Localization of melanin in appressoria of Colletotrichum lagenarium

1986 ◽  
Vol 32 (3) ◽  
pp. 280-282 ◽  
Author(s):  
Y. Kubo ◽  
I. Furusawa
Keyword(s):  
Microscopic Observation ◽  
Parent Strain ◽  
Electron Microscopic Observation ◽  
Dense Layer ◽  
Electron Microscopic ◽  
Colletotrichum Lagenarium ◽  
Albino Mutant ◽  
Thin Electron

The location of melanin in appressoria of Colletotrichum lagenarium was determined by ultrastructural comparison of the parent strain 104-T, an albino mutant 79215, and an albino mutant 79215 treated with scytalone, a melanin precursor in this fungus. Melanin in appressoria of the parent strain 104-T and that of albino mutant 79215 treated with scytalone was located external to the plasmalemma as a smooth, thin, electron-dense layer. A comparable layer was not observed in appressoria of the albino mutant 79215 without scytalone. Electron microscopic observation of a median section of pigmented appressoria of albino mutant 79215 treated with scytalone indicated that the melanized layer extended over the appressoria with the exception of the pore which provides an opening for the emergence of the infection peg.

An electron microscopic observation on the germination of conidia of Colletotrichum lagenarium

1968 ◽  
Vol 34 (3-4) ◽  
pp. 337-345 ◽  
Author(s):  
Shigeyasu Akai ◽  
Norio Ishida
Keyword(s):  
Microscopic Observation ◽  
Electron Microscopic Observation ◽  
Electron Microscopic ◽  
Colletotrichum Lagenarium

Electron Microscopic Observation of the Longitudinal Organization of Poly (p-Phenylene Terephthalamide) Fibers

1982 ◽  
Vol 40 ◽  
pp. 680-681
Author(s):  
Li Li-Sheng ◽  
L.F. Allard ◽  
W.C. Bigelow
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Scanning Electron Microscopy ◽  
Microscopic Observation ◽  
Electron Microscopic Observation ◽  
Electron Microscopic ◽  
Aromatic Polyamides ◽  
Radial Arrangement ◽  
Scanning Electron ◽  
Better Than

The aromatic polyamides form a class of fibers having mechanical properties which are much better than those of aliphatic polyamides. Currently, the accepted morphology of these fibers as proposed by M.G. Dobb, et al. is a radial arrangement of pleated sheets, with the plane of the pleats parallel to the axis of the fiber. We have recently obtained evidence which supports a different morphology of this type of fiber, using ultramicrotomy and ion-thinning techniques to prepare specimens for transmission and scanning electron microscopy.

Relation between pigment intensity and penetrating ability in appressoria of Colletotrichum lagenarium

1987 ◽  
Vol 33 (10) ◽  
pp. 870-873 ◽  
Author(s):  
Yasuyuki Kubo ◽  
Iwao Furusawa ◽  
Jiko Shishiyama
Keyword(s):  
Parent Strain ◽  
Colletotrichum Lagenarium ◽  
Melanin Biosynthesis ◽  
Glass Slides ◽  
Albino Mutant

Mutant 8004 of Colletotrichum lagenarium formed slightly pigmented appressoria. The appressoria germinated laterally on glass slides, but could penetrate nitrocellulose membranes. The slightly pigmented appressoria of the albino mutant 79215 were observed to behave in a similar manner when treated with 100 μM scytalone. The appressorial pigmentation of mutant 8004 was increased by the application of scytalone, and mature appressorial pigmentation, indistinguishable from that of the parent strain 104-T, was observed when appressoria were treated with 100 μM scytalone, a lower concentration than that needed for mature pigmentation of the albino mutant. The sensitivity to tricyclazole of appressorial pigmentation of mutant 8004 was higher than that of the parent strain 104-T. These results suggest that, although melanin biosynthesis is essential for appressorial penetration, slight pigmentation is sufficient.

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