The state of binding of intracellular K+ in Halobacterium cutirubrum

1972 ◽  
Vol 18 (7) ◽  
pp. 993-995 ◽  
Author(s):  
Janos K. Lanyi ◽  
Melvin P. Silverman
Keyword(s):  
Atomic Absorption ◽  
Concentration Gradient ◽  
Equilibrium Dialysis ◽  
The State ◽  
Extreme Halophile ◽  
Dialysis Experiment

Intracellular K+ in lysed freeze-thawed cell pastes of the extreme halophile, H. cutirubrum, diffused readily against a concentration gradient during equilibrium dialysis, whereas the intracellular Mg2+ was not readily diffusible. The K+ activity in the lysed cell pastes, determined directly by K+ ion-specific electrode, was comparable to the total K-content of the cells, as determined by atomic absorption. This finding and the results of the dialysis experiment suggest that the intracellular K+ in H. cutirubrum is not bound.

scholarly journals Combining PET Biodistribution and Equilibrium Dialysis Assays to Assess the Free Brain Concentration and BBB Transport of CNS Drugs

2012 ◽  
Vol 32 (5) ◽  
pp. 874-883 ◽  
Author(s):  
Roger N Gunn ◽  
Scott G Summerfield ◽  
Cristian A Salinas ◽  
Kevin D Read ◽  
Qi Guo ◽  
...  
Keyword(s):  
Drug Development ◽  
Human Brain ◽  
Concentration Gradient ◽  
Equilibrium Dialysis ◽  
Active Efflux ◽  
Brain Concentration ◽  
Drug Candidates ◽  
Positron Emission

The passage of drugs in and out of the brain is controlled by the blood—brain barrier (BBB), typically, using either passive diffusion across a concentration gradient or active transport via a protein carrier. In-vitro and preclinical measurements of BBB penetration do not always accurately predict the in-vivo situation in humans. Thus, the ability to assay the concentration of novel drug candidates in the human brain in vivo provides valuable information for derisking of candidate molecules early in drug development. Here, positron emission tomography (PET) measurements are combined with in-vitro equilibrium dialysis assays to enable assessment of transport and estimation of the free brain concentration in vivo. The PET and equilibrium dialysis data were obtained for 36 compounds in the pig. Predicted P-glycoprotein (P-gp) status of the compounds was consistent with the PET/equilibrium dialysis results. In particular, Loperamide, a well-known P-gp substrate, exhibited a significant concentration gradient consistent with active efflux and after inhibition of the P-gp process the gradient was removed. The ability to measure the free brain concentration and assess transport of novel compounds in the human brain with combined PET and equilibrium dialysis assays can be a useful tool in central nervous system (CNS) drug development.

Practical Picture Processing

1974 ◽  
Vol 32 ◽  
pp. 338-339
Author(s):  
T. A. Welton
Keyword(s):  
Radiation Damage ◽  
Coherence Length ◽  
Spatial Information ◽  
State Of The Art ◽  
Coherent Radiation ◽  
The State ◽  
Energy Spread ◽  
Electron Micrograph ◽  
Picture Processing ◽  
Molecular Skeleton

Various authors have emphasized the spatial information resident in an electron micrograph taken with adequately coherent radiation. In view of the completion of at least one such instrument, this opportunity is taken to summarize the state of the art of processing such micrographs. We use the usual symbols for the aberration coefficients, and supplement these with £ and 6 for the transverse coherence length and the fractional energy spread respectively. He also assume a weak, biologically interesting sample, with principal interest lying in the molecular skeleton remaining after obvious hydrogen loss and other radiation damage has occurred.

Is there a “universal” MAC equation?

1990 ◽  
Vol 48 (2) ◽  
pp. 228-229
Author(s):  
Robert E. Ogilvie
Keyword(s):  
Absorption Coefficient ◽  
Atomic Absorption ◽  
Atomic Number ◽  
X Ray ◽  
Time Period ◽  
Image Position

The search for an empirical absorption equation begins with the work of Siegbahn (1) in 1914. At that time Siegbahn showed that the value of (μ/ρ) for a given element could be expressed as a function of the wavelength (λ) of the x-ray photon by the following equationwhere C is a constant for a given material, which will have sudden jumps in value at critial absorption limits. Siegbahn found that n varied from 2.66 to 2.71 for various solids, and from 2.66 to 2.94 for various gases.Bragg and Pierce (2) , at this same time period, showed that their results on materials ranging from Al(13) to Au(79) could be represented by the followingwhere μa is the atomic absorption coefficient, Z the atomic number. Today equation (2) is known as the “Bragg-Pierce” Law. The exponent of 5/2(n) was questioned by many investigators, and that n should be closer to 3. The work of Wingardh (3) showed that the exponent of Z should be much lower, p = 2.95, however, this is much lower than that found by most investigators.

Pragmatic versus Morphological/Syntactic Criteria for Language Referrals

1980 ◽  
Vol 11 (2) ◽  
pp. 85-94 ◽  
Author(s):  
Jack Damico ◽  
John W. Oller
Keyword(s):  
Grade Level ◽  
The State ◽  
Verb Agreement ◽  
Remedial Programs ◽  
Service Training ◽  
Traditional Approaches ◽  
Communicative Effectiveness

Two methods of identifying language disordered children are examined. Traditional approaches require attention to relatively superficial morphological and surface syntactic criteria, such as, noun-verb agreement, tense marking, pluralization. More recently, however, language testers and others have turned to pragmatic criteria focussing on deeper aspects of meaning and communicative effectiveness, such as, general fluency, topic maintenance, specificity of referring terms. In this study, 54 regular K-5 teachers in two Albuquerque schools serving 1212 children were assigned on a roughly matched basis to one of two groups. Group S received in-service training using traditional surface criteria for referrals, while Group P received similar in-service training with pragmatic criteria. All referrals from both groups were reevaluated by a panel of judges following the state determined procedures for assignment to remedial programs. Teachers who were taught to use pragmatic criteria in identifying language disordered children identified significantly more children and were more often correct in their identification than teachers taught to use syntactic criteria. Both groups identified significantly fewer children as the grade level increased.

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