EVIDENCE THAT POTATO VIRUS Y IS CARRIED NEAR THE TIP OF THE STYLETS OF THE APHID VECTOR MYZUS PERSICAE (SULZ.)

1955 ◽  
Vol 1 (9) ◽  
pp. 775-782 ◽  
Author(s):  
R. H. E. Bradley ◽  
R. Y. Ganong
Keyword(s):  
Potato Virus ◽  
Myzus Persicae ◽  
Potato Virus Y ◽  
Wave Band ◽  
Tobacco Plants ◽  
Aphid Vector ◽  
Green Peach Aphids ◽  
Infected Tobacco ◽  
Test Plants

Green peach aphids infective with potato virus Y were made noninfective by exposing the tips of their stylets to ultraviolet radiations in the 2537 Å wave band. Similar results were obtained when the area around the aphid was ventilated during irradiation to carry away the ozone produced by the radiations passing through the air. This irradiation did not appear to harm the aphid even though much of its body was irradiated at the same time. Nor did it affect the infectivity of aphids when their stylets were enclosed by the labium, or when most of the length of the stylets except the tips was exposed to the radiations. Irradiation did, however, reduce the ability of aphids immediately thereafter to acquire potato virus Y during a brief feeding puncture, but this was so whether the tips of the stylets were irradiated or not. Furthermore, an hour after being irradiated, aphids acquired the virus during a brief feeding puncture as readily as did unirradiated controls. It is concluded that irradiation of the tips of the stylets of M. persicae inactivated potato virus Y that otherwise would have been transmitted to the test plants. Only virus near the tips of the stylets appeared to be transmitted even after more than the tips had been inserted into infected tobacco plants.

SOME EFFECTS OF FORMALDEHYDE ON POTATO VIRUS Y IN VITRO, AND ABILITY OF APHIDS TO TRANSMIT THE VIRUS WHEN THEIR STYLETS ARE TREATED WITH FORMALDEHYDE

1955 ◽  
Vol 1 (9) ◽  
pp. 783-793 ◽  
Author(s):  
R. H. E. Bradley ◽  
R. Y. Ganong
Keyword(s):  
Potato Virus ◽  
Myzus Persicae ◽  
Potato Virus Y ◽  
Tobacco Plants ◽  
Considerable Distance ◽  
Formaldehyde Treatment ◽  
Aphid Vectors ◽  
Infected Tobacco

Potato virus Y (PVY) was made noninfective by incubation with formaldehyde in vitro. Yet this noninfective virus reacted with PVY antiserum and caused antibodies to be produced in rabbits as readily as infective PVY. A method is described for baring the stylets of living aphids beyond the end of the labium, which normally encloses the stylets. Specimens of Myzus persicae (Sulz.) infective with PVY were made noninfective by treating the stylets for 30 sec. with concentrations of formaldehyde as low as 0.03%; and 0.25% formaldehyde caused the same effect in five seconds. Aphids were also made noninfective when the proboscis with the tip of the stylets bared was treated with formaldehyde, even after the stylets had been inserted a considerable distance into infected tobacco plants. By contrast, aphids usually remained infective when the proboscis with the stylets enclosed in the labium was treated with similar concentrations of formaldehyde. However, formaldehyde treatment of the stylets did not affect the ability of aphids immediately thereafter to acquire and transmit PVY. These results are compatible with the hypothesis that viruses transmitted like PVY are carried by the stylets of their aphid vectors.

EFFECTS OF DEPTH OF STYLET PENETRATION ON APHID TRANSMISSION OF POTATO VIRUS Y

1956 ◽  
Vol 2 (6) ◽  
pp. 539-547 ◽  
Author(s):  
R. H. E. Bradley
Keyword(s):  
Potato Virus ◽  
Myzus Persicae ◽  
Potato Virus Y ◽  
Plant Cells ◽  
Aphid Transmission ◽  
Tobacco Plants ◽  
Infected Tobacco ◽  
Superficial Tissues

The aphid Myzus persicae (Sulz.) most often became infective with potato virus Y on inserting the stylets only superficially into infected tobacco plants after a period without food; the further the stylets penetrated into the plant, the less likely the aphid was to be infective. When epidermis was removed from infected plants, aphids acquired virus Y as readily from the exposed mesophyll as from the piece of removed epidermis. M. persicae can feed by inserting its stylets through a membrane and into tobacco plants. But aphids rarely became infective with virus Y when they inserted their stylets through a membrane and into infected plants; and infective aphids rarely transmitted the virus when they inserted their stylets through a membrane into susceptible plants. Yet infective aphids that penetrated a membrane could afterwards infect a plant not covered with a membrane. Also, aphids did not become infective when they penetrated the deeper tissues of infected plants that had had the virus in the superficial tissues inactivated by ultraviolet radiations. The main conclusion is that M. persicae rarely becomes infective with virus Y or transmits it after the stylets penetrate beyond the first layer of plant cells.

