Identification of the genes for hydrogenase and cytochrome c3 in Desulfovibrio

1987 ◽  
Vol 33 (11) ◽  
pp. 1006-1010 ◽  
Author(s):  
Gerrit Voordouw ◽  
Helen M. Kent ◽  
John R. Postgate
Keyword(s):  
Cytochrome C ◽  
Sequence Divergence ◽  
Desulfovibrio Vulgaris ◽  
Gene Probe ◽  
Cytochrome C3 ◽  
Sulfate Reducing ◽  
Genes Encoding ◽  
Reducing Bacteria ◽  
Cryptic Plasmids ◽  
Amino Acid Sequence Divergence

Cloned genes encoding cytochrome c3 and hydrogenase from Desulfovibrio vulgaris Hildenborough have been used to probe the genomes of 15 other desulfovibrios. The D. vulgaris strains Wandle and Brockhurst Hill cannot be distinguished from the Hildenborough strain by Southern hybridization using either probe, indicating similar genomes. Desulfovibrio vulgaris Groningen is completely different and lacks homologous cytochrome c3 and hydrogenase genes. The genomes of D. vulgaris ssp. oxamicus Monticello and D. desulfuricans strains El Agheila Z, Berre sol, and Canet 41 contain genes encoding a homologous but not identical periplasmic hydrogenase and cytochrome c3. Weak hybridization was observed with the cytochrome c3 gene probe for genomes of seven other sulfate-reducing bacteria, which reflects the known amino acid sequence divergence of cytochrome c3 in Desulfovibrio. The hydrogenase gene probe shows weak hybridization to the DNA from two strains of D. salexigens only, while the gene may be absent from D. vulgaris Groningen, two strains of D. africanus, D. thermophilus, D. gigas, and D. desulfuricans strains Norway and Teddington R. In desulfovibrios carrying cryptic plasmids the cytochrome c3 and hydrogenase genes are apparently chromosomal.

scholarly journals Newly discovered Asgard archaea Hermodarchaeota potentially degrade alkanes and aromatics via alkyl/benzyl-succinate synthase and benzoyl-CoA pathway

2021 ◽  
Author(s):  
Jia-Wei Zhang ◽  
Hong-Po Dong ◽  
Li-Jun Hou ◽  
Yang Liu ◽  
Ya-Fei Ou ◽  
...  
Keyword(s):  
Nitrate Reduction ◽  
Coenzyme A ◽  
Organic Substrates ◽  
Mangrove Sediments ◽  
Sulfate Reducing ◽  
Sequence Read Archive ◽  
Wide Range ◽  
Genes Encoding ◽  
Sediment Carbon ◽  
Reducing Bacteria

AbstractAsgard archaea are widely distributed in anaerobic environments. Previous studies revealed the potential capability of Asgard archaea to utilize various organic substrates including proteins, carbohydrates, fatty acids, amino acids and hydrocarbons, suggesting that Asgard archaea play an important role in sediment carbon cycling. Here, we describe a previously unrecognized archaeal phylum, Hermodarchaeota, affiliated with the Asgard superphylum. The genomes of these archaea were recovered from metagenomes generated from mangrove sediments, and were found to encode alkyl/benzyl-succinate synthases and their activating enzymes that are similar to those identified in alkane-degrading sulfate-reducing bacteria. Hermodarchaeota also encode enzymes potentially involved in alkyl-coenzyme A and benzoyl-coenzyme A oxidation, the Wood–Ljungdahl pathway and nitrate reduction. These results indicate that members of this phylum have the potential to strictly anaerobically degrade alkanes and aromatic compounds, coupling the reduction of nitrate. By screening Sequence Read Archive, additional genes encoding 16S rRNA and alkyl/benzyl-succinate synthases analogous to those in Hermodarchaeota were identified in metagenomic datasets from a wide range of marine and freshwater sediments. These findings suggest that Asgard archaea capable of degrading alkanes and aromatics via formation of alkyl/benzyl-substituted succinates are ubiquitous in sediments.

