Influence of temperature upon levels of virus and an induced antiviral principle in tomato plants systemically infected with tobacco mosaic virus

1969 ◽  
Vol 15 (12) ◽  
pp. 1469-1471 ◽  
Author(s):  
K. C. Chadha ◽  
B. H. MacNeill
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Critical Level ◽  
Diseased Plant ◽  
Nicotiana Glutinosa ◽  
Tomato Plants ◽  
Infected Tomato ◽  
High Level ◽  
Incubation Temperatures

The levels of tobacco mosaic virus (TMV) and an associated antiviral principle (AVP) in infected tomato plants incubated at temperatures ranging from 15.5 C to 32 C were determined. Yield of TMV, as measured spectrophotometrically, reached a maximum at 26.5 C, but fell off markedly at 32 C. Infectivity, as indicated by local-lesion counts on Nicotiana glutinosa, reached its maximum at 23 C then declined substantially at the higher incubation temperatures. Production of AVP in the diseased plants reached a maximum at 26 C and was maintained at a high level at 32 C. Temperatures most favorable for the induction of AVP had an influence both on the quantity and the quality of the virus produced. It is suggested that the presence of a critical level of AVP in the diseased plant not only lowers the yield of TMV, but profoundly reduces the infectivity of the virus which is present.

An antiviral principle from tomatoes systemically infected with tobacco mosaic virus

1969 ◽  
Vol 47 (4) ◽  
pp. 513-518 ◽  
Author(s):  
K. C. Chadha ◽  
B. H. MacNeill
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Diseased Plant ◽  
Tomato Plants ◽  
Early Stages ◽  
Host Interaction ◽  
High Level ◽  
Susceptible Tomato

An antiviral principle (AVP) has been isolated from tomato plants systemically infected with tobacco mosaic virus (TMV). This principle when mixed with TMV inoculum, or applied separately to the host before inoculation, markedly reduces the infectivity of the virus. Induction of the principle in tomato begins during the very early stages of virus–host interaction; later, with the development of a high level of AVP, there is a concomitant decline both in the amount of virus in the diseased plant and in the infectivity of the virus which is present. The production of AVP is not accompanied by overt signs of hypersensitivity or necrosis in the susceptible tomato host.

scholarly journals Short communication: Pepino mosaic virus, a new threat for Serbia’s tomatoes

2020 ◽  
Vol 18 (4) ◽  
pp. e10SC05
Author(s):  
Ivana Stankovic ◽  
Ana Vucurovic ◽  
Katarina Zecevic ◽  
Branka Petrovic ◽  
Danijela Ristic ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Rt Pcr ◽  
Tomato Production ◽  
Pepino Mosaic Virus ◽  
Tomato Plants ◽  
Rapid Spread ◽  
Infected Tomato ◽  
Dark Green ◽  
Relationship Of ◽  
Das Elisa

Aim of study: To report the occurrence of Pepino mosaic virus (PepMV) on tomato in Serbia and to genetically characterize Serbian PepMV isolates.Area of study: Tomato samples showing virus-like symptoms were collected in the Bogojevce locality (Jablanica District, Serbia).Material and methods: Collected tomato samples were assayed by DAS-ELISA using antisera against eight economically important or quarantine tomato viruses. Three selected isolates of naturally infected tomato plants were mechanically transmitted to tomato ‘Novosadski jabučar’ seedlings. For confirmation of PepMV infection, RT-PCR was performed using specific primers PepMV TGB F/PepMV UTR R. Maximum-likelihood phylogenetic tree was constructed with 47 complete CP gene sequences of PepMV to determine the genetic relationship of Serbian PepMV isolates with those from other parts of the world.Main results: The results of DAS-ELISA indicated the presence of PepMV in all tested samples. Mechanically inoculated ‘Novosadski jabučar’ seedlings expressed yellow spots and light and dark green patches, bubbling, and curled leaves. All tested tomato plants were RT-PCR positive for the presence of PepMV. The CP sequence analysis revealed that the Serbian PepMV isolates were completely identical among themselves and shared the highest nucleotide identity of 95.1% (99.2% aa identity) with isolate from Spain (FJ263341). Phylogenetic analysis showed clustering of the Serbian PepMV isolates into CH2 strain, but they formed separate subgroup within CH2 strain.Research highlights: This is the first data of the presence of PepMV in protected tomato production in Serbia. Considering increased incidence and rapid spread in Europe, the presence of PepMV on tomato could therefore represent serious threat to this valuable crop in Serbia.

THE INHIBITING EFFECT OF SOME HETEROCYCLIC AND OTHER ORGANIC COMPOUNDS ON TOBACCO MOSAIC VIRUS

1955 ◽  
Vol 1 (7) ◽  
pp. 549-559 ◽  
Author(s):  
M. Weintraub ◽  
W. G. Kemp
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Organic Compounds ◽  
Virus Multiplication ◽  
Nicotiana Glutinosa ◽  
Inhibiting Effect ◽  
Mechanism Of Inhibition ◽  
Virus Content ◽  
Rate Of Oxygen Consumption ◽  
Main Effects

A number of heterocyclic and miscellaneous organic compounds have been tested for their effectiveness as virus inhibitors by a half-leaf technique using Nicotiana glutinosa and tobacco mosaic virus. Several of these compounds were found to be effective in varying degrees, the main effects being a reduction in the total number of lesions produced, a delay in symptom expression, and a decrease in virus multiplication as indicated by small lesions, on the treated half-leaves. Although nothing is known about the mechanism of inhibition, it can be concluded that to effect inhibition compounds act through the physiology of the host, rather than directly on the virus. The virus content of treated half-leaves has been estimated by measuring their rate of oxygen consumption. These data indicate that the inhibiting compounds affect the metabolism of the host, as reflected in a change in respiration when compared to control half-leaves, and that the symptomless areas on the treated half-leaves do not contain significant concentrations of virus.

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