derived from a cross between accessions resistant (1644) and susceptible (1193) to wheat leaf rust. The linkage map was based on 389 markers (377 Diversity Arrays Technology (DArT) and 12 simple sequence repeat (SSR) loci) and was comprised of 10 linkage groups, ranging from 2.3 to 124.6 cM. The total genetic length of the map was 818.0 cM, with an average interval distance between markers of 3.63 cM. Based on the chromosomal location of 115 markers previously mapped in wheat, the four linkage groups of A, B, C, and E were assigned to Ae. sharonensis (Ssh) and homoeologous wheat chromosomes 6, 1, 3, and 2. The single dominant gene (designated LrAeSh1644) conferring resistance to leaf rust race THBJ in accession 1644 was positioned on linkage group A (chromosome 6Ssh) and was flanked by DArT markers wpt-9881 (at 1.9 cM distal from the gene) and wpt-6925 (4.5 cM proximal). This study clearly demonstrates the utility of DArT for genotyping uncharacterized species and tagging resistance genes where pertinent genomic information is lacking.

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