Electron transport in sulfate-reducing bacteria. Molecular modeling and NMR studies of the rubredoxin - tetraheme-cytochrome-c3 complex

1989 ◽  
Vol 185 (3) ◽  
pp. 695-700 ◽  
Author(s):  
David E. STEWART ◽  
Jean LEGALL ◽  
Isabel MOURA ◽  
Jose J. G. MOURA ◽  
Harry D. PECK ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Electron Transport ◽  
Sulfate Reducing Bacteria ◽  
Cytochrome C3 ◽  
Nmr Studies ◽  
Sulfate Reducing ◽  
Tetraheme Cytochrome ◽  
Reducing Bacteria

scholarly journals Microbial Corrosion of API 5L X-70 Carbon Steel by ATCC 7757 and Consortium of Sulfate-Reducing Bacteria

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Arman Abdullah ◽  
Nordin Yahaya ◽  
Norhazilan Md Noor ◽  
Rosilawati Mohd Rasol
Keyword(s):  
Weight Loss ◽  
Carbon Steel ◽  
Corrosion Rate ◽  
Sulfate Reducing Bacteria ◽  
Desulfovibrio Vulgaris ◽  
Corrosion Morphology ◽  
Sulfate Reducing ◽  
Weight Loss Method ◽  
Loss Method ◽  
Reducing Bacteria

Various cases of accidents involving microbiology influenced corrosion (MIC) were reported by the oil and gas industry. Sulfate reducing bacteria (SRB) have always been linked to MIC mechanisms as one of the major causes of localized corrosion problems. In this study, SRB colonies were isolated from the soil in suspected areas near the natural gas transmission pipeline in Malaysia. The effects of ATCC 7757 and consortium of isolated SRB upon corrosion on API 5L X-70 carbon steel coupon were investigated using a weight loss method, an open circuit potential method (OCP), and a potentiodynamic polarization curves method in anaerobic conditions. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) were then used to determine the corrosion morphology in verifying the SRB activity and corrosion products formation. Results from the study show that the corrosion rate (CR) of weight loss method for the isolated SRB is recorded as 0.2017 mm/yr compared to 0.2530 mm/yr for ATCC 7757. The Tafel plot recorded the corrosion rate of 0.3290 mm/yr for Sg. Ular SRB and 0.2500 mm/yr forDesulfovibrio vulgaris. The results showed that the consortia of isolated SRB were of comparable effects and features with the single ATCC 7757 strain.

Characterization of the diversity of sulfate-reducing bacteria in soil and mining waste water environments by nucleic acid hybridization techniques

1994 ◽  
Vol 40 (11) ◽  
pp. 955-964 ◽  
Author(s):  
Anita J. Telang ◽  
Gerrit Voordouw ◽  
Sara Ebert ◽  
Neili Sifeldeen ◽  
Julia M. Foght ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Bacterial Communities ◽  
Genomic Dna ◽  
Sulfate Reducing Bacteria ◽  
Oil Field ◽  
Nucleic Acid Hybridization ◽  
Gene Probe ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Field Production

Nucleic acid hybridization techniques were used to characterize the sulfate-reducing bacterial communities at seven waste water and two soil sites in Canada. Genomic DNA was obtained from liquid enrichment cultures of samples taken from these nine sites. The liquid enrichment protocol favored growth of the sulfate-reducing bacterial component of the communities at these sites. The genomic DNA preparations were analyzed with (i) a specific gene probe aimed at a single genus (Desulfovibrio), (ii) a general 16S rRNA gene probe aimed at all genera of sulfate-reducing bacteria and other bacteria, and (iii) whole genome probes aimed at specific bacteria. This three-pronged approach provided information on the sulfate-reducing bacterial community structures for the nine sites. These were compared with each other and with the sulfate-reducing bacterial communities of western Canadian oil field production waters, studied previously. It was found that there is considerable diversity in the sulfate-reducing bacterial community at each site. Most sulfate-reducing bacteria isolated from distinct sites are genomically different and differ also from sulfate-reducing bacteria found in oil field production waters.Key words: sulfate-reducing bacteria, genomic diversity, nucleic acid hybridization, microbial community.

scholarly journals Complete arsenic-based respiratory cycle in the marine microbial communities of pelagic oxygen-deficient zones

2019 ◽  
Vol 116 (20) ◽  
pp. 9925-9930 ◽  
Author(s):  
Jaclyn K. Saunders ◽  
Clara A. Fuchsman ◽  
Cedar McKay ◽  
Gabrielle Rocap
Keyword(s):  
Arsenate Reductase ◽  
Organic Matter Production ◽  
Sulfate Reducing ◽  
Matter Production ◽  
Reductase Gene ◽  
Water Columns ◽  
Large Particles ◽  
Genes Encoding ◽  
Marine Microbial Communities ◽  
Reducing Bacteria

