Stimulation of dextranase production by oxidized dextran

1970 ◽  
Vol 16 (9) ◽  
pp. 841-844 ◽  
Author(s):  
Robert G. Brown
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Enzyme Production ◽  
Inorganic Nitrogen ◽  
Penicillium Funiculosum ◽  
Inorganic Nitrogen Source ◽  
Oxidized Dextran ◽  
Excess Glucose ◽  
Stimulation Of

Penicillium funiculosum, Penicillium lilacinum, and Spicaria violacea produced excellent yields of dextranase if ketodextran replaced dextran as a carbon source. Ketodextrans I and II having degrees of substitution of 2 and 20% respectively were used in this study. P. funiculosum grew equally well on dextran and ketodextran I but less well on ketodextran II. Addition of a readily metabolizable carbohydrate such as glucose, sucrose, or galactose stimulated growth on ketodextran II, resulting in better dextranase production. However, excess glucose reversed this increase in enzyme production. Replacement of an inorganic nitrogen source with an organic one further stimulated dextranase production during growth of P. funiculosum on ketodextran II.

scholarly journals Solid-state fermentation for production of Pectic enzymes by Aspergillus niger

2017 ◽  
Vol 7 (5) ◽  
pp. 17
Author(s):  
Mirza M.V. Baig ◽  
Aniruddha Ratnakar Apastambh
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Nitrogen Source ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Solid Substrate ◽  
Inorganic Nitrogen Source ◽  
Pectic Enzymes

The production of Pectic enzymes by Aspergillus niger was studied under solid state fermentation (SSF). The effect of fermentation condition such as substrate concentration, inoculum volume, incubation time, moistening agent, inducers and organic and inorganic nitrogen sources was studied for enzyme production. Culture conditions were optimized for maximal yield of enzyme. The solid substrate wheat bran was most suitable for pectic enzyme production under SSF. Enzyme production was found maximum after 10 days of incubation. Lactose was found to be most effective as inducer. Gelatin as organic nitrogen source and ammonium nitrate as inorganic nitrogen source yielded high enzyme titres.

scholarly journals Screening of Bacterial Strains for Polygalacturonase Activity: Its Production by Bacillus sphaericus (MTCC 7542)

2010 ◽  
Vol 2010 ◽  
pp. 1-5 ◽  
Author(s):  
Ranveer Singh Jayani ◽  
Surendra Kumar Shukla ◽  
Reena Gupta
Keyword(s):  
Nitrogen Source ◽  
Enzyme Production ◽  
Inorganic Nitrogen ◽  
Casein Hydrolysate ◽  
Bacillus Sphaericus ◽  
Maximum Activity ◽  
Bacterial Strains ◽  
Inorganic Nitrogen Source ◽  
Polygalacturonase Production ◽  
Polygalacturonase Activity

At present almost all the pectinolytic enzymes used for industrial applications are produced by fungi. There are a few reports of pectinase production by bacterial strains. Therefore, in the present study, seventy-four bacterial strains, isolated from soil and rotten vegetable samples, were screened for polygalacturonase production. The strain PG-31, which gave maximum activity, was identified as Bacillus sphaericus (MTCC 7542). Maximal quantities of polygalacturonase were produced when a 16-hours-old inoculum was used at 7.5% (v/v) in production medium and incubated in shaking conditions (160 rpm) for 72 hours. The optimal temperature and pH for bacterial growth and polygalacturonase production were found to be 30∘C and 6.8, respectively. Maximum enzyme production resulted when citrus pectin was used as the carbon source at a concentration of 1.25% (w/v), whereas other carbon sources led to a decrease (30%–70%) in enzyme production. Casein hydrolysate and yeast extract used together as organic nitrogen source gave best results, and ammonium chloride was found to be the most suitable inorganic nitrogen source. The supplementation of media with 0.9% (w/v) D-galacturonic acid led to a 23% increase in activity. Bacillus sphaericus, a bacterium isolated from soil, produced good amount of polygalacturonase activity at neutral pH; hence, it would be potentially useful to increase the yield of banana, grape, or apple juice.

scholarly journals Production of high cellulase yields from polypore fungi in solid-state fermentation using green tea waste

2019 ◽  
Author(s):  
KA Nguyen ◽  
W Penkhrue ◽  
S Lumyong
Keyword(s):  
Solid State ◽  
Nitrogen Source ◽  
Green Tea ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Ph Value ◽  
Inorganic Nitrogen ◽  
Inorganic Nitrogen Source ◽  
Tea Waste ◽  
Optimal Medium

AbstractPolypores are diverse macrofungi that have been extensively studied for their enzyme production capabilities. Presently, these enzymes are being used for many industrial purposes. However, the high-cost associated with their production is the main barrier to their broader application. This work aimed to study the optimal medium and conditions by using solid state fermentation. Seven polypore strains were used for cellulase activity screening. The fermentation experiments were carried out in 250 mL Erlenmeyer flasks with green tea waste as a substrate. Notably, Microporus sp. KA038 showed the best level of activity of 81.8 IU/gds. Various parameters such as temperature on growth, moisture content, nitrogen source, initial pH value, inoculum size and incubation time were considered to determine the optimal conditions for cellulase production. The optimal medium consisted of green tea leaves as a carbon source, beef extract as an organic nitrogen source, and NH4H2PO4 as an inorganic nitrogen source, while pH 7.0 and an incubation temperature of 30°C for 4 days resulted in a high enzyme yield with Microporus sp. KA038.

