TIME OF MOVEMENT OF POTATO LEAF-ROLL AND TURNIP-LATENT VIRUSES OUT OF LEAVES OF PHYSALIS FLORIDANA RYDB.

Des études sur l’abondance et la diversité des pucerons ont été menées pendant trois campagnes agricoles au Mali. Sur la base de relevés de bacs jaunes installés dans des cultures de pomme de terre à Kati et à Sikasso, 2 525 pucerons ont été capturés et identifiés. Dix-neuf espèces de pucerons ont été recensées, dont deux qui ont été observéesin situsur la culture :Aphis gossypii(Glover) etMyzus persicae(Sulzer). La plupart de ces espèces sont des ravageurs de cultures et elles contribuent également à la transmission virale. Des échantillons foliaires prélevés dans des parcelles de pomme de terre dans les deux régions ont été testés par la technique ELISA pour la détection des deux principaux virus dommageables, soit lePotato VirusY (PVY) et lePotato Leaf Roll Virus(PLRV). Le taux de plantes virosées dans les deux localités pendant les trois années variait de 19,3 % à 21,8 % pour le PVY, alors qu’il était de 8,5 % à 9,3 % pour le PLRV. L’occurrence de ces maladies virales s’est révélée être très homogène d’une année à l’autre, avec des taux relativement importants. Cette étude est une première quantification dans cette région du Mali de l’importance des relations pucerons vecteurs–virus en culture de pomme de terre.

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Overcoming crossing barriers between nontuber-bearing and tuber-bearing Solanum species: towards potato germplasm enhancement with a broad spectrum of solanaceous genetic resources

Genome ◽

10.1139/g95-004 ◽

1995 ◽

Vol 38 (1) ◽

pp. 27-35 ◽

Cited By ~ 31

Author(s):

K. N. Watanabe ◽

M. Orrillo ◽

S. Vega ◽

J. P. T. Valkonen ◽

E. Pehu ◽

...

Keyword(s):

Wild Species ◽

Embryo Rescue ◽

Leaf Roll ◽

Reproductive Traits ◽

Solanum Species ◽

Interspecific Crosses ◽

Potato Leaf ◽

Germplasm Enhancement ◽

Breeding Lines ◽

Potato Germplasm

The first direct sexual hybrids between diploid nontuber-bearing species and diploid potato breeding lines are reported here. Three nontuberous species of Solanum, S. brevidens, S. etuberosum, and S. fernandezianum, were used for sexual crosses, achieved by a combination of rescue pollinations and embryo rescue. Initial hybrid selection was made using an embryo spot marker, followed by the evaluation of morphological and reproductive traits. Putative hybrids were first tested for resistance to potato leaf roll virus derived from the wild species, and then were tested with molecular markers using species-specific DNA probes. Finally, the tuberization of several 2x hybrids was tested for actual potato germplasm enhancement. These hybrids are unique in terms of their potential to enhance recombination between chromosomes of wild species and those of cultivated potatoes in germplasm utilization, and to exploit the genetic nature of tuber formation. The finding that nontuber-bearing Solanum spp. can be directly crossed with tuber-bearing species also has important implications for the regulatory aspects of the use of genetically modified organisms.Key words: nontuber-bearing Solanum, potato germplasm enhancement, interspecific crosses, chromosome manipulation, inter-EBN crosses, diploid.

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A MILD CHLOROSIS VIRUS OF PHYSALIS FLORIDANA FOUND IN A TURNIP LATENT VIRUS COMPLEX

Canadian Journal of Botany ◽

10.1139/b65-056 ◽

1965 ◽

Vol 43 (5) ◽

pp. 509-518 ◽

Cited By ~ 3

Author(s):

J. P. MacKinnon

Keyword(s):

Host Range ◽

Myzus Persicae ◽

Leaf Roll ◽

Aphid Species ◽

Latent Virus ◽

Potato Leaf ◽

Comparative Tests ◽

Total Transmission ◽

Virus Complex ◽

Efficient Vector

Myzus persicae (Sulz.) transmitted a new virus to healthy Physalis floridana Rydb. from other P. floridana infected with a turnip latent virus complex. Symptoms were characterized by a chlorosis and sometimes cupping of only the lower leaves with slight stunting of plants. These symptoms could easily be mistaken for those incited by potato leaf roll virus but they were strikingly different from the yellow netting of plants infected with the turnip latent virus complex.In virus–vector studies with the new virus and M. persicae, an occasional aphid acquired the virus in 2 hours and 14% of infective insects transmitted it in 30 minutes. When acquisition and inoculation feedings were each increased to 48 hours, transmissions by single aphids of 70% and more were common. A few aphids transmitted the virus in a total transmission time of 30 hours, but most required 72 hours or longer to acquire, become infective, and transmit it.In comparative tests between the new virus and either potato leaf roll or the turnip latent complex, the new virus and potato leaf roll were recovered from plants 3 days after inoculation but the complex was not recovered for 14 days or longer. Of five aphid species tested, M. persicae was an efficient vector of all three viruses and the only species to transmit the new virus and the turnip latent complex. Plants infected with the new virus and showing symptoms were as easily infected with potato leaf roll as were comparable controls, but fewer of them became infected with the complex. The new virus and the complex were quite similar in host range, and susceptible plants were found in the Amaranthaceae, Chenopodiaceae, Cruciferae, and Solanaceae.The new virus is called mild chlorosis virus of P. floridana.

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Biodiversity and full genome sequence of potato viruses Alfalfa mosaic virus and potato leaf roll virus in Egypt

Zeitschrift für Naturforschung C ◽

10.1515/znc-2018-0033 ◽

2018 ◽

Vol 73 (11-12) ◽

pp. 423-438 ◽

Cited By ~ 3

Author(s):

Engy E. Abdel Aleem ◽

Radwa M. Taha ◽

Faiza A. Fattouh

Keyword(s):

Phylogenetic Analysis ◽

Nucleotide Sequence ◽

Mosaic Virus ◽

Complete Nucleotide Sequence ◽

Leaf Roll ◽

Alfalfa Mosaic Virus ◽

Potato Leaf Roll Virus ◽

Rt Pcr ◽

Potato Leaf ◽

Potato Viruses

Abstract Solanum tuberosum (potato) is the second most important vegetable crop in Egypt. It is locally consumed, manufactured or supplied for export to Europe and other Arab countries. Potato is subject to infection by a number of plant viruses, which affect its yield and quality. Potato virus Y (PVY), potato leaf roll virus (PLRV), and Alfalfa mosaic virus (AMV) were detected in major potato-growing areas surveyed. Multiplex-RT-PCR assay was used for the detection of these three viruses in one reaction using three specific primer pairs designed to amplify genomic parts of each virus (1594 bp for PLRV, 795 bp for AMV, 801 bp for PVY). All three viruses were detected in a single reaction mixture in naturally infected field-grown potatoes. Multiplex RT-PCR improved sensitivity necessary for the early detection of infection. Incidence of single, double, or triple infection has been recorded in some locations. Full-length sequencing has been performed for an Egyptian FER isolate of PLRV. Through phylogenetic analysis, it was shown to occupy the same clade with isolate JokerMV10 from Germany. Complete nucleotide sequence of an Egyptian FER isolate of AMV and phylogenetic analysis was also performed; we propose that it is a new distinct strain of AMV belonging to a new subgroup IIC. This is the first complete nucleotide sequence of an Egyptian isolate of AMV. Genetic biodiversity of devastating potato viruses necessitates continuous monitoring of new genetic variants of such viruses.

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