STUDIES OF THE MECHANISM OF TRANSMISSION OF POTATO VIRUS Y BY THE GREEN PEACH APHID, MYZUS PERSICAE (SULZ.) (HOMOPTERA: APHIDAE)

1954 ◽  
Vol 32 (2) ◽  
pp. 64-73 ◽  
Author(s):  
R. H. E. Bradley
Keyword(s):  
Potato Virus ◽  
Myzus Persicae ◽  
Potato Virus Y ◽  
Green Peach Aphid ◽  
Infected Plant ◽  
Test Plants

Wingless adults of Myzus persicae (Sulz.) usually ceased to be infective within minutes and always within hours after leaving tobacco infected with potato virus Y, the time being longer when the aphids were kept from feeding than when they fed, and aphids that were kept from feeding remained infective longer at 2 °C. than at 35 °C. After one to four hours without food, over 80% of M. persicae made initial feeding punctures that lasted less than a minute, and about 70% of these aphids transmitted potato virus Y after one such feeding puncture on an infected plant. Though virus was acquired by aphids during feeding punctures as brief as five seconds, those that made feeding punctures lasting 11–60 sec. were the most likely to become infective. The percentage of aphids that transmitted potato virus Y decreased when the stylets were inserted into infected plants for over a minute, and none of the aphids transmitted the virus after the stylets had been inserted over 20 min. The highest percentage of aphids transmitted potato virus Y when they were transferred to test plants immediately after a single brief feeding puncture on an infected plant. The percentage of aphids that transmitted the virus decreased when they spent 10 min. or longer on infected plants; also, the probability of their becoming infective during a single feeding puncture decreased by about one third during the first 10 min. they fed after one to four hours without food. Even when conditions were suitable, about 25% of M. persicae failed to transmit potato virus Y. Yet those that failed to transmit the virus in one infection trial transmitted it as readily in a second trial as those that transmitted it in the first.

Potato virus Y-infected tobacco affects the growth, reproduction, and feeding behavior of a vector aphid, Myzus persicae (Hemiptera: Aphididae)

2015 ◽  
Vol 50 (2) ◽  
pp. 239-243 ◽  
Author(s):  
Guang-wei Ren ◽  
Xiu-fang Wang ◽  
Dan Chen ◽  
Xin-wei Wang ◽  
Xiu-juan Fan ◽  
...  
Keyword(s):  
Feeding Behavior ◽  
Potato Virus ◽  
Myzus Persicae ◽  
Potato Virus Y ◽  
Infected Tobacco

scholarly journals Transmission of Potato virus Y in Tobacco Plants by Myzus persicae nicotianae and M. persicae s.str.

Plant Disease ◽  
2006 ◽  
Vol 90 (6) ◽  
pp. 777-782 ◽  
Author(s):  
Olympia M. Kanavaki ◽  
John T. Margaritopoulos ◽  
Nikolaos I. Katis ◽  
Panagiotis Skouras ◽  
John A. Tsitsipis
Keyword(s):  
Potato Virus ◽  
Myzus Persicae ◽  
Host Selection ◽  
Potato Virus Y ◽  
Virus Transmission ◽  
Transmission Efficiency ◽  
Behavioral Differences ◽  
Tobacco Plants ◽  
Probing Behavior ◽  
Inoculation Access Period

The transmission efficiency of Potato virus Y N (PVYN) from and to tobacco plants by Myzus persicae nicotianae, a tobacco specialist, and M. persicae s.str. (Hemiptera: Aphididae), a generalist, was evaluated. In direct transmission tests, 1,004 spring migrants collected as nymphs from peach and 1,434 wingless females from 12 clonal lineages of both taxa were examined. Both the winged and wingless females were starved before being transferred individually for a 3-min acquisition period on infected plants and then moved to healthy plants for a 10-min inoculation access period. Little variation in transmission efficiency was observed. Although not statistically significant, M. persicae nicotianae was slightly more effective. This may suggest similar genetic properties (e.g., virion receptors in mouthparts) between the taxa in relation to virus transmission. M. persicae s.str. transmitted the virus less efficiently when a shorter inoculation period was given. This may indicate differences in probing behavior during the early phase of host selection. Nevertheless, behavioral differences related to host selection have been found using arena tests with infected and healthy tobacco plants where 180 winged females from each taxon were tested. M. persicae s.str. had significantly higher propensity to transmit PVYN than M. persicae nicotianae (31.9 versus 15.3%).

scholarly journals Preference of the aphid Myzus persicae (Hemiptera: Aphididae) for tobacco plants at specific stages of potato virus Y infection

2019 ◽  
Vol 164 (6) ◽  
pp. 1567-1573 ◽  
Author(s):  
Jinyan Liu ◽  
Yingjie Liu ◽  
Philip Donkersley ◽  
Yonghao Dong ◽  
Xi Chen ◽  
...  
Keyword(s):  
Potato Virus ◽  
Myzus Persicae ◽  
Potato Virus Y ◽  
Tobacco Plants

SOME WAYS IN WHICH A PARAFFIN OIL IMPEDES APHID TRANSMISSION OF POTATO VIRUS Y

1963 ◽  
Vol 9 (3) ◽  
pp. 369-380 ◽  
Author(s):  
R. H. E. Bradley
Keyword(s):  
Potato Virus ◽  
Myzus Persicae ◽  
Laboratory Tests ◽  
Potato Virus Y ◽  
Aphid Transmission ◽  
The Third ◽  
Paraffin Oil

In laboratory tests with a light white paraffin oil, transmission of potato virus Y by viruliferous adults of Myzus persicae (Sulz.) was impeded by (a) allowing aphids to probe on a leaf coated with oil, (b) manually touching the end of the labium to an oil-coated leaf, or (c) inserting the bare stylets directly into the oil. The first two of these but not the third also impeded for some minutes the uptake and transmission of virus Y by nonviruliferous aphids. Oil sprayed on plants kept in the laboratory continued to cause these effects for some weeks. If infected plants, used as virus sources for aphids, were coated with oil, transmission from them was impeded. It is still not clear why oil impedes transmission. But the results give good reasons to believe that oil can be used in the field to prevent spread of certain aphid-borne viruses.

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