scholarly journals Production of thermostable extracellular α-amylase by a moderate thermophilic Bacillus licheniformis isolated from Qinarje Hot Spring (Ardebil prov. of Iran)

2017 ◽  
Vol 118 (4) ◽  
Author(s):  
Ali Deljou ◽  
Iman Arezi
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Bacillus Licheniformis ◽  
Bacterial Growth ◽  
Enzyme Production ◽  
Hot Spring ◽  
Basal Medium ◽  
Thermophilic Bacterium ◽  
Inoculum Size ◽  
Amylase Production

Background and Purpose: Amylases are most important industrial enzymes that account for about 30% of the world’s food, feed, fermentation, textile, detergent and cellulosic industries. This study aimed at optimum production of thermostable α-amylase via moderate thermophilic bacterium (Bacillus licheniformis) which was recently isolated from Qinarje Hot spring.Material and Methods: Initially, ability of bacterium for amylase activity was determined by starch hydrolysis test using Gram’s iodine staining. Then bacterial growth pattern and amylase production curves in basal production medium were graphically determined at different time intervals. Finally, effect of different temperature, pH, carbon source, nitrogen source, minerals and inoculum size were studied on bacterial growth and amylase production using turbidimetric and DNS method, respectively.Results: Optimum enzyme production achieved after 84 hours of inoculation from cultures growing at 40 ˚C and pH 9.0 in a medium containing 0.03% (w/v) of CaCl2, compared to the basal medium, results showed that the best enzyme production happened with inoculum size of 4% (v/v). The addition of 1% (w/v) rice husk (as a Carbon source) enhanced enzyme productivity up to 160% and substitution of the peptone and yeast extract with 1% (w/v) of tryptone (as a Nitrogen source) increased the α-amylase production up to 160%.Conclusion: Our findings show that B. licheniformis-AZ2 strain has an ability to produce the thermostable α-amylase which is suitable in starch processing and food industries. To be commercialized, further investigation is required for enhancement of the enzyme production.Keywords: Bacillus licheniformis; Optimization; Basal medium; Agricultural by-products.

Effect of Ammonium Sulphate on the Sporulation of Bacillus thuringiensis subsp. aizawai SN2 (a Local Isolate) during Batch Fermentation

2012 ◽  
Author(s):  
Wan Mohtar Wan Yusoff ◽  
Mohd. Mazmira Mohd. Masri ◽  
Choy Mei Chan
Keyword(s):  
Bacillus Thuringiensis ◽  
Nitrogen Content ◽  
Key Words ◽  
Nitrogen Source ◽  
Ammonium Sulphate ◽  
Batch Fermentation ◽  
Inorganic Nitrogen ◽  
Inorganic Nitrogen Source ◽  
Initial Concentration ◽  
Bacillus Thuringiensis Subsp

Kesan penambahan ammonium sulfat, (NH4)2SO, (sebagai sumber nitrogen bukan organik tunggal) terhadap pensporaan Bacillus thuringiensis subsp. aizawai strain SN2 dalam kultur kelompok telah dikaji. Peratus spora tertinggi (76 %) dikesan selepas 96 j pengkulturan dalam medium yang mengandungi 1.5 gL-1 (NH4)2SO4. Peratus spora tertinggi sebanyak 82 % juga dikesan dalam sampel 96 j apabila medium yang mengandungi 1.5 gL-1 (NH4)2SO4 ditambah dengan 3.0 gL-1 (NH4)2SO4 pada jam keenam selepas mula fermentasi. Peningkatan peratus spora didapati tidak berkaitan dengan jumlah kandungan nitrogen tetapi berkaitan dengan masa penambahan sumber nitrogen. Kata kunci: Bacillus thuringiensis subsp. aizawai strain SN2, masa penambahan nutrien, kultur kelompok The effect of ammonium sulphate (as the sole inorganic nitrogen source) on the sporulation of Bacillus thuringiensis subsp. aizawai strain SN2 was investigated in batch fermentation. The spore percentage of 76 % was achieved at 96 h after inoculation into a medium containing initial cencentration of 1.5 gL-1 ammonium sulphate. In another experiment, the maximum spore percentage of 82 % was obtained after 96 h inoculation in a medium with initial concentration of 1.5 gL-1 (NH4)2SO4 followed by an addition of 3.0 gL-1 (NH4)2SO4 after 6 h of fermentation. The increase in Bacillus thuringiensis spore percentage was not a function of total nitrogen content in the medium but was a function of the time nitrogen being added. Key words: Bacillus thuringiensis subsp. aizawai strain SN2, timing addition, batch fermentation

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