Effect of Ammonium Sulphate on the Sporulation of Bacillus thuringiensis subsp. aizawai SN2 (a Local Isolate) during Batch Fermentation

2012 ◽  
Author(s):  
Wan Mohtar Wan Yusoff ◽  
Mohd. Mazmira Mohd. Masri ◽  
Choy Mei Chan
Keyword(s):  
Bacillus Thuringiensis ◽  
Nitrogen Content ◽  
Key Words ◽  
Nitrogen Source ◽  
Ammonium Sulphate ◽  
Batch Fermentation ◽  
Inorganic Nitrogen ◽  
Inorganic Nitrogen Source ◽  
Initial Concentration ◽  
Bacillus Thuringiensis Subsp

Kesan penambahan ammonium sulfat, (NH4)2SO, (sebagai sumber nitrogen bukan organik tunggal) terhadap pensporaan Bacillus thuringiensis subsp. aizawai strain SN2 dalam kultur kelompok telah dikaji. Peratus spora tertinggi (76 %) dikesan selepas 96 j pengkulturan dalam medium yang mengandungi 1.5 gL-1 (NH4)2SO4. Peratus spora tertinggi sebanyak 82 % juga dikesan dalam sampel 96 j apabila medium yang mengandungi 1.5 gL-1 (NH4)2SO4 ditambah dengan 3.0 gL-1 (NH4)2SO4 pada jam keenam selepas mula fermentasi. Peningkatan peratus spora didapati tidak berkaitan dengan jumlah kandungan nitrogen tetapi berkaitan dengan masa penambahan sumber nitrogen. Kata kunci: Bacillus thuringiensis subsp. aizawai strain SN2, masa penambahan nutrien, kultur kelompok The effect of ammonium sulphate (as the sole inorganic nitrogen source) on the sporulation of Bacillus thuringiensis subsp. aizawai strain SN2 was investigated in batch fermentation. The spore percentage of 76 % was achieved at 96 h after inoculation into a medium containing initial cencentration of 1.5 gL-1 ammonium sulphate. In another experiment, the maximum spore percentage of 82 % was obtained after 96 h inoculation in a medium with initial concentration of 1.5 gL-1 (NH4)2SO4 followed by an addition of 3.0 gL-1 (NH4)2SO4 after 6 h of fermentation. The increase in Bacillus thuringiensis spore percentage was not a function of total nitrogen content in the medium but was a function of the time nitrogen being added. Key words: Bacillus thuringiensis subsp. aizawai strain SN2, timing addition, batch fermentation

Stimulation of dextranase production by oxidized dextran

1970 ◽  
Vol 16 (9) ◽  
pp. 841-844 ◽  
Author(s):  
Robert G. Brown
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Enzyme Production ◽  
Inorganic Nitrogen ◽  
Penicillium Funiculosum ◽  
Inorganic Nitrogen Source ◽  
Oxidized Dextran ◽  
Excess Glucose ◽  
Stimulation Of

Penicillium funiculosum, Penicillium lilacinum, and Spicaria violacea produced excellent yields of dextranase if ketodextran replaced dextran as a carbon source. Ketodextrans I and II having degrees of substitution of 2 and 20% respectively were used in this study. P. funiculosum grew equally well on dextran and ketodextran I but less well on ketodextran II. Addition of a readily metabolizable carbohydrate such as glucose, sucrose, or galactose stimulated growth on ketodextran II, resulting in better dextranase production. However, excess glucose reversed this increase in enzyme production. Replacement of an inorganic nitrogen source with an organic one further stimulated dextranase production during growth of P. funiculosum on ketodextran II.

scholarly journals Production of high cellulase yields from polypore fungi in solid-state fermentation using green tea waste

2019 ◽  
Author(s):  
KA Nguyen ◽  
W Penkhrue ◽  
S Lumyong
Keyword(s):  
Solid State ◽  
Nitrogen Source ◽  
Green Tea ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Ph Value ◽  
Inorganic Nitrogen ◽  
Inorganic Nitrogen Source ◽  
Tea Waste ◽  
Optimal Medium

AbstractPolypores are diverse macrofungi that have been extensively studied for their enzyme production capabilities. Presently, these enzymes are being used for many industrial purposes. However, the high-cost associated with their production is the main barrier to their broader application. This work aimed to study the optimal medium and conditions by using solid state fermentation. Seven polypore strains were used for cellulase activity screening. The fermentation experiments were carried out in 250 mL Erlenmeyer flasks with green tea waste as a substrate. Notably, Microporus sp. KA038 showed the best level of activity of 81.8 IU/gds. Various parameters such as temperature on growth, moisture content, nitrogen source, initial pH value, inoculum size and incubation time were considered to determine the optimal conditions for cellulase production. The optimal medium consisted of green tea leaves as a carbon source, beef extract as an organic nitrogen source, and NH4H2PO4 as an inorganic nitrogen source, while pH 7.0 and an incubation temperature of 30°C for 4 days resulted in a high enzyme yield with Microporus sp. KA038.

scholarly journals Enhancement of Bacillus subtilis Lipopeptide Biosurfactants Production through Optimization of Medium Composition and Adequate Control of Aeration

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Dhouha Ghribi ◽  
Semia Ellouze-Chaabouni
Keyword(s):  
Bacillus Subtilis ◽  
Nitrogen Source ◽  
Inorganic Nitrogen ◽  
Medium Composition ◽  
High Yield ◽  
Inorganic Nitrogen Source ◽  
Cultural Conditions ◽  
Commercial Applications ◽  
Surfactin Production ◽  
Lipopeptide Biosurfactants

Interest in biosurfactants has increased considerably in recent years, as they are potentially used in many commercial applications in petroleum, pharmaceuticals, biomedical, and food processing industries. Since improvement of their production was of great importance to reduce the final coast, cultural conditions were analyzed to optimize biosurfactants production from Bacillus subtilis SPB1 strain. A high yield of biosurfactants was obtained from a culture of B. subtilis using carbohydrate substrate as a carbon source; among carbohydrates, glucose enhanced the best surfactin production. The optimum glucose concentration was 40 g/L. Higher amount of biosurfactants was obtained using 5 g/L of urea as organic nitrogen source and applying C/N ratio of 7 with ammonium chloride as inorganic nitrogen source. The highest amount of biosurfactants was recorded with the addition of 2% kerosene. Moreover, it was shown, using an automated full-controlled 2.6 L fermenter, that aeration of the medium, which affected strongly the growth regulated biosurfactants synthesis by the producing cell. So that, low or high aerations lead to a decrease of biosurfactants synthesis yields. It was found that when using dissolved oxygen saturation of the medium at 30%, biosurfactants production reached 4.92 g/L.

Sign in / Sign up

Export Citation Format

Share Document