Microbial capacity to metabolize arsenic is ancient, arising in response to its pervasive presence in the environment, which was largely in the form of As(III) in the early anoxic ocean. Many biological arsenic transformations are aimed at mitigating toxicity; however, some microorganisms can respire compounds of this redox-sensitive element to reap energetic gains. In several modern anoxic marine systems concentrations of As(V) are higher relative to As(III) than what would be expected from the thermodynamic equilibrium, but the mechanism for this discrepancy has remained unknown. Here we present evidence of a complete respiratory arsenic cycle, consisting of dissimilatory As(V) reduction and chemoautotrophic As(III) oxidation, in the pelagic ocean. We identified the presence of genes encoding both subunits of the respiratory arsenite oxidase AioA and the dissimilatory arsenate reductase ArrA in the Eastern Tropical North Pacific (ETNP) oxygen-deficient zone (ODZ). The presence of the dissimilatory arsenate reductase gene arrA was enriched on large particles (>30 um), similar to the forward bacterial dsrA gene of sulfate-reducing bacteria, which is involved in the cryptic cycling of sulfur in ODZs. Arsenic respiratory genes were expressed in metatranscriptomic libraries from the ETNP and the Eastern Tropical South Pacific (ETSP) ODZ, indicating arsenotrophy is a metabolic pathway actively utilized in anoxic marine water columns. Together these results suggest arsenic-based metabolisms support organic matter production and impact nitrogen biogeochemical cycling in modern oceans. In early anoxic oceans, especially during periods of high marine arsenic concentrations, they may have played a much larger role.

Microbiologically induced corrosion of stainless steel by Desulfovibrio vulgaris: An scanning electron microscope study

1991 ◽  
Vol 49 ◽  
pp. 28-29
Author(s):  
Roger Garcia ◽  
Fang Li ◽  
Lester Hendrickson
Keyword(s):  
Stainless Steel ◽  
Electron Microscope Study ◽  
Desulfovibrio Vulgaris ◽  
Biological Cell ◽  
Scanning Electron Microscope Study ◽  
Sulfate Reducing ◽  
Corrosion Cell ◽  
Reducing Bacteria ◽  
Microbiologically Induced Corrosion ◽  
Scanning Electron

The corrosion of mild steel by sulfate reducing bacteria has been studied quite extensively. However, with the replacement of mild steels with stainless steel in many of these applications numerous sightings of corroding stainless steel have been made as well. Initially, the cathodic depolarization theory was widely accepted as the mechanism for both. The essential part of this theory involves the removal of hydrogen from the metal surface. Hydrogenase in Desulfovibrio allows utilization of elemental hydrogen from the cathode of the corrosion cell. This causes the reduction of sulfate whereby the biological cell gets its energy via a respiration process. Finally, the oxygen from the sulfate becomes available to the cathode and hence corrosion is enhanced. Without this reducing action the cathode would become polarized thereby decreasing the EMF and lowering the corrosion rate. Among other proposed mechanisms are differential aeration cells and corrosive products produced by the bacteria.

scholarly journals Effect of the Deletion of qmoABC and the Promoter-Distal Gene Encoding a Hypothetical Protein on Sulfate Reduction in Desulfovibrio vulgaris Hildenborough

2010 ◽  
Vol 76 (16) ◽  
pp. 5500-5509 ◽  
Author(s):  
Grant M. Zane ◽  
Huei-che Bill Yen ◽  
Judy D. Wall
Keyword(s):  
Sulfate Reduction ◽  
Electron Acceptor ◽  
Transmembrane Protein ◽  
Hypothetical Protein ◽  
Desulfovibrio Vulgaris ◽  
Gene Encoding ◽  
Terminal Electron Acceptor ◽  
Sulfate Reducing ◽  
Reducing Bacteria

ABSTRACTThe pathway of electrons required for the reduction of sulfate in sulfate-reducing bacteria (SRB) is not yet fully characterized. In order to determine the role of a transmembrane protein complex suggested to be involved in this process, a deletion inDesulfovibrio vulgarisHildenborough was created by marker exchange mutagenesis that eliminated four genes putatively encoding the QmoABC complex and a hypothetical protein (DVU0851). The Qmo (quinone-interactingmembrane-boundoxidoreductase) complex is proposed to be responsible for transporting electrons to the dissimilatory adenosine-5′-phosphosulfate reductase in SRB. In support of the predicted role of this complex, the deletion mutant was unable to grow using sulfate as its sole electron acceptor with a range of electron donors. To explore a possible role for the hypothetical protein in sulfate reduction, a second mutant was constructed that had lost only the gene that codes for the DVU0851 protein. The second constructed mutant grew with sulfate as the sole electron acceptor; however, there was a lag that was not present with the wild-type or complemented strain. Neither deletion strain was significantly impaired for growth with sulfite or thiosulfate as the terminal electron acceptor. Complementation of the Δ(qmoABC-DVU0851) mutant with all four genes or only theqmoABCgenes restored its ability to grow by sulfate respiration. These results confirmed the prediction that the Qmo complex is in the electron pathway for sulfate reduction and revealed that no other transmembrane complex could compensate when Qmo was lacking.

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